Cargando…

Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration

Background and Objectives: Saffron treatment and photobiomodulation (PBM) are non-invasive therapeutic approaches able to mitigate and stabilize retinal degenerative diseases such as age-related macular degeneration (AMD). Although different, these therapies partially match their modulated pattern o...

Descripción completa

Detalles Bibliográficos
Autor principal: Di Paolo, Mattia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538989/
https://www.ncbi.nlm.nih.gov/pubmed/34684096
http://dx.doi.org/10.3390/medicina57101059
_version_ 1784588638317707264
author Di Paolo, Mattia
author_facet Di Paolo, Mattia
author_sort Di Paolo, Mattia
collection PubMed
description Background and Objectives: Saffron treatment and photobiomodulation (PBM) are non-invasive therapeutic approaches able to mitigate and stabilize retinal degenerative diseases such as age-related macular degeneration (AMD). Although different, these therapies partially match their modulated pattern of genes. Recent attempts to find an additive effect by coadministration of saffron and PBM have failed. Instead, in this study, a different protocol to increase neuroprotection by providing consecutive saffron and PBM treatment administration is suggested. Materials and Methods: Albino rats, whose retinal damage was caused by light exposure (LD, light damage), were subjected to differential treatment protocols before and after LD: (1) PBM followed by saffron; and (2) single treatments of PBM. Thinning of the photoreceptor layer and neuro-inflammatory markers for gliosis and microglia were assessed via immune-histochemical techniques. Results: Results confirm that PBM and saffron alone cope with retinal neurodegenerative processes, preserving retinal thickness and gliosis and microglia invasion in a differential way. However, the synergistic effect of the combined treatment was restricted to the early neuroinflammation, even when provided sequentially. Conclusion: The broad spectra of action of both neuroprotectants require further investigation to identify other key pathways helpful in enhancing the effects of these two approaches in combination.
format Online
Article
Text
id pubmed-8538989
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85389892021-10-24 Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration Di Paolo, Mattia Medicina (Kaunas) Article Background and Objectives: Saffron treatment and photobiomodulation (PBM) are non-invasive therapeutic approaches able to mitigate and stabilize retinal degenerative diseases such as age-related macular degeneration (AMD). Although different, these therapies partially match their modulated pattern of genes. Recent attempts to find an additive effect by coadministration of saffron and PBM have failed. Instead, in this study, a different protocol to increase neuroprotection by providing consecutive saffron and PBM treatment administration is suggested. Materials and Methods: Albino rats, whose retinal damage was caused by light exposure (LD, light damage), were subjected to differential treatment protocols before and after LD: (1) PBM followed by saffron; and (2) single treatments of PBM. Thinning of the photoreceptor layer and neuro-inflammatory markers for gliosis and microglia were assessed via immune-histochemical techniques. Results: Results confirm that PBM and saffron alone cope with retinal neurodegenerative processes, preserving retinal thickness and gliosis and microglia invasion in a differential way. However, the synergistic effect of the combined treatment was restricted to the early neuroinflammation, even when provided sequentially. Conclusion: The broad spectra of action of both neuroprotectants require further investigation to identify other key pathways helpful in enhancing the effects of these two approaches in combination. MDPI 2021-10-03 /pmc/articles/PMC8538989/ /pubmed/34684096 http://dx.doi.org/10.3390/medicina57101059 Text en © 2021 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Paolo, Mattia
Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration
title Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration
title_full Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration
title_fullStr Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration
title_full_unstemmed Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration
title_short Sequential PBM–Saffron Treatment in an Animal Model of Retinal Degeneration
title_sort sequential pbm–saffron treatment in an animal model of retinal degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538989/
https://www.ncbi.nlm.nih.gov/pubmed/34684096
http://dx.doi.org/10.3390/medicina57101059
work_keys_str_mv AT dipaolomattia sequentialpbmsaffrontreatmentinananimalmodelofretinaldegeneration