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A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants

Exosomal microRNA (miRNA) in plasma and urine has attracted attention as a novel diagnostic tool for pathological conditions. However, the mechanisms of miRNA dynamics in the exercise physiology field are not well understood in terms of monitoring sports performance. This pilot study aimed to reveal...

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Autores principales: Kuji, Tomoaki, Sugasawa, Takehito, Fujita, Shin-ichiro, Ono, Seiko, Kawakami, Yasushi, Takekoshi, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539081/
https://www.ncbi.nlm.nih.gov/pubmed/34678915
http://dx.doi.org/10.3390/sports9100134
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author Kuji, Tomoaki
Sugasawa, Takehito
Fujita, Shin-ichiro
Ono, Seiko
Kawakami, Yasushi
Takekoshi, Kazuhiro
author_facet Kuji, Tomoaki
Sugasawa, Takehito
Fujita, Shin-ichiro
Ono, Seiko
Kawakami, Yasushi
Takekoshi, Kazuhiro
author_sort Kuji, Tomoaki
collection PubMed
description Exosomal microRNA (miRNA) in plasma and urine has attracted attention as a novel diagnostic tool for pathological conditions. However, the mechanisms of miRNA dynamics in the exercise physiology field are not well understood in terms of monitoring sports performance. This pilot study aimed to reveal the miRNA dynamics in urine and plasma of full-marathon participants. Plasma and urine samples were collected from 26 marathon participants before, immediately after, 2 h after, and one day after a full marathon. The samples were pooled, and exosomal miRNAs were extracted and analyzed using next-generation sequencing. We determined that the exosomal miRNA expression profile changed under time dependency in full marathon. New uncharacterized exosomal miRNAs such as hsa-miR-582-3p and hsa-miR-199a-3p could be potential biomarkers reflecting physical stress of full marathon in plasma and urine. In addition, some muscle miRNAs in plasma and urine have supported the utility for monitoring physical stress. Furthermore, some inflammation-related exosomal miRNAs were useful only in plasma. These results suggest that these exosomal miRNAs in plasma and/or urine are highly sensitive biomarkers for physical stress in full marathons. Thus, our findings may yield valuable insights into exercise physiology.
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spelling pubmed-85390812021-10-24 A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants Kuji, Tomoaki Sugasawa, Takehito Fujita, Shin-ichiro Ono, Seiko Kawakami, Yasushi Takekoshi, Kazuhiro Sports (Basel) Article Exosomal microRNA (miRNA) in plasma and urine has attracted attention as a novel diagnostic tool for pathological conditions. However, the mechanisms of miRNA dynamics in the exercise physiology field are not well understood in terms of monitoring sports performance. This pilot study aimed to reveal the miRNA dynamics in urine and plasma of full-marathon participants. Plasma and urine samples were collected from 26 marathon participants before, immediately after, 2 h after, and one day after a full marathon. The samples were pooled, and exosomal miRNAs were extracted and analyzed using next-generation sequencing. We determined that the exosomal miRNA expression profile changed under time dependency in full marathon. New uncharacterized exosomal miRNAs such as hsa-miR-582-3p and hsa-miR-199a-3p could be potential biomarkers reflecting physical stress of full marathon in plasma and urine. In addition, some muscle miRNAs in plasma and urine have supported the utility for monitoring physical stress. Furthermore, some inflammation-related exosomal miRNAs were useful only in plasma. These results suggest that these exosomal miRNAs in plasma and/or urine are highly sensitive biomarkers for physical stress in full marathons. Thus, our findings may yield valuable insights into exercise physiology. MDPI 2021-09-24 /pmc/articles/PMC8539081/ /pubmed/34678915 http://dx.doi.org/10.3390/sports9100134 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuji, Tomoaki
Sugasawa, Takehito
Fujita, Shin-ichiro
Ono, Seiko
Kawakami, Yasushi
Takekoshi, Kazuhiro
A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
title A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
title_full A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
title_fullStr A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
title_full_unstemmed A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
title_short A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
title_sort pilot study of mirna expression profile as a liquid biopsy for full-marathon participants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539081/
https://www.ncbi.nlm.nih.gov/pubmed/34678915
http://dx.doi.org/10.3390/sports9100134
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