Age-Dependent Honey Bee Appetite Regulation Is Mediated by Trehalose and Octopamine Baseline Levels

SIMPLE SUMMARY: Appetite regulation is an important function necessary to maintain energetic balance, but how honey bees accomplish this could vary as they age because they go through a number of behavioral and physiological changes during development. Here, we determine if the amount of trehalose, which is a sugar found in the hemolymph of honey bees, influences appetite levels and if this interacts with the octopamine neurotransmitter in the bee brain. To accomplish this, we decreased trehalose levels in the hemolymph by injecting an inhibitor of trehalose synthesis. In addition, we increased octopamine levels in the brain by injecting it with a syringe. We found that octopamine and trehalose interact to increase appetite in the two older age classes of bees, beyond just treating the bees with octopamine. The youngest age class did not respond to either treatment. Our results suggest that older honey bees may have an alternative pathway for regulating appetite that uses sugar levels in their hemolymph to communicate to the brain how hungry they are and that octopamine is responsible for elevating appetite levels when the bee is hungry. This pathway is different from how vertebrates regulate their appetite levels based on glucose levels in the blood. ABSTRACT: There are multiple feedback mechanisms involved in appetite regulation, which is an integral part of maintaining energetic homeostasis. Older forager honey bees, in comparison to newly emerged bees and nurse bees, are known to have highly fluctuating hemolymph trehalose levels, higher appetite changes due to starvation, and higher octopamine levels in the brain. What remains unknown is if the hemolymph trehalose and octopamine levels interact with one another and how this varies as the bee ages. We manipulated trehalose and octopamine levels across age using physiological injections and found that nurse and forager bees increase their appetite levels due to increased octopamine levels in the brain. This is further enhanced by lower trehalose levels in the hemolymph. Moreover, nurse bees with high octopamine levels in the brain and low trehalose levels had the same appetite levels as untreated forager bees. Our findings suggest that the naturally higher levels of octopamine as the bee ages may result in higher sensitivity to fluctuating trehalose levels in the hemolymph that results in a more direct way of assessing the energetic state of the individual. Consequently, forager bees have a mechanism for more precise regulation of appetite in comparison to newly emerged and nurse bees.