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Vaginal and Anal Microbiome during Chlamydia trachomatis Infections

Background.Chlamydia trachomatis (CT) is the agent of the most common bacterial sexually transmitted infection worldwide, with a significant impact on women’s health. Despite the increasing number of studies about the vaginal microbiome in women with CT infections, information about the composition...

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Autores principales: Raimondi, Stefano, Candeliere, Francesco, Amaretti, Alberto, Foschi, Claudio, Morselli, Sara, Gaspari, Valeria, Rossi, Maddalena, Marangoni, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539191/
https://www.ncbi.nlm.nih.gov/pubmed/34684295
http://dx.doi.org/10.3390/pathogens10101347
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author Raimondi, Stefano
Candeliere, Francesco
Amaretti, Alberto
Foschi, Claudio
Morselli, Sara
Gaspari, Valeria
Rossi, Maddalena
Marangoni, Antonella
author_facet Raimondi, Stefano
Candeliere, Francesco
Amaretti, Alberto
Foschi, Claudio
Morselli, Sara
Gaspari, Valeria
Rossi, Maddalena
Marangoni, Antonella
author_sort Raimondi, Stefano
collection PubMed
description Background.Chlamydia trachomatis (CT) is the agent of the most common bacterial sexually transmitted infection worldwide, with a significant impact on women’s health. Despite the increasing number of studies about the vaginal microbiome in women with CT infections, information about the composition of the anal microbiome is still lacking. Here, we assessed the bacterial community profiles of vaginal and anal ecosystems associated or not with CT infection in a cohort of Caucasian young women. Methods. A total of 26 women, including 10 with a contemporary vaginal and ano-rectal CT infection, were enrolled. Composition of vaginal and anal microbiome was studied by 16S rRNA gene profiling. Co-occurrence networks of bacterial communities and metagenome metabolic functions were determined. Results. In case of CT infection, both vaginal and anal environments were characterized by a degree of dysbiosis. Indeed, the vaginal microbiome of CT-positive women were depleted in lactobacilli, with a significant increase in dysbiosis-associated bacteria (e.g., Sneathia, Parvimonas, Megasphaera), whereas the anal microbiota of CT-infected women was characterized by higher levels of Parvimonas and Pseudomonas and lower levels of Escherichia. Interestingly, the microbiome of anus and vagina had numerous bacterial taxa in common, reflecting a significant microbial ‘sharing’ between the two sites. In the vaginal environment, CT positively correlated with Ezakiella spp. while Gardnerella vaginalis co-occurred with several dysbiosis-related microbes, regardless of CT vaginal infection. The vaginal microbiome of CT-positive females exhibited a higher involvement of chorismate and aromatic amino acid biosynthesis, as well as an increase in mixed acid fermentation. Conclusions. These data could be useful to set up new diagnostic/prognostic tools, offering new perspectives for the control of chlamydial infections.
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spelling pubmed-85391912021-10-24 Vaginal and Anal Microbiome during Chlamydia trachomatis Infections Raimondi, Stefano Candeliere, Francesco Amaretti, Alberto Foschi, Claudio Morselli, Sara Gaspari, Valeria Rossi, Maddalena Marangoni, Antonella Pathogens Article Background.Chlamydia trachomatis (CT) is the agent of the most common bacterial sexually transmitted infection worldwide, with a significant impact on women’s health. Despite the increasing number of studies about the vaginal microbiome in women with CT infections, information about the composition of the anal microbiome is still lacking. Here, we assessed the bacterial community profiles of vaginal and anal ecosystems associated or not with CT infection in a cohort of Caucasian young women. Methods. A total of 26 women, including 10 with a contemporary vaginal and ano-rectal CT infection, were enrolled. Composition of vaginal and anal microbiome was studied by 16S rRNA gene profiling. Co-occurrence networks of bacterial communities and metagenome metabolic functions were determined. Results. In case of CT infection, both vaginal and anal environments were characterized by a degree of dysbiosis. Indeed, the vaginal microbiome of CT-positive women were depleted in lactobacilli, with a significant increase in dysbiosis-associated bacteria (e.g., Sneathia, Parvimonas, Megasphaera), whereas the anal microbiota of CT-infected women was characterized by higher levels of Parvimonas and Pseudomonas and lower levels of Escherichia. Interestingly, the microbiome of anus and vagina had numerous bacterial taxa in common, reflecting a significant microbial ‘sharing’ between the two sites. In the vaginal environment, CT positively correlated with Ezakiella spp. while Gardnerella vaginalis co-occurred with several dysbiosis-related microbes, regardless of CT vaginal infection. The vaginal microbiome of CT-positive females exhibited a higher involvement of chorismate and aromatic amino acid biosynthesis, as well as an increase in mixed acid fermentation. Conclusions. These data could be useful to set up new diagnostic/prognostic tools, offering new perspectives for the control of chlamydial infections. MDPI 2021-10-19 /pmc/articles/PMC8539191/ /pubmed/34684295 http://dx.doi.org/10.3390/pathogens10101347 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raimondi, Stefano
Candeliere, Francesco
Amaretti, Alberto
Foschi, Claudio
Morselli, Sara
Gaspari, Valeria
Rossi, Maddalena
Marangoni, Antonella
Vaginal and Anal Microbiome during Chlamydia trachomatis Infections
title Vaginal and Anal Microbiome during Chlamydia trachomatis Infections
title_full Vaginal and Anal Microbiome during Chlamydia trachomatis Infections
title_fullStr Vaginal and Anal Microbiome during Chlamydia trachomatis Infections
title_full_unstemmed Vaginal and Anal Microbiome during Chlamydia trachomatis Infections
title_short Vaginal and Anal Microbiome during Chlamydia trachomatis Infections
title_sort vaginal and anal microbiome during chlamydia trachomatis infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539191/
https://www.ncbi.nlm.nih.gov/pubmed/34684295
http://dx.doi.org/10.3390/pathogens10101347
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