Cargando…

Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain

Transient receptor potential vanilloid 1 (TRPV1) has been implicated in peripheral inflammation and is a mediator of the inflammatory response to various noxious stimuli. However, the interaction between TRPV1 and N-methyl-D-aspartate (NMDA) receptors in the regulation of inflammatory pain remains p...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Suk-Yun, Seo, Su Yeon, Bang, Se Kyun, Cho, Seong Jin, Choi, Kwang-Ho, Ryu, Yeonhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539417/
https://www.ncbi.nlm.nih.gov/pubmed/34681836
http://dx.doi.org/10.3390/ijms222011177
_version_ 1784588741976784896
author Kang, Suk-Yun
Seo, Su Yeon
Bang, Se Kyun
Cho, Seong Jin
Choi, Kwang-Ho
Ryu, Yeonhee
author_facet Kang, Suk-Yun
Seo, Su Yeon
Bang, Se Kyun
Cho, Seong Jin
Choi, Kwang-Ho
Ryu, Yeonhee
author_sort Kang, Suk-Yun
collection PubMed
description Transient receptor potential vanilloid 1 (TRPV1) has been implicated in peripheral inflammation and is a mediator of the inflammatory response to various noxious stimuli. However, the interaction between TRPV1 and N-methyl-D-aspartate (NMDA) receptors in the regulation of inflammatory pain remains poorly understood. This study aimed to investigate the analgesic effects of intrathecal administration of capsazepine, a TRPV1 antagonist, on carrageenan-induced inflammatory pain in mice and to identify its interactions with NMDA receptors. Inflammatory pain was induced by intraplantar injection of 2% carrageenan in male ICR mice. To investigate the analgesic effects of capsazepine, pain-related behaviors were evaluated using von Frey filaments and a thermal stimulator placed on the hind paw. TRPV1 expression and NMDA receptor phosphorylation in the spinal cord and glutamate concentration in the spinal cord and serum were measured. Intrathecal treatment with capsazepine significantly attenuated carrageenan-induced mechanical allodynia and thermal hyperalgesia. Moreover, carrageenan-enhanced glutamate and phosphorylation of NMDA receptor subunit 2B in the spinal cord were suppressed by capsazepine administration. These results indicate that TRPV1 and NMDA receptors in the spinal cord are associated with inflammatory pain transmission, and inhibition of TRPV1 may reduce inflammatory pain via NMDA receptors.
format Online
Article
Text
id pubmed-8539417
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85394172021-10-24 Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain Kang, Suk-Yun Seo, Su Yeon Bang, Se Kyun Cho, Seong Jin Choi, Kwang-Ho Ryu, Yeonhee Int J Mol Sci Article Transient receptor potential vanilloid 1 (TRPV1) has been implicated in peripheral inflammation and is a mediator of the inflammatory response to various noxious stimuli. However, the interaction between TRPV1 and N-methyl-D-aspartate (NMDA) receptors in the regulation of inflammatory pain remains poorly understood. This study aimed to investigate the analgesic effects of intrathecal administration of capsazepine, a TRPV1 antagonist, on carrageenan-induced inflammatory pain in mice and to identify its interactions with NMDA receptors. Inflammatory pain was induced by intraplantar injection of 2% carrageenan in male ICR mice. To investigate the analgesic effects of capsazepine, pain-related behaviors were evaluated using von Frey filaments and a thermal stimulator placed on the hind paw. TRPV1 expression and NMDA receptor phosphorylation in the spinal cord and glutamate concentration in the spinal cord and serum were measured. Intrathecal treatment with capsazepine significantly attenuated carrageenan-induced mechanical allodynia and thermal hyperalgesia. Moreover, carrageenan-enhanced glutamate and phosphorylation of NMDA receptor subunit 2B in the spinal cord were suppressed by capsazepine administration. These results indicate that TRPV1 and NMDA receptors in the spinal cord are associated with inflammatory pain transmission, and inhibition of TRPV1 may reduce inflammatory pain via NMDA receptors. MDPI 2021-10-16 /pmc/articles/PMC8539417/ /pubmed/34681836 http://dx.doi.org/10.3390/ijms222011177 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kang, Suk-Yun
Seo, Su Yeon
Bang, Se Kyun
Cho, Seong Jin
Choi, Kwang-Ho
Ryu, Yeonhee
Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain
title Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain
title_full Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain
title_fullStr Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain
title_full_unstemmed Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain
title_short Inhibition of Spinal TRPV1 Reduces NMDA Receptor 2B Phosphorylation and Produces Anti-Nociceptive Effects in Mice with Inflammatory Pain
title_sort inhibition of spinal trpv1 reduces nmda receptor 2b phosphorylation and produces anti-nociceptive effects in mice with inflammatory pain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539417/
https://www.ncbi.nlm.nih.gov/pubmed/34681836
http://dx.doi.org/10.3390/ijms222011177
work_keys_str_mv AT kangsukyun inhibitionofspinaltrpv1reducesnmdareceptor2bphosphorylationandproducesantinociceptiveeffectsinmicewithinflammatorypain
AT seosuyeon inhibitionofspinaltrpv1reducesnmdareceptor2bphosphorylationandproducesantinociceptiveeffectsinmicewithinflammatorypain
AT bangsekyun inhibitionofspinaltrpv1reducesnmdareceptor2bphosphorylationandproducesantinociceptiveeffectsinmicewithinflammatorypain
AT choseongjin inhibitionofspinaltrpv1reducesnmdareceptor2bphosphorylationandproducesantinociceptiveeffectsinmicewithinflammatorypain
AT choikwangho inhibitionofspinaltrpv1reducesnmdareceptor2bphosphorylationandproducesantinociceptiveeffectsinmicewithinflammatorypain
AT ryuyeonhee inhibitionofspinaltrpv1reducesnmdareceptor2bphosphorylationandproducesantinociceptiveeffectsinmicewithinflammatorypain