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Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis

Cryptosporidium bovis is a common enteric pathogen in bovine animals. The research on transmission characteristics of the pathogen is hampered by the lack of subtyping tools. In this study, we retrieve the nucleotide sequence of the 60 kDa glycoprotein (GP60) from the whole genome sequences of C. bo...

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Autores principales: Wang, Weijian, Wan, Muchun, Yang, Fang, Li, Na, Xiao, Lihua, Feng, Yaoyu, Guo, Yaqiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539439/
https://www.ncbi.nlm.nih.gov/pubmed/34683387
http://dx.doi.org/10.3390/microorganisms9102067
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author Wang, Weijian
Wan, Muchun
Yang, Fang
Li, Na
Xiao, Lihua
Feng, Yaoyu
Guo, Yaqiong
author_facet Wang, Weijian
Wan, Muchun
Yang, Fang
Li, Na
Xiao, Lihua
Feng, Yaoyu
Guo, Yaqiong
author_sort Wang, Weijian
collection PubMed
description Cryptosporidium bovis is a common enteric pathogen in bovine animals. The research on transmission characteristics of the pathogen is hampered by the lack of subtyping tools. In this study, we retrieve the nucleotide sequence of the 60 kDa glycoprotein (GP60) from the whole genome sequences of C. bovis we obtained previously and analyze its sequence characteristics. Despite a typical structure of the GP60 protein, the GP60 of C. bovis had only 19.3–45.3% sequence identity to those of other Cryptosporidium species. On the basis of the gene sequence, a subtype typing tool was developed for C. bovis and used in the analysis of 486 C. bovis samples from dairy cattle, yaks, beef cattle, and water buffalos from China. Sixty-eight sequence types were identified from 260 subtyped samples, forming six subtype families, namely XXVIa to XXVIf. The mosaic sequence patterns among subtype families and the 121 potential recombination events identified among the sequences both suggest the occurrence of genetic recombination at the locus. No obvious host adaptation and geographic differences in the distribution of subtype families were observed. Most farms with more extensive sampling had more than one subtype family, and the dominant subtype families on a farm appeared to differ between pre- and post-weaned calves, indicating the likely occurrence of multiple episodes of C. bovis infections. There was an association between XXVId infection and occurrence of moderate diarrhea in dairy cattle. The subtyping tool developed and the data generated in the study might improve our knowledge of the genetic diversity and transmission of C. bovis.
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spelling pubmed-85394392021-10-24 Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis Wang, Weijian Wan, Muchun Yang, Fang Li, Na Xiao, Lihua Feng, Yaoyu Guo, Yaqiong Microorganisms Article Cryptosporidium bovis is a common enteric pathogen in bovine animals. The research on transmission characteristics of the pathogen is hampered by the lack of subtyping tools. In this study, we retrieve the nucleotide sequence of the 60 kDa glycoprotein (GP60) from the whole genome sequences of C. bovis we obtained previously and analyze its sequence characteristics. Despite a typical structure of the GP60 protein, the GP60 of C. bovis had only 19.3–45.3% sequence identity to those of other Cryptosporidium species. On the basis of the gene sequence, a subtype typing tool was developed for C. bovis and used in the analysis of 486 C. bovis samples from dairy cattle, yaks, beef cattle, and water buffalos from China. Sixty-eight sequence types were identified from 260 subtyped samples, forming six subtype families, namely XXVIa to XXVIf. The mosaic sequence patterns among subtype families and the 121 potential recombination events identified among the sequences both suggest the occurrence of genetic recombination at the locus. No obvious host adaptation and geographic differences in the distribution of subtype families were observed. Most farms with more extensive sampling had more than one subtype family, and the dominant subtype families on a farm appeared to differ between pre- and post-weaned calves, indicating the likely occurrence of multiple episodes of C. bovis infections. There was an association between XXVId infection and occurrence of moderate diarrhea in dairy cattle. The subtyping tool developed and the data generated in the study might improve our knowledge of the genetic diversity and transmission of C. bovis. MDPI 2021-09-30 /pmc/articles/PMC8539439/ /pubmed/34683387 http://dx.doi.org/10.3390/microorganisms9102067 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Weijian
Wan, Muchun
Yang, Fang
Li, Na
Xiao, Lihua
Feng, Yaoyu
Guo, Yaqiong
Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis
title Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis
title_full Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis
title_fullStr Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis
title_full_unstemmed Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis
title_short Development and Application of a gp60-Based Subtyping Tool for Cryptosporidium bovis
title_sort development and application of a gp60-based subtyping tool for cryptosporidium bovis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539439/
https://www.ncbi.nlm.nih.gov/pubmed/34683387
http://dx.doi.org/10.3390/microorganisms9102067
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