Cargando…

In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes

The biocompatibility of medical devices, such as implants and prostheses, is strongly determined by the host’s immune response to the implanted material. Monocytes and macrophages are main actors of the so-called foreign body reaction. The innate immune system macrophages (M) can be broadly classifi...

Descripción completa

Detalles Bibliográficos
Autores principales: Woitschach, Franziska, Kloss, Marlen, Schlodder, Karsten, Borck, Alexander, Grabow, Niels, Reisinger, Emil C., Sombetzki, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539455/
https://www.ncbi.nlm.nih.gov/pubmed/34683562
http://dx.doi.org/10.3390/ma14205972
_version_ 1784588751089958912
author Woitschach, Franziska
Kloss, Marlen
Schlodder, Karsten
Borck, Alexander
Grabow, Niels
Reisinger, Emil C.
Sombetzki, Martina
author_facet Woitschach, Franziska
Kloss, Marlen
Schlodder, Karsten
Borck, Alexander
Grabow, Niels
Reisinger, Emil C.
Sombetzki, Martina
author_sort Woitschach, Franziska
collection PubMed
description The biocompatibility of medical devices, such as implants and prostheses, is strongly determined by the host’s immune response to the implanted material. Monocytes and macrophages are main actors of the so-called foreign body reaction. The innate immune system macrophages (M) can be broadly classified into the pro-inflammatory M1-type and the anti-inflammatory, pro-healing M2-type. While a transient inflammatory initial state can be helpful during an infection, persistent inflammation interferes with proper healing and subsequent regeneration. The functional orientation of the immune response, mirrored by monocyte polarization, during interaction with different biomaterials has not yet been sufficiently explored. In implant manufacturing, thermoplastic polyurethane (TPU) represents the state-of-the-art material. The constantly growing areas of application and the associated necessary adaptations make the optimization of these materials indispensable. In the present study, modified liquid silicone rubber (LSR) were compared with two of the most commonly used TPUs, in terms of monocyte adhesion and M1/M2 polarization in vitro. Human monocytes isolated from venous blood were evaluated for their ability to adhere to various biomaterials, their gene expression profile, and their cytokine release. Based on the results, the different polymers exhibit different potential to bias monocytes with respect to early pro-inflammatory cytokine production and gene transcription. Furthermore, none of our test materials showed a clear trend towards M1 or M2 polarization. However, we were able to evaluate the inflammatory potential of the materials, with the classic TPUs appearing to be the most unreactive compared to the silicone-based materials.
format Online
Article
Text
id pubmed-8539455
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85394552021-10-24 In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes Woitschach, Franziska Kloss, Marlen Schlodder, Karsten Borck, Alexander Grabow, Niels Reisinger, Emil C. Sombetzki, Martina Materials (Basel) Article The biocompatibility of medical devices, such as implants and prostheses, is strongly determined by the host’s immune response to the implanted material. Monocytes and macrophages are main actors of the so-called foreign body reaction. The innate immune system macrophages (M) can be broadly classified into the pro-inflammatory M1-type and the anti-inflammatory, pro-healing M2-type. While a transient inflammatory initial state can be helpful during an infection, persistent inflammation interferes with proper healing and subsequent regeneration. The functional orientation of the immune response, mirrored by monocyte polarization, during interaction with different biomaterials has not yet been sufficiently explored. In implant manufacturing, thermoplastic polyurethane (TPU) represents the state-of-the-art material. The constantly growing areas of application and the associated necessary adaptations make the optimization of these materials indispensable. In the present study, modified liquid silicone rubber (LSR) were compared with two of the most commonly used TPUs, in terms of monocyte adhesion and M1/M2 polarization in vitro. Human monocytes isolated from venous blood were evaluated for their ability to adhere to various biomaterials, their gene expression profile, and their cytokine release. Based on the results, the different polymers exhibit different potential to bias monocytes with respect to early pro-inflammatory cytokine production and gene transcription. Furthermore, none of our test materials showed a clear trend towards M1 or M2 polarization. However, we were able to evaluate the inflammatory potential of the materials, with the classic TPUs appearing to be the most unreactive compared to the silicone-based materials. MDPI 2021-10-11 /pmc/articles/PMC8539455/ /pubmed/34683562 http://dx.doi.org/10.3390/ma14205972 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Woitschach, Franziska
Kloss, Marlen
Schlodder, Karsten
Borck, Alexander
Grabow, Niels
Reisinger, Emil C.
Sombetzki, Martina
In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes
title In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes
title_full In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes
title_fullStr In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes
title_full_unstemmed In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes
title_short In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes
title_sort in vitro study of the interaction of innate immune cells with liquid silicone rubber coated with zwitterionic methyl methacrylate and thermoplastic polyurethanes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539455/
https://www.ncbi.nlm.nih.gov/pubmed/34683562
http://dx.doi.org/10.3390/ma14205972
work_keys_str_mv AT woitschachfranziska invitrostudyoftheinteractionofinnateimmunecellswithliquidsiliconerubbercoatedwithzwitterionicmethylmethacrylateandthermoplasticpolyurethanes
AT klossmarlen invitrostudyoftheinteractionofinnateimmunecellswithliquidsiliconerubbercoatedwithzwitterionicmethylmethacrylateandthermoplasticpolyurethanes
AT schlodderkarsten invitrostudyoftheinteractionofinnateimmunecellswithliquidsiliconerubbercoatedwithzwitterionicmethylmethacrylateandthermoplasticpolyurethanes
AT borckalexander invitrostudyoftheinteractionofinnateimmunecellswithliquidsiliconerubbercoatedwithzwitterionicmethylmethacrylateandthermoplasticpolyurethanes
AT grabowniels invitrostudyoftheinteractionofinnateimmunecellswithliquidsiliconerubbercoatedwithzwitterionicmethylmethacrylateandthermoplasticpolyurethanes
AT reisingeremilc invitrostudyoftheinteractionofinnateimmunecellswithliquidsiliconerubbercoatedwithzwitterionicmethylmethacrylateandthermoplasticpolyurethanes
AT sombetzkimartina invitrostudyoftheinteractionofinnateimmunecellswithliquidsiliconerubbercoatedwithzwitterionicmethylmethacrylateandthermoplasticpolyurethanes