Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS

A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer...

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Autores principales: Nikelshparg, Evelina I., Prikhozhdenko, Ekaterina S., Verkhovskii, Roman A., Atkin, Vsevolod S., Khanadeev, Vitaly A., Khlebtsov, Boris N., Bratashov, Daniil N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539561/
https://www.ncbi.nlm.nih.gov/pubmed/34685030
http://dx.doi.org/10.3390/nano11102588
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author Nikelshparg, Evelina I.
Prikhozhdenko, Ekaterina S.
Verkhovskii, Roman A.
Atkin, Vsevolod S.
Khanadeev, Vitaly A.
Khlebtsov, Boris N.
Bratashov, Daniil N.
author_facet Nikelshparg, Evelina I.
Prikhozhdenko, Ekaterina S.
Verkhovskii, Roman A.
Atkin, Vsevolod S.
Khanadeev, Vitaly A.
Khlebtsov, Boris N.
Bratashov, Daniil N.
author_sort Nikelshparg, Evelina I.
collection PubMed
description A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer of poly (4-vinyl pyridine) polymer. This type of SERS substrate provides good cell adhesion and viability. Au nanostars’ long tips can penetrate the cell membrane, allowing it to receive the SERS signal from biomolecules inside a living cell. The proposed nanostructured surfaces were tested to study, label-free, the distribution of various biomolecules in cell compartments.
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spelling pubmed-85395612021-10-24 Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS Nikelshparg, Evelina I. Prikhozhdenko, Ekaterina S. Verkhovskii, Roman A. Atkin, Vsevolod S. Khanadeev, Vitaly A. Khlebtsov, Boris N. Bratashov, Daniil N. Nanomaterials (Basel) Article A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer of poly (4-vinyl pyridine) polymer. This type of SERS substrate provides good cell adhesion and viability. Au nanostars’ long tips can penetrate the cell membrane, allowing it to receive the SERS signal from biomolecules inside a living cell. The proposed nanostructured surfaces were tested to study, label-free, the distribution of various biomolecules in cell compartments. MDPI 2021-09-30 /pmc/articles/PMC8539561/ /pubmed/34685030 http://dx.doi.org/10.3390/nano11102588 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nikelshparg, Evelina I.
Prikhozhdenko, Ekaterina S.
Verkhovskii, Roman A.
Atkin, Vsevolod S.
Khanadeev, Vitaly A.
Khlebtsov, Boris N.
Bratashov, Daniil N.
Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS
title Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS
title_full Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS
title_fullStr Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS
title_full_unstemmed Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS
title_short Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS
title_sort live cell poration by au nanostars to probe intracellular molecular composition with sers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539561/
https://www.ncbi.nlm.nih.gov/pubmed/34685030
http://dx.doi.org/10.3390/nano11102588
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