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 High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression

Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we...

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Autores principales: Vashukova, Elena S., Kozyulina, Polina Y., Illarionov, Roman A., Yurkina, Natalya O., Pachuliia, Olga V., Butenko, Mariya G., Postnikova, Tatyana B., Ivanova, Lada A., Eremeeva, Dina R., Zainulina, Marina S., Bespalova, Olesya N., Glotov, Andrey S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539647/
https://www.ncbi.nlm.nih.gov/pubmed/34685426
http://dx.doi.org/10.3390/life11101055
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author Vashukova, Elena S.
Kozyulina, Polina Y.
Illarionov, Roman A.
Yurkina, Natalya O.
Pachuliia, Olga V.
Butenko, Mariya G.
Postnikova, Tatyana B.
Ivanova, Lada A.
Eremeeva, Dina R.
Zainulina, Marina S.
Bespalova, Olesya N.
Glotov, Andrey S.
author_facet Vashukova, Elena S.
Kozyulina, Polina Y.
Illarionov, Roman A.
Yurkina, Natalya O.
Pachuliia, Olga V.
Butenko, Mariya G.
Postnikova, Tatyana B.
Ivanova, Lada A.
Eremeeva, Dina R.
Zainulina, Marina S.
Bespalova, Olesya N.
Glotov, Andrey S.
author_sort Vashukova, Elena S.
collection PubMed
description Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we evaluated the miRNA profiles in paired plasma and serum samples from pregnant women without health or gestational abnormalities at three time points using high-throughput sequencing technology. Sequencing revealed that the percentage of miRNA reads in plasma and serum decreased by a third compared to first and second trimesters. We found two miRNAs in plasma (hsa-miR-7853-5p and hsa-miR-200c-3p) and 10 miRNAs in serum (hsa-miR-203a-5p, hsa-miR-495-3p, hsa-miR-4435, hsa-miR-340-5p, hsa-miR-4417, hsa-miR-1266-5p, hsa-miR-4494, hsa-miR-134-3p, hsa-miR-5008-5p, and hsa-miR-6756-5p), that exhibit level changes during pregnancy (p-value adjusted < 0.05). In addition, we observed differences for 36 miRNAs between plasma and serum (p-value adjusted < 0.05), which should be taken into consideration when comparing the results between studies performed using different biosample types. The results were verified by analysis of three miRNAs using qRT-PCR (p < 0.05). The present study confirms that the circulating miRNA profile in blood changes during gestation. Our results set the basis for further investigation of molecular mechanisms, involved in regulation of pregnancy, and the search for biomarkers of gestation abnormalities.
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spelling pubmed-85396472021-10-24  High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression Vashukova, Elena S. Kozyulina, Polina Y. Illarionov, Roman A. Yurkina, Natalya O. Pachuliia, Olga V. Butenko, Mariya G. Postnikova, Tatyana B. Ivanova, Lada A. Eremeeva, Dina R. Zainulina, Marina S. Bespalova, Olesya N. Glotov, Andrey S. Life (Basel) Article Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we evaluated the miRNA profiles in paired plasma and serum samples from pregnant women without health or gestational abnormalities at three time points using high-throughput sequencing technology. Sequencing revealed that the percentage of miRNA reads in plasma and serum decreased by a third compared to first and second trimesters. We found two miRNAs in plasma (hsa-miR-7853-5p and hsa-miR-200c-3p) and 10 miRNAs in serum (hsa-miR-203a-5p, hsa-miR-495-3p, hsa-miR-4435, hsa-miR-340-5p, hsa-miR-4417, hsa-miR-1266-5p, hsa-miR-4494, hsa-miR-134-3p, hsa-miR-5008-5p, and hsa-miR-6756-5p), that exhibit level changes during pregnancy (p-value adjusted < 0.05). In addition, we observed differences for 36 miRNAs between plasma and serum (p-value adjusted < 0.05), which should be taken into consideration when comparing the results between studies performed using different biosample types. The results were verified by analysis of three miRNAs using qRT-PCR (p < 0.05). The present study confirms that the circulating miRNA profile in blood changes during gestation. Our results set the basis for further investigation of molecular mechanisms, involved in regulation of pregnancy, and the search for biomarkers of gestation abnormalities. MDPI 2021-10-08 /pmc/articles/PMC8539647/ /pubmed/34685426 http://dx.doi.org/10.3390/life11101055 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vashukova, Elena S.
Kozyulina, Polina Y.
Illarionov, Roman A.
Yurkina, Natalya O.
Pachuliia, Olga V.
Butenko, Mariya G.
Postnikova, Tatyana B.
Ivanova, Lada A.
Eremeeva, Dina R.
Zainulina, Marina S.
Bespalova, Olesya N.
Glotov, Andrey S.
 High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
title  High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
title_full  High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
title_fullStr  High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
title_full_unstemmed  High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
title_short  High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
title_sort  high-throughput sequencing of circulating micrornas in plasma and serum during pregnancy progression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539647/
https://www.ncbi.nlm.nih.gov/pubmed/34685426
http://dx.doi.org/10.3390/life11101055
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