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Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage

Glucosinolates (GLS) are important anionic secondary metabolites that are rich in thiocyanin in cabbage, Brassica oleracea L. var. capitata. GLS are important in food flavor, plant antimicrobial activity, insect resistance, disease resistance, and human anti-cancer effects. Sulfur is an important ra...

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Autores principales: Li, Lushan, Zhang, Hui, Chai, Xiaohong, Wei, Shouhui, Luo, Shilei, Wang, Huiping, Lv, Jian, Yu, Jihua, Liu, Zeci
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539766/
https://www.ncbi.nlm.nih.gov/pubmed/34685913
http://dx.doi.org/10.3390/plants10102104
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author Li, Lushan
Zhang, Hui
Chai, Xiaohong
Wei, Shouhui
Luo, Shilei
Wang, Huiping
Lv, Jian
Yu, Jihua
Liu, Zeci
author_facet Li, Lushan
Zhang, Hui
Chai, Xiaohong
Wei, Shouhui
Luo, Shilei
Wang, Huiping
Lv, Jian
Yu, Jihua
Liu, Zeci
author_sort Li, Lushan
collection PubMed
description Glucosinolates (GLS) are important anionic secondary metabolites that are rich in thiocyanin in cabbage, Brassica oleracea L. var. capitata. GLS are important in food flavor, plant antimicrobial activity, insect resistance, disease resistance, and human anti-cancer effects. Sulfur is an important raw material of GLS, directly affecting their synthesis. However, the mechanism of sulfur regulation of GLS biosynthesis in cabbage is unclear. In the present study, cabbage was treated with sulfur-free Hoagland nutrient solution (control; −S), and normal Hoagland nutrient solution (treatment; +S). Through joint transcriptomic and proteomic analyses, the effect of exogenous S on GLS synthesis was explored. S application induced GLS accumulation; especially, indole glycosides. Transcriptome analysis showed that +S treatment correlated positively with differentially expressed genes and proteins involved in amino acid biosynthesis, carbon metabolism, and plant hormone signal transduction. Compared with −S treatment, the mRNA expression of GLS synthesis genes (CYP, GSTU, UGT, and FMO) and those encoding transcription factors (RLK, MYB, AP2, bHLH, AUX/IAA, and WRKY) were upregulated significantly in the +S group. Combined transcriptome and proteome analysis suggested that the main pathway influenced by S during GLS synthesis in cabbage is amino acid biosynthesis. Moreover, S treatment activated GLS synthesis and accumulation.
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spelling pubmed-85397662021-10-24 Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage Li, Lushan Zhang, Hui Chai, Xiaohong Wei, Shouhui Luo, Shilei Wang, Huiping Lv, Jian Yu, Jihua Liu, Zeci Plants (Basel) Article Glucosinolates (GLS) are important anionic secondary metabolites that are rich in thiocyanin in cabbage, Brassica oleracea L. var. capitata. GLS are important in food flavor, plant antimicrobial activity, insect resistance, disease resistance, and human anti-cancer effects. Sulfur is an important raw material of GLS, directly affecting their synthesis. However, the mechanism of sulfur regulation of GLS biosynthesis in cabbage is unclear. In the present study, cabbage was treated with sulfur-free Hoagland nutrient solution (control; −S), and normal Hoagland nutrient solution (treatment; +S). Through joint transcriptomic and proteomic analyses, the effect of exogenous S on GLS synthesis was explored. S application induced GLS accumulation; especially, indole glycosides. Transcriptome analysis showed that +S treatment correlated positively with differentially expressed genes and proteins involved in amino acid biosynthesis, carbon metabolism, and plant hormone signal transduction. Compared with −S treatment, the mRNA expression of GLS synthesis genes (CYP, GSTU, UGT, and FMO) and those encoding transcription factors (RLK, MYB, AP2, bHLH, AUX/IAA, and WRKY) were upregulated significantly in the +S group. Combined transcriptome and proteome analysis suggested that the main pathway influenced by S during GLS synthesis in cabbage is amino acid biosynthesis. Moreover, S treatment activated GLS synthesis and accumulation. MDPI 2021-10-04 /pmc/articles/PMC8539766/ /pubmed/34685913 http://dx.doi.org/10.3390/plants10102104 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Lushan
Zhang, Hui
Chai, Xiaohong
Wei, Shouhui
Luo, Shilei
Wang, Huiping
Lv, Jian
Yu, Jihua
Liu, Zeci
Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage
title Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage
title_full Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage
title_fullStr Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage
title_full_unstemmed Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage
title_short Transcriptome and Proteome Conjoint Analysis Revealed That Exogenous Sulfur Regulates Glucosinolate Synthesis in Cabbage
title_sort transcriptome and proteome conjoint analysis revealed that exogenous sulfur regulates glucosinolate synthesis in cabbage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539766/
https://www.ncbi.nlm.nih.gov/pubmed/34685913
http://dx.doi.org/10.3390/plants10102104
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