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MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae

Magnaporthe oryzae, a fungal pathogen that causes rice blast, which is the most destructive disease of rice worldwide, has the potential to perform both asexual and sexual reproduction. MAT loci, consisting of MAT genes, were deemed to determine the mating types of M. oryzae strains. However, invest...

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Autores principales: Wang, Jiao-yu, Wang, Shi-zhen, Zhang, Zhen, Hao, Zhong-na, Shi, Xiao-xiao, Li, Ling, Zhu, Xue-ming, Qiu, Hai-ping, Chai, Rong-yao, Wang, Yan-li, Li, Lin, Liu, Xiao-hong, Feng, Xiao-xiao, Sun, Guo-chang, Lin, Fu-cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539793/
https://www.ncbi.nlm.nih.gov/pubmed/34682279
http://dx.doi.org/10.3390/jof7100858
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author Wang, Jiao-yu
Wang, Shi-zhen
Zhang, Zhen
Hao, Zhong-na
Shi, Xiao-xiao
Li, Ling
Zhu, Xue-ming
Qiu, Hai-ping
Chai, Rong-yao
Wang, Yan-li
Li, Lin
Liu, Xiao-hong
Feng, Xiao-xiao
Sun, Guo-chang
Lin, Fu-cheng
author_facet Wang, Jiao-yu
Wang, Shi-zhen
Zhang, Zhen
Hao, Zhong-na
Shi, Xiao-xiao
Li, Ling
Zhu, Xue-ming
Qiu, Hai-ping
Chai, Rong-yao
Wang, Yan-li
Li, Lin
Liu, Xiao-hong
Feng, Xiao-xiao
Sun, Guo-chang
Lin, Fu-cheng
author_sort Wang, Jiao-yu
collection PubMed
description Magnaporthe oryzae, a fungal pathogen that causes rice blast, which is the most destructive disease of rice worldwide, has the potential to perform both asexual and sexual reproduction. MAT loci, consisting of MAT genes, were deemed to determine the mating types of M. oryzae strains. However, investigation was rarely performed on the development and molecular mechanisms of the sexual reproduction of the fungus. In the present work, we analyzed the roles of two MAT loci and five individual MAT genes in the sex determination, sexual development and pathogenicity of M. oryzae. Both of the MAT1-1 and MAT1-2 loci are required for sex determination and the development of sexual structures. MAT1-1-1, MAT1-1-3 and MAT1-2-1 genes are crucial for the formation of perithecium. MAT1-1-2 impacts the generation of asci and ascospores, while MAT1-2-2 is dispensable for sexual development. A GFP fusion experiment indicated that the protein of MAT1-1-3 is distributed in the nucleus. However, all of the MAT loci or MAT genes are dispensable for vegetative growth, asexual reproduction, pathogenicity and pathogenicity-related developments of the fungus, suggesting that sexual reproduction is regulated relatively independently in the development of the fungus. The data and methods of this work may be helpful to further understand the life cycle and the variation of the fungus.
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spelling pubmed-85397932021-10-24 MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae Wang, Jiao-yu Wang, Shi-zhen Zhang, Zhen Hao, Zhong-na Shi, Xiao-xiao Li, Ling Zhu, Xue-ming Qiu, Hai-ping Chai, Rong-yao Wang, Yan-li Li, Lin Liu, Xiao-hong Feng, Xiao-xiao Sun, Guo-chang Lin, Fu-cheng J Fungi (Basel) Article Magnaporthe oryzae, a fungal pathogen that causes rice blast, which is the most destructive disease of rice worldwide, has the potential to perform both asexual and sexual reproduction. MAT loci, consisting of MAT genes, were deemed to determine the mating types of M. oryzae strains. However, investigation was rarely performed on the development and molecular mechanisms of the sexual reproduction of the fungus. In the present work, we analyzed the roles of two MAT loci and five individual MAT genes in the sex determination, sexual development and pathogenicity of M. oryzae. Both of the MAT1-1 and MAT1-2 loci are required for sex determination and the development of sexual structures. MAT1-1-1, MAT1-1-3 and MAT1-2-1 genes are crucial for the formation of perithecium. MAT1-1-2 impacts the generation of asci and ascospores, while MAT1-2-2 is dispensable for sexual development. A GFP fusion experiment indicated that the protein of MAT1-1-3 is distributed in the nucleus. However, all of the MAT loci or MAT genes are dispensable for vegetative growth, asexual reproduction, pathogenicity and pathogenicity-related developments of the fungus, suggesting that sexual reproduction is regulated relatively independently in the development of the fungus. The data and methods of this work may be helpful to further understand the life cycle and the variation of the fungus. MDPI 2021-10-13 /pmc/articles/PMC8539793/ /pubmed/34682279 http://dx.doi.org/10.3390/jof7100858 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jiao-yu
Wang, Shi-zhen
Zhang, Zhen
Hao, Zhong-na
Shi, Xiao-xiao
Li, Ling
Zhu, Xue-ming
Qiu, Hai-ping
Chai, Rong-yao
Wang, Yan-li
Li, Lin
Liu, Xiao-hong
Feng, Xiao-xiao
Sun, Guo-chang
Lin, Fu-cheng
MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae
title MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae
title_full MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae
title_fullStr MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae
title_full_unstemmed MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae
title_short MAT Loci Play Crucial Roles in Sexual Development but Are Dispensable for Asexual Reproduction and Pathogenicity in Rice Blast Fungus Magnaporthe oryzae
title_sort mat loci play crucial roles in sexual development but are dispensable for asexual reproduction and pathogenicity in rice blast fungus magnaporthe oryzae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539793/
https://www.ncbi.nlm.nih.gov/pubmed/34682279
http://dx.doi.org/10.3390/jof7100858
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