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Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae

The widespread genus Cirsium Mill. (Asteraceae) is renowned in traditional medicine. In the present study, an innovative biochemometric-assisted metabolite profiling of the flower heads, aerial parts and roots of Cirsium appendiculatum Griseb. (Balkan thistle) in relation to their antioxidant and en...

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Autores principales: Zheleva-Dimitrova, Dimitrina, Zengin, Gokhan, Ak, Gunes, Sinan, Kouadio Ibrahime, Mahomoodally, Mohamad Fawzi, Gevrenova, Reneta, Balabanova, Vessela, Stefanova, Alexandra, Nedialkov, Paraskev, Voynikov, Yulian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539897/
https://www.ncbi.nlm.nih.gov/pubmed/34685855
http://dx.doi.org/10.3390/plants10102046
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author Zheleva-Dimitrova, Dimitrina
Zengin, Gokhan
Ak, Gunes
Sinan, Kouadio Ibrahime
Mahomoodally, Mohamad Fawzi
Gevrenova, Reneta
Balabanova, Vessela
Stefanova, Alexandra
Nedialkov, Paraskev
Voynikov, Yulian
author_facet Zheleva-Dimitrova, Dimitrina
Zengin, Gokhan
Ak, Gunes
Sinan, Kouadio Ibrahime
Mahomoodally, Mohamad Fawzi
Gevrenova, Reneta
Balabanova, Vessela
Stefanova, Alexandra
Nedialkov, Paraskev
Voynikov, Yulian
author_sort Zheleva-Dimitrova, Dimitrina
collection PubMed
description The widespread genus Cirsium Mill. (Asteraceae) is renowned in traditional medicine. In the present study, an innovative biochemometric-assisted metabolite profiling of the flower heads, aerial parts and roots of Cirsium appendiculatum Griseb. (Balkan thistle) in relation to their antioxidant and enzyme inhibitory potential was developed. The workflow combines ultra-high-performance liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) with partial least-square analysis to discriminate the herbal extracts and identify the most prominent biological activities. The annotation and dereplication of 61 secondary metabolites were evidenced, including 15 carboxylic (including hydroxybenzoic and hydroxycinnamic) acids and their glycosides, 11 acylquinic acids, 26 flavonoids and 9 fatty acids. All compounds were reported for the first time in the studied species. The root extract revealed the highest cupric and ferric reducing power (618.36 ± 5.17 mg TE/g and 269.89 ± 8.50 mg TE/g, respectively) and antioxidant potential in phosphomolybdenum (3.36 ± 0.15 mmol TE/g) as well as the most prominent enzyme inhibitory potential on α-glucosidase (0.72 ± 0.07 mmol ACAE/g), acetylcholinesterase (4.93 ± 0.25 mg GALAE/g) and butyrylcholinesterase (3.80 ± 0.26 mg GALAE/g). Nevertheless, the flower heads were differentiated by their higher metal chelating activity (32.53 ± 3.51 mg EDTAE/g) and total flavonoid content (46.59 ± 0.89 mgRE/g). The partial least-square discriminant and heat-map analysis highlighted the root extract as the most active and a promising source of bioactive compounds for the therapeutic industry.
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spelling pubmed-85398972021-10-24 Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae Zheleva-Dimitrova, Dimitrina Zengin, Gokhan Ak, Gunes Sinan, Kouadio Ibrahime Mahomoodally, Mohamad Fawzi Gevrenova, Reneta Balabanova, Vessela Stefanova, Alexandra Nedialkov, Paraskev Voynikov, Yulian Plants (Basel) Article The widespread genus Cirsium Mill. (Asteraceae) is renowned in traditional medicine. In the present study, an innovative biochemometric-assisted metabolite profiling of the flower heads, aerial parts and roots of Cirsium appendiculatum Griseb. (Balkan thistle) in relation to their antioxidant and enzyme inhibitory potential was developed. The workflow combines ultra-high-performance liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) with partial least-square analysis to discriminate the herbal extracts and identify the most prominent biological activities. The annotation and dereplication of 61 secondary metabolites were evidenced, including 15 carboxylic (including hydroxybenzoic and hydroxycinnamic) acids and their glycosides, 11 acylquinic acids, 26 flavonoids and 9 fatty acids. All compounds were reported for the first time in the studied species. The root extract revealed the highest cupric and ferric reducing power (618.36 ± 5.17 mg TE/g and 269.89 ± 8.50 mg TE/g, respectively) and antioxidant potential in phosphomolybdenum (3.36 ± 0.15 mmol TE/g) as well as the most prominent enzyme inhibitory potential on α-glucosidase (0.72 ± 0.07 mmol ACAE/g), acetylcholinesterase (4.93 ± 0.25 mg GALAE/g) and butyrylcholinesterase (3.80 ± 0.26 mg GALAE/g). Nevertheless, the flower heads were differentiated by their higher metal chelating activity (32.53 ± 3.51 mg EDTAE/g) and total flavonoid content (46.59 ± 0.89 mgRE/g). The partial least-square discriminant and heat-map analysis highlighted the root extract as the most active and a promising source of bioactive compounds for the therapeutic industry. MDPI 2021-09-28 /pmc/articles/PMC8539897/ /pubmed/34685855 http://dx.doi.org/10.3390/plants10102046 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheleva-Dimitrova, Dimitrina
Zengin, Gokhan
Ak, Gunes
Sinan, Kouadio Ibrahime
Mahomoodally, Mohamad Fawzi
Gevrenova, Reneta
Balabanova, Vessela
Stefanova, Alexandra
Nedialkov, Paraskev
Voynikov, Yulian
Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae
title Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae
title_full Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae
title_fullStr Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae
title_full_unstemmed Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae
title_short Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae
title_sort innovative biochemometric approach to the metabolite and biological profiling of the balkan thistle (cirsium appendiculatum griseb.), asteraceae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539897/
https://www.ncbi.nlm.nih.gov/pubmed/34685855
http://dx.doi.org/10.3390/plants10102046
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