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Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats

Melatonin improves metabolic alterations associated with obesity and its diabetes (diabesity). We intend to determine whether this improvement is exerted by changing Zn and/or Cu tissue levels in liver, muscle, pancreas, and brain, and in internal (perirenal, perigonadal, and omentum) and subcutaneo...

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Autores principales: Navarro-Alarcón, Miguel, Gil-Hernández, Fernando, Sánchez-González, Cristina, Llopis, Juan, Villalón-Mir, Marina, Olmedo, Pablo, Alarcón-Guijo, Pablo, Salagre, Diego, Gaona, Lorena, Paredes, Mario, Agil, Ahmad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539996/
https://www.ncbi.nlm.nih.gov/pubmed/34683825
http://dx.doi.org/10.3390/pharmaceutics13101535
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author Navarro-Alarcón, Miguel
Gil-Hernández, Fernando
Sánchez-González, Cristina
Llopis, Juan
Villalón-Mir, Marina
Olmedo, Pablo
Alarcón-Guijo, Pablo
Salagre, Diego
Gaona, Lorena
Paredes, Mario
Agil, Ahmad
author_facet Navarro-Alarcón, Miguel
Gil-Hernández, Fernando
Sánchez-González, Cristina
Llopis, Juan
Villalón-Mir, Marina
Olmedo, Pablo
Alarcón-Guijo, Pablo
Salagre, Diego
Gaona, Lorena
Paredes, Mario
Agil, Ahmad
author_sort Navarro-Alarcón, Miguel
collection PubMed
description Melatonin improves metabolic alterations associated with obesity and its diabetes (diabesity). We intend to determine whether this improvement is exerted by changing Zn and/or Cu tissue levels in liver, muscle, pancreas, and brain, and in internal (perirenal, perigonadal, and omentum) and subcutaneous lumbar white adipose tissues (IWAT and SWAT, respectively). Male Zücker diabetic fatty (ZDF) rats and lean littermates (ZL) were orally supplemented either with melatonin (10 mg/kg body weight/day) or vehicle for 6 weeks. Zn and Cu concentrations were not significantly influenced by diabesity in the analyzed tissues (p > 0.05), with the exception of Zn in liver. In skeletal muscle Zn and Cu, and in perirenal WAT, only Zn levels increased significantly with melatonin supplementation in ZDF rats (p < 0.05). This cytoplasmic Zn enhancement would be probably associated with the upregulation of several Zn influx membrane transporters (Zips) and could explain the amelioration in the glycaemia and insulinaemia by upregulating the Akt and downregulating the inhibitor PTP1B, in obese and diabetic conditions. Enhanced Zn and Cu levels in muscle cells could be related to the reported antioxidant melatonin activity exerted by increasing the Zn, Cu-SOD, and extracellular Cu-SOD activity. In conclusion, melatonin, by increasing the muscle levels of Zn and Cu, joined with our previously reported findings improves glycaemia, insulinaemia, and oxidative stress in this diabesity animal model.
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spelling pubmed-85399962021-10-24 Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats Navarro-Alarcón, Miguel Gil-Hernández, Fernando Sánchez-González, Cristina Llopis, Juan Villalón-Mir, Marina Olmedo, Pablo Alarcón-Guijo, Pablo Salagre, Diego Gaona, Lorena Paredes, Mario Agil, Ahmad Pharmaceutics Article Melatonin improves metabolic alterations associated with obesity and its diabetes (diabesity). We intend to determine whether this improvement is exerted by changing Zn and/or Cu tissue levels in liver, muscle, pancreas, and brain, and in internal (perirenal, perigonadal, and omentum) and subcutaneous lumbar white adipose tissues (IWAT and SWAT, respectively). Male Zücker diabetic fatty (ZDF) rats and lean littermates (ZL) were orally supplemented either with melatonin (10 mg/kg body weight/day) or vehicle for 6 weeks. Zn and Cu concentrations were not significantly influenced by diabesity in the analyzed tissues (p > 0.05), with the exception of Zn in liver. In skeletal muscle Zn and Cu, and in perirenal WAT, only Zn levels increased significantly with melatonin supplementation in ZDF rats (p < 0.05). This cytoplasmic Zn enhancement would be probably associated with the upregulation of several Zn influx membrane transporters (Zips) and could explain the amelioration in the glycaemia and insulinaemia by upregulating the Akt and downregulating the inhibitor PTP1B, in obese and diabetic conditions. Enhanced Zn and Cu levels in muscle cells could be related to the reported antioxidant melatonin activity exerted by increasing the Zn, Cu-SOD, and extracellular Cu-SOD activity. In conclusion, melatonin, by increasing the muscle levels of Zn and Cu, joined with our previously reported findings improves glycaemia, insulinaemia, and oxidative stress in this diabesity animal model. MDPI 2021-09-22 /pmc/articles/PMC8539996/ /pubmed/34683825 http://dx.doi.org/10.3390/pharmaceutics13101535 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Navarro-Alarcón, Miguel
Gil-Hernández, Fernando
Sánchez-González, Cristina
Llopis, Juan
Villalón-Mir, Marina
Olmedo, Pablo
Alarcón-Guijo, Pablo
Salagre, Diego
Gaona, Lorena
Paredes, Mario
Agil, Ahmad
Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats
title Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats
title_full Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats
title_fullStr Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats
title_full_unstemmed Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats
title_short Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats
title_sort melatonin improves levels of zn and cu in the muscle of diabetic obese rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539996/
https://www.ncbi.nlm.nih.gov/pubmed/34683825
http://dx.doi.org/10.3390/pharmaceutics13101535
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