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Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats
Melatonin improves metabolic alterations associated with obesity and its diabetes (diabesity). We intend to determine whether this improvement is exerted by changing Zn and/or Cu tissue levels in liver, muscle, pancreas, and brain, and in internal (perirenal, perigonadal, and omentum) and subcutaneo...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539996/ https://www.ncbi.nlm.nih.gov/pubmed/34683825 http://dx.doi.org/10.3390/pharmaceutics13101535 |
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author | Navarro-Alarcón, Miguel Gil-Hernández, Fernando Sánchez-González, Cristina Llopis, Juan Villalón-Mir, Marina Olmedo, Pablo Alarcón-Guijo, Pablo Salagre, Diego Gaona, Lorena Paredes, Mario Agil, Ahmad |
author_facet | Navarro-Alarcón, Miguel Gil-Hernández, Fernando Sánchez-González, Cristina Llopis, Juan Villalón-Mir, Marina Olmedo, Pablo Alarcón-Guijo, Pablo Salagre, Diego Gaona, Lorena Paredes, Mario Agil, Ahmad |
author_sort | Navarro-Alarcón, Miguel |
collection | PubMed |
description | Melatonin improves metabolic alterations associated with obesity and its diabetes (diabesity). We intend to determine whether this improvement is exerted by changing Zn and/or Cu tissue levels in liver, muscle, pancreas, and brain, and in internal (perirenal, perigonadal, and omentum) and subcutaneous lumbar white adipose tissues (IWAT and SWAT, respectively). Male Zücker diabetic fatty (ZDF) rats and lean littermates (ZL) were orally supplemented either with melatonin (10 mg/kg body weight/day) or vehicle for 6 weeks. Zn and Cu concentrations were not significantly influenced by diabesity in the analyzed tissues (p > 0.05), with the exception of Zn in liver. In skeletal muscle Zn and Cu, and in perirenal WAT, only Zn levels increased significantly with melatonin supplementation in ZDF rats (p < 0.05). This cytoplasmic Zn enhancement would be probably associated with the upregulation of several Zn influx membrane transporters (Zips) and could explain the amelioration in the glycaemia and insulinaemia by upregulating the Akt and downregulating the inhibitor PTP1B, in obese and diabetic conditions. Enhanced Zn and Cu levels in muscle cells could be related to the reported antioxidant melatonin activity exerted by increasing the Zn, Cu-SOD, and extracellular Cu-SOD activity. In conclusion, melatonin, by increasing the muscle levels of Zn and Cu, joined with our previously reported findings improves glycaemia, insulinaemia, and oxidative stress in this diabesity animal model. |
format | Online Article Text |
id | pubmed-8539996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85399962021-10-24 Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats Navarro-Alarcón, Miguel Gil-Hernández, Fernando Sánchez-González, Cristina Llopis, Juan Villalón-Mir, Marina Olmedo, Pablo Alarcón-Guijo, Pablo Salagre, Diego Gaona, Lorena Paredes, Mario Agil, Ahmad Pharmaceutics Article Melatonin improves metabolic alterations associated with obesity and its diabetes (diabesity). We intend to determine whether this improvement is exerted by changing Zn and/or Cu tissue levels in liver, muscle, pancreas, and brain, and in internal (perirenal, perigonadal, and omentum) and subcutaneous lumbar white adipose tissues (IWAT and SWAT, respectively). Male Zücker diabetic fatty (ZDF) rats and lean littermates (ZL) were orally supplemented either with melatonin (10 mg/kg body weight/day) or vehicle for 6 weeks. Zn and Cu concentrations were not significantly influenced by diabesity in the analyzed tissues (p > 0.05), with the exception of Zn in liver. In skeletal muscle Zn and Cu, and in perirenal WAT, only Zn levels increased significantly with melatonin supplementation in ZDF rats (p < 0.05). This cytoplasmic Zn enhancement would be probably associated with the upregulation of several Zn influx membrane transporters (Zips) and could explain the amelioration in the glycaemia and insulinaemia by upregulating the Akt and downregulating the inhibitor PTP1B, in obese and diabetic conditions. Enhanced Zn and Cu levels in muscle cells could be related to the reported antioxidant melatonin activity exerted by increasing the Zn, Cu-SOD, and extracellular Cu-SOD activity. In conclusion, melatonin, by increasing the muscle levels of Zn and Cu, joined with our previously reported findings improves glycaemia, insulinaemia, and oxidative stress in this diabesity animal model. MDPI 2021-09-22 /pmc/articles/PMC8539996/ /pubmed/34683825 http://dx.doi.org/10.3390/pharmaceutics13101535 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Navarro-Alarcón, Miguel Gil-Hernández, Fernando Sánchez-González, Cristina Llopis, Juan Villalón-Mir, Marina Olmedo, Pablo Alarcón-Guijo, Pablo Salagre, Diego Gaona, Lorena Paredes, Mario Agil, Ahmad Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats |
title | Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats |
title_full | Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats |
title_fullStr | Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats |
title_full_unstemmed | Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats |
title_short | Melatonin Improves Levels of Zn and Cu in the Muscle of Diabetic Obese Rats |
title_sort | melatonin improves levels of zn and cu in the muscle of diabetic obese rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539996/ https://www.ncbi.nlm.nih.gov/pubmed/34683825 http://dx.doi.org/10.3390/pharmaceutics13101535 |
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