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Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca

Salmonella Heidelberg (SH) on contaminated poultry causes economic and health risks to producers and consumers. We hypothesized that sodium bisulfate (SBS) would decrease SH biofilm on polyvinyl chloride (PVC) coupons and decrease the horizontal transfer of SH in broilers. Experiment 1: Salmonella H...

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Autores principales: Pineda, Megan Rose, Byrd, James Allen, Genovese, Kenneth James, Farnell, Yuhua Zhang, Zhao, Dan, Wang, Xi, Milby, Allison Claire, Farnell, Morgan Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540007/
https://www.ncbi.nlm.nih.gov/pubmed/34683366
http://dx.doi.org/10.3390/microorganisms9102047
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author Pineda, Megan Rose
Byrd, James Allen
Genovese, Kenneth James
Farnell, Yuhua Zhang
Zhao, Dan
Wang, Xi
Milby, Allison Claire
Farnell, Morgan Brian
author_facet Pineda, Megan Rose
Byrd, James Allen
Genovese, Kenneth James
Farnell, Yuhua Zhang
Zhao, Dan
Wang, Xi
Milby, Allison Claire
Farnell, Morgan Brian
author_sort Pineda, Megan Rose
collection PubMed
description Salmonella Heidelberg (SH) on contaminated poultry causes economic and health risks to producers and consumers. We hypothesized that sodium bisulfate (SBS) would decrease SH biofilm on polyvinyl chloride (PVC) coupons and decrease the horizontal transfer of SH in broilers. Experiment 1: Salmonella Heidelberg biofilm was cultured with PVC coupons, which were treated with SBS at a pH of 3.5 for 10 min, 8 h, and 24 h. Experiment 2: Nine replicate pens per treatment were divided between two rooms. A seeder contact model was used to mimic a natural infection environment. Treatments consisted of tap water or sodium bisulfate in water at a pH of 3.5. Salmonella Heidelberg incidence and enumeration were measured in crops and ceca. Sodium bisulfate significantly reduced biofilm by 2.16 and 1.04 logs when treated for 8 and 24 h, respectively. Crop colonization was significantly decreased in trials 1 and 2 by 0.29 and 0.23 logs, respectively. Crop pH was significantly decreased in trial 2. Ceca colonization was significantly decreased in trial 1 by 0.39 logs. The results from the present study suggest that SBS may be administered to drinking water to decrease SH gut colonization and to reduce biofilm.
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spelling pubmed-85400072021-10-24 Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca Pineda, Megan Rose Byrd, James Allen Genovese, Kenneth James Farnell, Yuhua Zhang Zhao, Dan Wang, Xi Milby, Allison Claire Farnell, Morgan Brian Microorganisms Article Salmonella Heidelberg (SH) on contaminated poultry causes economic and health risks to producers and consumers. We hypothesized that sodium bisulfate (SBS) would decrease SH biofilm on polyvinyl chloride (PVC) coupons and decrease the horizontal transfer of SH in broilers. Experiment 1: Salmonella Heidelberg biofilm was cultured with PVC coupons, which were treated with SBS at a pH of 3.5 for 10 min, 8 h, and 24 h. Experiment 2: Nine replicate pens per treatment were divided between two rooms. A seeder contact model was used to mimic a natural infection environment. Treatments consisted of tap water or sodium bisulfate in water at a pH of 3.5. Salmonella Heidelberg incidence and enumeration were measured in crops and ceca. Sodium bisulfate significantly reduced biofilm by 2.16 and 1.04 logs when treated for 8 and 24 h, respectively. Crop colonization was significantly decreased in trials 1 and 2 by 0.29 and 0.23 logs, respectively. Crop pH was significantly decreased in trial 2. Ceca colonization was significantly decreased in trial 1 by 0.39 logs. The results from the present study suggest that SBS may be administered to drinking water to decrease SH gut colonization and to reduce biofilm. MDPI 2021-09-28 /pmc/articles/PMC8540007/ /pubmed/34683366 http://dx.doi.org/10.3390/microorganisms9102047 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pineda, Megan Rose
Byrd, James Allen
Genovese, Kenneth James
Farnell, Yuhua Zhang
Zhao, Dan
Wang, Xi
Milby, Allison Claire
Farnell, Morgan Brian
Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca
title Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca
title_full Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca
title_fullStr Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca
title_full_unstemmed Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca
title_short Evaluation of Sodium Bisulfate on Reducing Salmonella Heidelberg Biofilm and Colonization in Broiler Crops and Ceca
title_sort evaluation of sodium bisulfate on reducing salmonella heidelberg biofilm and colonization in broiler crops and ceca
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540007/
https://www.ncbi.nlm.nih.gov/pubmed/34683366
http://dx.doi.org/10.3390/microorganisms9102047
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