Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082

LC-MS/MS-based molecular networking facilitated the targeted isolation of a new cyclic hexadepsipeptide, cymodepsipeptide (1), and two known analogues, RF–2691A (2) and RF–2691B (3), from the fungus Cymostachys sp. NBUF082 that was derived from a mesophotic zone Aaptos sponge collected near Apo Isla...

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Autores principales: Yuan, Ye, Li, Te, Wang, Tingting, Naman, C. Benjamin, Ye, Jing, Wu, Xingxin, Lazaro, J. Enrico H., Yan, Xiaojun, He, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540034/
https://www.ncbi.nlm.nih.gov/pubmed/34677465
http://dx.doi.org/10.3390/md19100565
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author Yuan, Ye
Li, Te
Wang, Tingting
Naman, C. Benjamin
Ye, Jing
Wu, Xingxin
Lazaro, J. Enrico H.
Yan, Xiaojun
He, Shan
author_facet Yuan, Ye
Li, Te
Wang, Tingting
Naman, C. Benjamin
Ye, Jing
Wu, Xingxin
Lazaro, J. Enrico H.
Yan, Xiaojun
He, Shan
author_sort Yuan, Ye
collection PubMed
description LC-MS/MS-based molecular networking facilitated the targeted isolation of a new cyclic hexadepsipeptide, cymodepsipeptide (1), and two known analogues, RF–2691A (2) and RF–2691B (3), from the fungus Cymostachys sp. NBUF082 that was derived from a mesophotic zone Aaptos sponge collected near Apo Island. The constitution and configuration of 1 was elucidated through 1D and 2D NMR-spectroscopy, high resolution mass-spectrometry, and chemical degradations including Marfey’s analysis and chiral HPLC. It was observed that 1 was moderately cytotoxic against CCRF-CEM human acute lymphocytic leukemia cells in vitro with the IC(50) value of 9.2 ± 1.1 μM.
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spelling pubmed-85400342021-10-24 Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082 Yuan, Ye Li, Te Wang, Tingting Naman, C. Benjamin Ye, Jing Wu, Xingxin Lazaro, J. Enrico H. Yan, Xiaojun He, Shan Mar Drugs Communication LC-MS/MS-based molecular networking facilitated the targeted isolation of a new cyclic hexadepsipeptide, cymodepsipeptide (1), and two known analogues, RF–2691A (2) and RF–2691B (3), from the fungus Cymostachys sp. NBUF082 that was derived from a mesophotic zone Aaptos sponge collected near Apo Island. The constitution and configuration of 1 was elucidated through 1D and 2D NMR-spectroscopy, high resolution mass-spectrometry, and chemical degradations including Marfey’s analysis and chiral HPLC. It was observed that 1 was moderately cytotoxic against CCRF-CEM human acute lymphocytic leukemia cells in vitro with the IC(50) value of 9.2 ± 1.1 μM. MDPI 2021-10-11 /pmc/articles/PMC8540034/ /pubmed/34677465 http://dx.doi.org/10.3390/md19100565 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Yuan, Ye
Li, Te
Wang, Tingting
Naman, C. Benjamin
Ye, Jing
Wu, Xingxin
Lazaro, J. Enrico H.
Yan, Xiaojun
He, Shan
Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082
title Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082
title_full Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082
title_fullStr Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082
title_full_unstemmed Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082
title_short Targeted Isolation of a Cytotoxic Cyclic Hexadepsipeptide from the Mesophotic Zone Sponge-Associated Fungus Cymostachys sp. NBUF082
title_sort targeted isolation of a cytotoxic cyclic hexadepsipeptide from the mesophotic zone sponge-associated fungus cymostachys sp. nbuf082
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540034/
https://www.ncbi.nlm.nih.gov/pubmed/34677465
http://dx.doi.org/10.3390/md19100565
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