Cargando…

Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)

Collagen filaments derived from the two marine demosponges Ircinia oros and Sarcotragus foetidus were for the first time isolated, biochemically characterised and tested for their potential use in regenerative medicine. SDS-PAGE of isolated filaments revealed a main collagen subunit band of 130 kDa...

Descripción completa

Detalles Bibliográficos
Autores principales: Pozzolini, Marina, Tassara, Eleonora, Dodero, Andrea, Castellano, Maila, Vicini, Silvia, Ferrando, Sara, Aicardi, Stefano, Cavallo, Dario, Bertolino, Marco, Petrenko, Iaroslav, Ehrlich, Hermann, Giovine, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540060/
https://www.ncbi.nlm.nih.gov/pubmed/34677462
http://dx.doi.org/10.3390/md19100563
_version_ 1784588896299909120
author Pozzolini, Marina
Tassara, Eleonora
Dodero, Andrea
Castellano, Maila
Vicini, Silvia
Ferrando, Sara
Aicardi, Stefano
Cavallo, Dario
Bertolino, Marco
Petrenko, Iaroslav
Ehrlich, Hermann
Giovine, Marco
author_facet Pozzolini, Marina
Tassara, Eleonora
Dodero, Andrea
Castellano, Maila
Vicini, Silvia
Ferrando, Sara
Aicardi, Stefano
Cavallo, Dario
Bertolino, Marco
Petrenko, Iaroslav
Ehrlich, Hermann
Giovine, Marco
author_sort Pozzolini, Marina
collection PubMed
description Collagen filaments derived from the two marine demosponges Ircinia oros and Sarcotragus foetidus were for the first time isolated, biochemically characterised and tested for their potential use in regenerative medicine. SDS-PAGE of isolated filaments revealed a main collagen subunit band of 130 kDa in both of the samples under study. DSC analysis on 2D membranes produced with collagenous sponge filaments showed higher thermal stability than commercial mammalian-derived collagen membranes. Dynamic mechanical and thermal analysis attested that the membranes obtained from filaments of S. foetidus were more resistant and stable at the rising temperature, compared to the ones derived from filaments of I. oros. Moreover, the former has higher stability in saline and in collagenase solutions and evident antioxidant activity. Conversely, their water binding capacity results were lower than that of membranes obtained from I. oros. Adhesion and proliferation tests using L929 fibroblasts and HaCaT keratinocytes resulted in a remarkable biocompatibility of both developed membrane models, and gene expression analysis showed an evident up-regulation of ECM-related genes. Finally, membranes from I. oros significantly increased type I collagen gene expression and its release in the culture medium. The findings here reported strongly suggest the biotechnological potential of these collagenous structures of poriferan origin as scaffolds for wound healing.
format Online
Article
Text
id pubmed-8540060
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85400602021-10-24 Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862) Pozzolini, Marina Tassara, Eleonora Dodero, Andrea Castellano, Maila Vicini, Silvia Ferrando, Sara Aicardi, Stefano Cavallo, Dario Bertolino, Marco Petrenko, Iaroslav Ehrlich, Hermann Giovine, Marco Mar Drugs Article Collagen filaments derived from the two marine demosponges Ircinia oros and Sarcotragus foetidus were for the first time isolated, biochemically characterised and tested for their potential use in regenerative medicine. SDS-PAGE of isolated filaments revealed a main collagen subunit band of 130 kDa in both of the samples under study. DSC analysis on 2D membranes produced with collagenous sponge filaments showed higher thermal stability than commercial mammalian-derived collagen membranes. Dynamic mechanical and thermal analysis attested that the membranes obtained from filaments of S. foetidus were more resistant and stable at the rising temperature, compared to the ones derived from filaments of I. oros. Moreover, the former has higher stability in saline and in collagenase solutions and evident antioxidant activity. Conversely, their water binding capacity results were lower than that of membranes obtained from I. oros. Adhesion and proliferation tests using L929 fibroblasts and HaCaT keratinocytes resulted in a remarkable biocompatibility of both developed membrane models, and gene expression analysis showed an evident up-regulation of ECM-related genes. Finally, membranes from I. oros significantly increased type I collagen gene expression and its release in the culture medium. The findings here reported strongly suggest the biotechnological potential of these collagenous structures of poriferan origin as scaffolds for wound healing. MDPI 2021-10-06 /pmc/articles/PMC8540060/ /pubmed/34677462 http://dx.doi.org/10.3390/md19100563 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pozzolini, Marina
Tassara, Eleonora
Dodero, Andrea
Castellano, Maila
Vicini, Silvia
Ferrando, Sara
Aicardi, Stefano
Cavallo, Dario
Bertolino, Marco
Petrenko, Iaroslav
Ehrlich, Hermann
Giovine, Marco
Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)
title Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)
title_full Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)
title_fullStr Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)
title_full_unstemmed Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)
title_short Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)
title_sort potential biomedical applications of collagen filaments derived from the marine demosponges ircinia oros (schmidt, 1864) and sarcotragus foetidus (schmidt, 1862)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540060/
https://www.ncbi.nlm.nih.gov/pubmed/34677462
http://dx.doi.org/10.3390/md19100563
work_keys_str_mv AT pozzolinimarina potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT tassaraeleonora potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT doderoandrea potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT castellanomaila potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT vicinisilvia potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT ferrandosara potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT aicardistefano potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT cavallodario potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT bertolinomarco potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT petrenkoiaroslav potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT ehrlichhermann potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862
AT giovinemarco potentialbiomedicalapplicationsofcollagenfilamentsderivedfromthemarinedemospongesirciniaorosschmidt1864andsarcotragusfoetidusschmidt1862