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Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples

Zearalenone (ZEA) is a secondary metabolite produced by Fusarium spp., the filamentous fungi. Food and feed contamination with zearalenone has adverse effects on health and economy. ZEA degradation through microorganisms is providing a promising preventive measure. The current study includes isolati...

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Autores principales: Zada, Sharif, Alam, Sadia, Ayoubi, Samha Al, Shakeela, Qismat, Nisa, Sobia, Niaz, Zeeshan, Khan, Ibrar, Ahmed, Waqas, Bibi, Yamin, Ahmed, Shehzad, Qayyum, Abdul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540131/
https://www.ncbi.nlm.nih.gov/pubmed/34679005
http://dx.doi.org/10.3390/toxins13100712
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author Zada, Sharif
Alam, Sadia
Ayoubi, Samha Al
Shakeela, Qismat
Nisa, Sobia
Niaz, Zeeshan
Khan, Ibrar
Ahmed, Waqas
Bibi, Yamin
Ahmed, Shehzad
Qayyum, Abdul
author_facet Zada, Sharif
Alam, Sadia
Ayoubi, Samha Al
Shakeela, Qismat
Nisa, Sobia
Niaz, Zeeshan
Khan, Ibrar
Ahmed, Waqas
Bibi, Yamin
Ahmed, Shehzad
Qayyum, Abdul
author_sort Zada, Sharif
collection PubMed
description Zearalenone (ZEA) is a secondary metabolite produced by Fusarium spp., the filamentous fungi. Food and feed contamination with zearalenone has adverse effects on health and economy. ZEA degradation through microorganisms is providing a promising preventive measure. The current study includes isolation of 47 bacterial strains from 100 different food and rumen samples. Seventeen isolates showed maximum activity of ZEA reduction. A bacterial isolate, RS-5, reduced ZEA concentration up to 78.3% through ELISA analysis and 74.3% as determined through HPLC. Ten of the most efficient strains were further selected for comparison of their biodegradation activity in different conditions such as incubation period, and different growth media. The samples were analyzed after 24 h, 48 h, and 72 h of incubation. De Man Rogosa Sharp (MRS) broth, Tryptic soy broth, and nutrient broth were used as different carbon sources for comparison of activity through ELISA. The mean degradation % ± SD through ELISA and HPLC were 70.77% ± 3.935 and 69.11% ± 2.768, respectively. Optimum reducing activity was detected at 72 h of incubation, and MRS broth is a suitable medium. Phylogenetic analysis based on 16S rRNA gene nucleotide sequences confirmed that one of the bacterial isolate RS-5 bacterial isolates with higher mycotoxin degradation is identified as Bacillus subtilis isolated from rumen sample. B05 (FSL-8) bacterial isolate of yogurt belongs to the genus Lactobacillus with 99.66% similarity with Lactobacillus delbrukii. Similarly, three other bacterial isolates, D05, H05 and F04 (FS-17, FSL-2 and FS-20), were found to be the sub-species/strains Pseudomonas gessardii of genus Pseudomonas based on their similarity level of (99.2%, 96% and 96.88%) and positioning in the phylogenetic tree. Promising detoxification results were revealed through GC-MS analysis of RS-5 and FSL-8 activity.
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spelling pubmed-85401312021-10-24 Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples Zada, Sharif Alam, Sadia Ayoubi, Samha Al Shakeela, Qismat Nisa, Sobia Niaz, Zeeshan Khan, Ibrar Ahmed, Waqas Bibi, Yamin Ahmed, Shehzad Qayyum, Abdul Toxins (Basel) Article Zearalenone (ZEA) is a secondary metabolite produced by Fusarium spp., the filamentous fungi. Food and feed contamination with zearalenone has adverse effects on health and economy. ZEA degradation through microorganisms is providing a promising preventive measure. The current study includes isolation of 47 bacterial strains from 100 different food and rumen samples. Seventeen isolates showed maximum activity of ZEA reduction. A bacterial isolate, RS-5, reduced ZEA concentration up to 78.3% through ELISA analysis and 74.3% as determined through HPLC. Ten of the most efficient strains were further selected for comparison of their biodegradation activity in different conditions such as incubation period, and different growth media. The samples were analyzed after 24 h, 48 h, and 72 h of incubation. De Man Rogosa Sharp (MRS) broth, Tryptic soy broth, and nutrient broth were used as different carbon sources for comparison of activity through ELISA. The mean degradation % ± SD through ELISA and HPLC were 70.77% ± 3.935 and 69.11% ± 2.768, respectively. Optimum reducing activity was detected at 72 h of incubation, and MRS broth is a suitable medium. Phylogenetic analysis based on 16S rRNA gene nucleotide sequences confirmed that one of the bacterial isolate RS-5 bacterial isolates with higher mycotoxin degradation is identified as Bacillus subtilis isolated from rumen sample. B05 (FSL-8) bacterial isolate of yogurt belongs to the genus Lactobacillus with 99.66% similarity with Lactobacillus delbrukii. Similarly, three other bacterial isolates, D05, H05 and F04 (FS-17, FSL-2 and FS-20), were found to be the sub-species/strains Pseudomonas gessardii of genus Pseudomonas based on their similarity level of (99.2%, 96% and 96.88%) and positioning in the phylogenetic tree. Promising detoxification results were revealed through GC-MS analysis of RS-5 and FSL-8 activity. MDPI 2021-10-09 /pmc/articles/PMC8540131/ /pubmed/34679005 http://dx.doi.org/10.3390/toxins13100712 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zada, Sharif
Alam, Sadia
Ayoubi, Samha Al
Shakeela, Qismat
Nisa, Sobia
Niaz, Zeeshan
Khan, Ibrar
Ahmed, Waqas
Bibi, Yamin
Ahmed, Shehzad
Qayyum, Abdul
Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples
title Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples
title_full Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples
title_fullStr Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples
title_full_unstemmed Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples
title_short Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples
title_sort biological transformation of zearalenone by some bacterial isolates associated with ruminant and food samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540131/
https://www.ncbi.nlm.nih.gov/pubmed/34679005
http://dx.doi.org/10.3390/toxins13100712
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