Cargando…

Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep

Teladorsagia circumcincta is the most important gastrointestinal parasite in the livestock industry in temperate regions around the world, causing great economic losses. The infective third-stage larvae (L3) of Teladorsagia circumcincta secrete a large number of excretory-secretory (E/S) molecules,...

Descripción completa

Detalles Bibliográficos
Autores principales: Hafidi, Nur Nasuha, Swan, Jaclyn, Faou, Pierre, Lowe, Rohan, Rajapaksha, Harinda, Cairns, Callum, Stear, Michael, Beddoe, Travis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540209/
https://www.ncbi.nlm.nih.gov/pubmed/34679046
http://dx.doi.org/10.3390/vetsci8100216
_version_ 1784588931560374272
author Hafidi, Nur Nasuha
Swan, Jaclyn
Faou, Pierre
Lowe, Rohan
Rajapaksha, Harinda
Cairns, Callum
Stear, Michael
Beddoe, Travis
author_facet Hafidi, Nur Nasuha
Swan, Jaclyn
Faou, Pierre
Lowe, Rohan
Rajapaksha, Harinda
Cairns, Callum
Stear, Michael
Beddoe, Travis
author_sort Hafidi, Nur Nasuha
collection PubMed
description Teladorsagia circumcincta is the most important gastrointestinal parasite in the livestock industry in temperate regions around the world, causing great economic losses. The infective third-stage larvae (L3) of Teladorsagia circumcincta secrete a large number of excretory-secretory (E/S) molecules, some of which are likely to play critical roles in modulating the host immune response. One of the most abundant E/S molecules is a protein termed Tci-gal-1, which has similarity to mammalian galectins. Galectins are a family of carbohydrate-binding molecules, with characteristic domain organisation and affinity for β-galactosids that mediate a variety of important cellular functions including inflammation and immune responses. To understand the role of Tci-gal-1 at the host–parasite interface, we used a proteomics pull-down approach to identify Tc-gal-1 interacting proteins from sheep abomasal scrapes and whole tissue. A total of 135 unique proteins were identified from whole abomasal tissue samples, while 89 proteins were isolated from abomasal scrape samples. Of these proteins, 63 were present in both samples. Many of the host proteins identified, such as trefoil factors and mucin-like proteins, play critical roles in the host response. The identification of Tci-gal-1 binding partners provides new insights on host–parasite interactions and could lead to the development of new control strategies.
format Online
Article
Text
id pubmed-8540209
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85402092021-10-24 Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep Hafidi, Nur Nasuha Swan, Jaclyn Faou, Pierre Lowe, Rohan Rajapaksha, Harinda Cairns, Callum Stear, Michael Beddoe, Travis Vet Sci Article Teladorsagia circumcincta is the most important gastrointestinal parasite in the livestock industry in temperate regions around the world, causing great economic losses. The infective third-stage larvae (L3) of Teladorsagia circumcincta secrete a large number of excretory-secretory (E/S) molecules, some of which are likely to play critical roles in modulating the host immune response. One of the most abundant E/S molecules is a protein termed Tci-gal-1, which has similarity to mammalian galectins. Galectins are a family of carbohydrate-binding molecules, with characteristic domain organisation and affinity for β-galactosids that mediate a variety of important cellular functions including inflammation and immune responses. To understand the role of Tci-gal-1 at the host–parasite interface, we used a proteomics pull-down approach to identify Tc-gal-1 interacting proteins from sheep abomasal scrapes and whole tissue. A total of 135 unique proteins were identified from whole abomasal tissue samples, while 89 proteins were isolated from abomasal scrape samples. Of these proteins, 63 were present in both samples. Many of the host proteins identified, such as trefoil factors and mucin-like proteins, play critical roles in the host response. The identification of Tci-gal-1 binding partners provides new insights on host–parasite interactions and could lead to the development of new control strategies. MDPI 2021-10-04 /pmc/articles/PMC8540209/ /pubmed/34679046 http://dx.doi.org/10.3390/vetsci8100216 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hafidi, Nur Nasuha
Swan, Jaclyn
Faou, Pierre
Lowe, Rohan
Rajapaksha, Harinda
Cairns, Callum
Stear, Michael
Beddoe, Travis
Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep
title Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep
title_full Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep
title_fullStr Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep
title_full_unstemmed Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep
title_short Teladorsagia Circumcincta Galectin-Mucosal Interactome in Sheep
title_sort teladorsagia circumcincta galectin-mucosal interactome in sheep
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540209/
https://www.ncbi.nlm.nih.gov/pubmed/34679046
http://dx.doi.org/10.3390/vetsci8100216
work_keys_str_mv AT hafidinurnasuha teladorsagiacircumcinctagalectinmucosalinteractomeinsheep
AT swanjaclyn teladorsagiacircumcinctagalectinmucosalinteractomeinsheep
AT faoupierre teladorsagiacircumcinctagalectinmucosalinteractomeinsheep
AT lowerohan teladorsagiacircumcinctagalectinmucosalinteractomeinsheep
AT rajapakshaharinda teladorsagiacircumcinctagalectinmucosalinteractomeinsheep
AT cairnscallum teladorsagiacircumcinctagalectinmucosalinteractomeinsheep
AT stearmichael teladorsagiacircumcinctagalectinmucosalinteractomeinsheep
AT beddoetravis teladorsagiacircumcinctagalectinmucosalinteractomeinsheep