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BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells
The 2016 Zika virus (ZIKV) epidemic illustrates the impact of flaviviruses as emerging human pathogens. For unknown reasons, ZIKV replicates more efficiently in neural progenitor cells (NPCs) than in postmitotic neurons. Here, we identified host factors used by ZIKV using the NCI-60 library of cell...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540262/ https://www.ncbi.nlm.nih.gov/pubmed/34696437 http://dx.doi.org/10.3390/v13102007 |
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author | Persson, B. David Nord, Stefan Lindqvist, Richard Danskog, Katarina Överby, Anna K. Kohl, Alain Willison, Hugh J. Lenman, Annasara Arnberg, Niklas |
author_facet | Persson, B. David Nord, Stefan Lindqvist, Richard Danskog, Katarina Överby, Anna K. Kohl, Alain Willison, Hugh J. Lenman, Annasara Arnberg, Niklas |
author_sort | Persson, B. David |
collection | PubMed |
description | The 2016 Zika virus (ZIKV) epidemic illustrates the impact of flaviviruses as emerging human pathogens. For unknown reasons, ZIKV replicates more efficiently in neural progenitor cells (NPCs) than in postmitotic neurons. Here, we identified host factors used by ZIKV using the NCI-60 library of cell lines and COMPARE analysis, and cross-analyzed this library with two other libraries of host factors with importance for ZIKV infection. We identified BAF45b, a subunit of the BAF (Brg1/Brm-associated factors) protein complexes that regulate differentiation of NPCs to post-mitotic neurons. ZIKV (and other flaviviruses) infected HAP1 cells deficient in expression of BAF45b and other BAF subunits less efficiently than wildtype (WT) HAP1 cells. We concluded that subunits of the BAF complex are important for infection of ZIKV and other flavivirus. Given their function in cell and tissue differentiation, such regulators may be important determinants of tropism and pathogenesis of arthropod-borne flaviviruses. |
format | Online Article Text |
id | pubmed-8540262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85402622021-10-24 BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells Persson, B. David Nord, Stefan Lindqvist, Richard Danskog, Katarina Överby, Anna K. Kohl, Alain Willison, Hugh J. Lenman, Annasara Arnberg, Niklas Viruses Communication The 2016 Zika virus (ZIKV) epidemic illustrates the impact of flaviviruses as emerging human pathogens. For unknown reasons, ZIKV replicates more efficiently in neural progenitor cells (NPCs) than in postmitotic neurons. Here, we identified host factors used by ZIKV using the NCI-60 library of cell lines and COMPARE analysis, and cross-analyzed this library with two other libraries of host factors with importance for ZIKV infection. We identified BAF45b, a subunit of the BAF (Brg1/Brm-associated factors) protein complexes that regulate differentiation of NPCs to post-mitotic neurons. ZIKV (and other flaviviruses) infected HAP1 cells deficient in expression of BAF45b and other BAF subunits less efficiently than wildtype (WT) HAP1 cells. We concluded that subunits of the BAF complex are important for infection of ZIKV and other flavivirus. Given their function in cell and tissue differentiation, such regulators may be important determinants of tropism and pathogenesis of arthropod-borne flaviviruses. MDPI 2021-10-06 /pmc/articles/PMC8540262/ /pubmed/34696437 http://dx.doi.org/10.3390/v13102007 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Persson, B. David Nord, Stefan Lindqvist, Richard Danskog, Katarina Överby, Anna K. Kohl, Alain Willison, Hugh J. Lenman, Annasara Arnberg, Niklas BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells |
title | BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells |
title_full | BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells |
title_fullStr | BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells |
title_full_unstemmed | BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells |
title_short | BAF45b Is Required for Efficient Zika Virus Infection of HAP1 Cells |
title_sort | baf45b is required for efficient zika virus infection of hap1 cells |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540262/ https://www.ncbi.nlm.nih.gov/pubmed/34696437 http://dx.doi.org/10.3390/v13102007 |
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