Cargando…

Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry

Cucumis melo L is one of the most commercial and economical crops in the world with several health beneficial compounds as such carotenoids, amino acids, vitamin A and C, minerals, and dietary fiber. Evaluation of the volatile organic compounds (VOCs) in different melon (Cucumis melo L.) breeding li...

Descripción completa

Detalles Bibliográficos
Autores principales: Majithia, Drishti, Metrani, Rita, Dhowlaghar, Nitin, Crosby, Kevin M., Patil, Bhimanagouda S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540282/
https://www.ncbi.nlm.nih.gov/pubmed/34685975
http://dx.doi.org/10.3390/plants10102166
_version_ 1784588949112487936
author Majithia, Drishti
Metrani, Rita
Dhowlaghar, Nitin
Crosby, Kevin M.
Patil, Bhimanagouda S.
author_facet Majithia, Drishti
Metrani, Rita
Dhowlaghar, Nitin
Crosby, Kevin M.
Patil, Bhimanagouda S.
author_sort Majithia, Drishti
collection PubMed
description Cucumis melo L is one of the most commercial and economical crops in the world with several health beneficial compounds as such carotenoids, amino acids, vitamin A and C, minerals, and dietary fiber. Evaluation of the volatile organic compounds (VOCs) in different melon (Cucumis melo L.) breeding lines provides useful information for improving fruit flavor, aroma, and antimicrobial levels. In this study, the VOCs in 28 melon breeding lines harvested in 2019 were identified and characterized using head space solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This identified 113 VOCs with significant differences in composition and contents of among the breeding lines, including 15 esters, 27 aldehydes, 35 alcohols, 14 ketones, 4 acids, 10 hydrocarbons, 5 sulfurs, and 3 other compounds. The highest average contents of all the VOCs were found in BL-30 (13,973.07 µg/kg FW) and the lowest were in BL-22 (3947.13 µg/kg FW). BL-9 had high levels of carotenoid-derived VOCs. The compounds with the highest contents were benzaldehyde, geranylacetone, and β-ionone. Quality parameters such as color and sugar contents of melons were also measured. All the melon color readings were within the typical acceptable range. BL-22 and BL-14 had the highest and lowest sugar contents, respectively. Principal component analysis (PCA) produced diverse clusters of breeding lines based on flavor and aroma. BL-4, BL-7, BL-12, BL-20, and BL-30 were thus selected as important breeding lines based on their organoleptic, antimicrobial, and health-beneficial properties.
format Online
Article
Text
id pubmed-8540282
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85402822021-10-24 Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry Majithia, Drishti Metrani, Rita Dhowlaghar, Nitin Crosby, Kevin M. Patil, Bhimanagouda S. Plants (Basel) Article Cucumis melo L is one of the most commercial and economical crops in the world with several health beneficial compounds as such carotenoids, amino acids, vitamin A and C, minerals, and dietary fiber. Evaluation of the volatile organic compounds (VOCs) in different melon (Cucumis melo L.) breeding lines provides useful information for improving fruit flavor, aroma, and antimicrobial levels. In this study, the VOCs in 28 melon breeding lines harvested in 2019 were identified and characterized using head space solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This identified 113 VOCs with significant differences in composition and contents of among the breeding lines, including 15 esters, 27 aldehydes, 35 alcohols, 14 ketones, 4 acids, 10 hydrocarbons, 5 sulfurs, and 3 other compounds. The highest average contents of all the VOCs were found in BL-30 (13,973.07 µg/kg FW) and the lowest were in BL-22 (3947.13 µg/kg FW). BL-9 had high levels of carotenoid-derived VOCs. The compounds with the highest contents were benzaldehyde, geranylacetone, and β-ionone. Quality parameters such as color and sugar contents of melons were also measured. All the melon color readings were within the typical acceptable range. BL-22 and BL-14 had the highest and lowest sugar contents, respectively. Principal component analysis (PCA) produced diverse clusters of breeding lines based on flavor and aroma. BL-4, BL-7, BL-12, BL-20, and BL-30 were thus selected as important breeding lines based on their organoleptic, antimicrobial, and health-beneficial properties. MDPI 2021-10-13 /pmc/articles/PMC8540282/ /pubmed/34685975 http://dx.doi.org/10.3390/plants10102166 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Majithia, Drishti
Metrani, Rita
Dhowlaghar, Nitin
Crosby, Kevin M.
Patil, Bhimanagouda S.
Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
title Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
title_full Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
title_fullStr Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
title_full_unstemmed Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
title_short Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
title_sort assessment and classification of volatile profiles in melon breeding lines using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540282/
https://www.ncbi.nlm.nih.gov/pubmed/34685975
http://dx.doi.org/10.3390/plants10102166
work_keys_str_mv AT majithiadrishti assessmentandclassificationofvolatileprofilesinmelonbreedinglinesusingheadspacesolidphasemicroextractioncoupledwithgaschromatographymassspectrometry
AT metranirita assessmentandclassificationofvolatileprofilesinmelonbreedinglinesusingheadspacesolidphasemicroextractioncoupledwithgaschromatographymassspectrometry
AT dhowlagharnitin assessmentandclassificationofvolatileprofilesinmelonbreedinglinesusingheadspacesolidphasemicroextractioncoupledwithgaschromatographymassspectrometry
AT crosbykevinm assessmentandclassificationofvolatileprofilesinmelonbreedinglinesusingheadspacesolidphasemicroextractioncoupledwithgaschromatographymassspectrometry
AT patilbhimanagoudas assessmentandclassificationofvolatileprofilesinmelonbreedinglinesusingheadspacesolidphasemicroextractioncoupledwithgaschromatographymassspectrometry