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CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences
RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consis...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540305/ https://www.ncbi.nlm.nih.gov/pubmed/34685863 http://dx.doi.org/10.3390/plants10102055 |
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author | Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin |
author_facet | Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin |
author_sort | Karlson, Chou Khai Soong |
collection | PubMed |
description | RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement. |
format | Online Article Text |
id | pubmed-8540305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85403052021-10-24 CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin Plants (Basel) Review RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement. MDPI 2021-09-29 /pmc/articles/PMC8540305/ /pubmed/34685863 http://dx.doi.org/10.3390/plants10102055 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences |
title | CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences |
title_full | CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences |
title_fullStr | CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences |
title_full_unstemmed | CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences |
title_short | CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences |
title_sort | crispr/dcas9-based systems: mechanisms and applications in plant sciences |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540305/ https://www.ncbi.nlm.nih.gov/pubmed/34685863 http://dx.doi.org/10.3390/plants10102055 |
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