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Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production
We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography and deep reactive ion etching. However, a challenge remains...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540318/ https://www.ncbi.nlm.nih.gov/pubmed/34683304 http://dx.doi.org/10.3390/mi12101253 |
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author | Kumon, Hiroki Sakuma, Shinya Nakamura, Sou Maruyama, Hisataka Eto, Koji Arai, Fumihito |
author_facet | Kumon, Hiroki Sakuma, Shinya Nakamura, Sou Maruyama, Hisataka Eto, Koji Arai, Fumihito |
author_sort | Kumon, Hiroki |
collection | PubMed |
description | We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography and deep reactive ion etching. However, a challenge remains in observing the detailed process of PLT production owing to the low visibility of the microfluidic bioreactor. In this paper, we present a transparent microfluidic bioreactor made of cyclo-olefin polymer (COP) with which to observe the process of platelet-like particle (PLP) production under a bright-field, which allows us to obtain image data at a high sampling rate. We succeeded in fabricating the COP microfluidic bioreactor with a 3D microchannel. We investigated the bonding strength of COP-COP layers and confirmed the effectiveness of the microfluidic bioreactor. Results of on-chip PLP production using immortalized megakaryocyte cell lines (imMKCLs) derived from human-induced pluripotent stem cells show that the average total number of produced PLPs per imMKCL was 17.6 PLPs/imMKCL, which is comparable to that of our previous glass–Si–glass microfluidic bioreactor (17.4 PLPs/imMKCL). We succeeded in observing PLP production under a bright-field using the presented microfluidic bioreactor and confirmed that PLP fragmented in a narrow area of proplatelet-like protrusions. |
format | Online Article Text |
id | pubmed-8540318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85403182021-10-24 Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production Kumon, Hiroki Sakuma, Shinya Nakamura, Sou Maruyama, Hisataka Eto, Koji Arai, Fumihito Micromachines (Basel) Article We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography and deep reactive ion etching. However, a challenge remains in observing the detailed process of PLT production owing to the low visibility of the microfluidic bioreactor. In this paper, we present a transparent microfluidic bioreactor made of cyclo-olefin polymer (COP) with which to observe the process of platelet-like particle (PLP) production under a bright-field, which allows us to obtain image data at a high sampling rate. We succeeded in fabricating the COP microfluidic bioreactor with a 3D microchannel. We investigated the bonding strength of COP-COP layers and confirmed the effectiveness of the microfluidic bioreactor. Results of on-chip PLP production using immortalized megakaryocyte cell lines (imMKCLs) derived from human-induced pluripotent stem cells show that the average total number of produced PLPs per imMKCL was 17.6 PLPs/imMKCL, which is comparable to that of our previous glass–Si–glass microfluidic bioreactor (17.4 PLPs/imMKCL). We succeeded in observing PLP production under a bright-field using the presented microfluidic bioreactor and confirmed that PLP fragmented in a narrow area of proplatelet-like protrusions. MDPI 2021-10-15 /pmc/articles/PMC8540318/ /pubmed/34683304 http://dx.doi.org/10.3390/mi12101253 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kumon, Hiroki Sakuma, Shinya Nakamura, Sou Maruyama, Hisataka Eto, Koji Arai, Fumihito Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production |
title | Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production |
title_full | Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production |
title_fullStr | Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production |
title_full_unstemmed | Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production |
title_short | Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production |
title_sort | microfluidic bioreactor made of cyclo-olefin polymer for observing on-chip platelet production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540318/ https://www.ncbi.nlm.nih.gov/pubmed/34683304 http://dx.doi.org/10.3390/mi12101253 |
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