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Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production

We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography and deep reactive ion etching. However, a challenge remains...

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Autores principales: Kumon, Hiroki, Sakuma, Shinya, Nakamura, Sou, Maruyama, Hisataka, Eto, Koji, Arai, Fumihito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540318/
https://www.ncbi.nlm.nih.gov/pubmed/34683304
http://dx.doi.org/10.3390/mi12101253
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author Kumon, Hiroki
Sakuma, Shinya
Nakamura, Sou
Maruyama, Hisataka
Eto, Koji
Arai, Fumihito
author_facet Kumon, Hiroki
Sakuma, Shinya
Nakamura, Sou
Maruyama, Hisataka
Eto, Koji
Arai, Fumihito
author_sort Kumon, Hiroki
collection PubMed
description We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography and deep reactive ion etching. However, a challenge remains in observing the detailed process of PLT production owing to the low visibility of the microfluidic bioreactor. In this paper, we present a transparent microfluidic bioreactor made of cyclo-olefin polymer (COP) with which to observe the process of platelet-like particle (PLP) production under a bright-field, which allows us to obtain image data at a high sampling rate. We succeeded in fabricating the COP microfluidic bioreactor with a 3D microchannel. We investigated the bonding strength of COP-COP layers and confirmed the effectiveness of the microfluidic bioreactor. Results of on-chip PLP production using immortalized megakaryocyte cell lines (imMKCLs) derived from human-induced pluripotent stem cells show that the average total number of produced PLPs per imMKCL was 17.6 PLPs/imMKCL, which is comparable to that of our previous glass–Si–glass microfluidic bioreactor (17.4 PLPs/imMKCL). We succeeded in observing PLP production under a bright-field using the presented microfluidic bioreactor and confirmed that PLP fragmented in a narrow area of proplatelet-like protrusions.
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spelling pubmed-85403182021-10-24 Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production Kumon, Hiroki Sakuma, Shinya Nakamura, Sou Maruyama, Hisataka Eto, Koji Arai, Fumihito Micromachines (Basel) Article We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography and deep reactive ion etching. However, a challenge remains in observing the detailed process of PLT production owing to the low visibility of the microfluidic bioreactor. In this paper, we present a transparent microfluidic bioreactor made of cyclo-olefin polymer (COP) with which to observe the process of platelet-like particle (PLP) production under a bright-field, which allows us to obtain image data at a high sampling rate. We succeeded in fabricating the COP microfluidic bioreactor with a 3D microchannel. We investigated the bonding strength of COP-COP layers and confirmed the effectiveness of the microfluidic bioreactor. Results of on-chip PLP production using immortalized megakaryocyte cell lines (imMKCLs) derived from human-induced pluripotent stem cells show that the average total number of produced PLPs per imMKCL was 17.6 PLPs/imMKCL, which is comparable to that of our previous glass–Si–glass microfluidic bioreactor (17.4 PLPs/imMKCL). We succeeded in observing PLP production under a bright-field using the presented microfluidic bioreactor and confirmed that PLP fragmented in a narrow area of proplatelet-like protrusions. MDPI 2021-10-15 /pmc/articles/PMC8540318/ /pubmed/34683304 http://dx.doi.org/10.3390/mi12101253 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kumon, Hiroki
Sakuma, Shinya
Nakamura, Sou
Maruyama, Hisataka
Eto, Koji
Arai, Fumihito
Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production
title Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production
title_full Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production
title_fullStr Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production
title_full_unstemmed Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production
title_short Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production
title_sort microfluidic bioreactor made of cyclo-olefin polymer for observing on-chip platelet production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540318/
https://www.ncbi.nlm.nih.gov/pubmed/34683304
http://dx.doi.org/10.3390/mi12101253
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