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Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people
BACKGROUND: Interferon gamma release assays (IGRAs) are used to detect latent Mycobacterium tuberculosis (M.tb) infection (LTBI) in adults, but their performance in older people is not well-established. We evaluated IGRAs for LTBI detection in older Hispanic recent TB contacts (ReC) or community con...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540394/ https://www.ncbi.nlm.nih.gov/pubmed/34389503 http://dx.doi.org/10.1016/j.ijid.2021.08.014 |
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author | Scordo, Julia M. Aguillón-Durán, Génesis P. Ayala, Doris Quirino-Cerrillo, Ana Paulina Rodríguez-Reyna, Eminé Joya-Ayala, Mateo Mora-Guzmán, Francisco Ledezma-Campos, Eder Villafañez, Alejandro Schlesinger, Larry S. Torrelles, Jordi B. Turner, Joanne Restrepo, Blanca I. |
author_facet | Scordo, Julia M. Aguillón-Durán, Génesis P. Ayala, Doris Quirino-Cerrillo, Ana Paulina Rodríguez-Reyna, Eminé Joya-Ayala, Mateo Mora-Guzmán, Francisco Ledezma-Campos, Eder Villafañez, Alejandro Schlesinger, Larry S. Torrelles, Jordi B. Turner, Joanne Restrepo, Blanca I. |
author_sort | Scordo, Julia M. |
collection | PubMed |
description | BACKGROUND: Interferon gamma release assays (IGRAs) are used to detect latent Mycobacterium tuberculosis (M.tb) infection (LTBI) in adults, but their performance in older people is not well-established. We evaluated IGRAs for LTBI detection in older Hispanic recent TB contacts (ReC) or community controls (CoC). METHODS: Cross-sectional assessment of LTBI with T-SPOT.TB and/or QuantiFERON-Gold in-tube or –Plus assay in older (≥60 years) and adult (18–50 years) Hispanic people. RESULTS: We enrolled 193 CoC (119 adults, 74 older persons) and 459 ReC (361 adults, 98 older persons). LTBI positivity increased with age in CoC (19%–59%, P<0.001), but was similar in ReC (59%–69%, P=0.329). Older people had lower concordance between IGRAs (kappa 0.465 vs 0.688 in adults) and more inconclusive results (indeterminate/borderline 11.6% vs 5.8% in adults, P=0.012). With simultaneous IGRAs, inconclusive results were resolved as positive or negative with the other IGRA. The magnitude of response to M.tb peptides in IGRAs was similar among age groups, but responsiveness to mitogens was lower in older people. CONCLUSIONS: IGRAs are suitable for LTBI detection in older people. Discordant and inconclusive findings are more prevalent in older people, but results are resolved when IGRA is repeated with a different IGRA test. |
format | Online Article Text |
id | pubmed-8540394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-85403942021-10-23 Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people Scordo, Julia M. Aguillón-Durán, Génesis P. Ayala, Doris Quirino-Cerrillo, Ana Paulina Rodríguez-Reyna, Eminé Joya-Ayala, Mateo Mora-Guzmán, Francisco Ledezma-Campos, Eder Villafañez, Alejandro Schlesinger, Larry S. Torrelles, Jordi B. Turner, Joanne Restrepo, Blanca I. Int J Infect Dis Article BACKGROUND: Interferon gamma release assays (IGRAs) are used to detect latent Mycobacterium tuberculosis (M.tb) infection (LTBI) in adults, but their performance in older people is not well-established. We evaluated IGRAs for LTBI detection in older Hispanic recent TB contacts (ReC) or community controls (CoC). METHODS: Cross-sectional assessment of LTBI with T-SPOT.TB and/or QuantiFERON-Gold in-tube or –Plus assay in older (≥60 years) and adult (18–50 years) Hispanic people. RESULTS: We enrolled 193 CoC (119 adults, 74 older persons) and 459 ReC (361 adults, 98 older persons). LTBI positivity increased with age in CoC (19%–59%, P<0.001), but was similar in ReC (59%–69%, P=0.329). Older people had lower concordance between IGRAs (kappa 0.465 vs 0.688 in adults) and more inconclusive results (indeterminate/borderline 11.6% vs 5.8% in adults, P=0.012). With simultaneous IGRAs, inconclusive results were resolved as positive or negative with the other IGRA. The magnitude of response to M.tb peptides in IGRAs was similar among age groups, but responsiveness to mitogens was lower in older people. CONCLUSIONS: IGRAs are suitable for LTBI detection in older people. Discordant and inconclusive findings are more prevalent in older people, but results are resolved when IGRA is repeated with a different IGRA test. 2021-08-10 2021-10 /pmc/articles/PMC8540394/ /pubmed/34389503 http://dx.doi.org/10.1016/j.ijid.2021.08.014 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Article Scordo, Julia M. Aguillón-Durán, Génesis P. Ayala, Doris Quirino-Cerrillo, Ana Paulina Rodríguez-Reyna, Eminé Joya-Ayala, Mateo Mora-Guzmán, Francisco Ledezma-Campos, Eder Villafañez, Alejandro Schlesinger, Larry S. Torrelles, Jordi B. Turner, Joanne Restrepo, Blanca I. Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people |
title | Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people |
title_full | Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people |
title_fullStr | Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people |
title_full_unstemmed | Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people |
title_short | Interferon gamma release assays for detection of latent Mycobacterium tuberculosis in older Hispanic people |
title_sort | interferon gamma release assays for detection of latent mycobacterium tuberculosis in older hispanic people |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540394/ https://www.ncbi.nlm.nih.gov/pubmed/34389503 http://dx.doi.org/10.1016/j.ijid.2021.08.014 |
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