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Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications

Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-l...

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Autores principales: Sciabica, Sabrina, Tafuro, Giovanni, Semenzato, Alessandra, Traini, Daniela, Silva, Dina M., Reis, Larissa Gomes Dos, Canilli, Luisa, Terno, Massimo, Durini, Elisa, Vertuani, Silvia, Baldisserotto, Anna, Manfredini, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540713/
https://www.ncbi.nlm.nih.gov/pubmed/34683965
http://dx.doi.org/10.3390/pharmaceutics13101672
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author Sciabica, Sabrina
Tafuro, Giovanni
Semenzato, Alessandra
Traini, Daniela
Silva, Dina M.
Reis, Larissa Gomes Dos
Canilli, Luisa
Terno, Massimo
Durini, Elisa
Vertuani, Silvia
Baldisserotto, Anna
Manfredini, Stefano
author_facet Sciabica, Sabrina
Tafuro, Giovanni
Semenzato, Alessandra
Traini, Daniela
Silva, Dina M.
Reis, Larissa Gomes Dos
Canilli, Luisa
Terno, Massimo
Durini, Elisa
Vertuani, Silvia
Baldisserotto, Anna
Manfredini, Stefano
author_sort Sciabica, Sabrina
collection PubMed
description Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-life and consequently increase performance, native HA has been modified through cross-linking reactions with a natural and biocompatible amino acid, Ornithine, to overcome the potential toxicity commonly associated with traditional linkers. 2-chloro-dimethoxy-1,3,5-triazine/4-methylmorpholine (CDMT/NMM) was used as an activating agent. The new product (HA–Orn) was extensively characterized to confirm the chemical modification, and rheological analysis showed a gel-like profile. In vitro degradation experiments showed an improved resistance profile against enzymatic digestions. Furthermore, in vitro cytotoxicity studies were performed on lung cell lines (Calu-3 and H441), which showed no cytotoxicity.
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spelling pubmed-85407132021-10-24 Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications Sciabica, Sabrina Tafuro, Giovanni Semenzato, Alessandra Traini, Daniela Silva, Dina M. Reis, Larissa Gomes Dos Canilli, Luisa Terno, Massimo Durini, Elisa Vertuani, Silvia Baldisserotto, Anna Manfredini, Stefano Pharmaceutics Article Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-life and consequently increase performance, native HA has been modified through cross-linking reactions with a natural and biocompatible amino acid, Ornithine, to overcome the potential toxicity commonly associated with traditional linkers. 2-chloro-dimethoxy-1,3,5-triazine/4-methylmorpholine (CDMT/NMM) was used as an activating agent. The new product (HA–Orn) was extensively characterized to confirm the chemical modification, and rheological analysis showed a gel-like profile. In vitro degradation experiments showed an improved resistance profile against enzymatic digestions. Furthermore, in vitro cytotoxicity studies were performed on lung cell lines (Calu-3 and H441), which showed no cytotoxicity. MDPI 2021-10-13 /pmc/articles/PMC8540713/ /pubmed/34683965 http://dx.doi.org/10.3390/pharmaceutics13101672 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sciabica, Sabrina
Tafuro, Giovanni
Semenzato, Alessandra
Traini, Daniela
Silva, Dina M.
Reis, Larissa Gomes Dos
Canilli, Luisa
Terno, Massimo
Durini, Elisa
Vertuani, Silvia
Baldisserotto, Anna
Manfredini, Stefano
Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
title Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
title_full Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
title_fullStr Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
title_full_unstemmed Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
title_short Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
title_sort design, synthesis, characterization, and in vitro evaluation of a new cross-linked hyaluronic acid for pharmaceutical and cosmetic applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540713/
https://www.ncbi.nlm.nih.gov/pubmed/34683965
http://dx.doi.org/10.3390/pharmaceutics13101672
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