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Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5

Crown rust, caused by Puccinia coronata f. sp. avenae, is one of the most destructive fungal diseases of oat worldwide. Growing disease-resistant oat cultivars is the preferred method of preventing the spread of rust and potential epidemics. The object of the study was Pc50-5, a race-specific seedli...

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Autores principales: Toporowska, Joanna, Sowa, Sylwia, Kilian, Andrzej, Koroluk, Aneta, Paczos-Grzęda, Edyta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540790/
https://www.ncbi.nlm.nih.gov/pubmed/34681841
http://dx.doi.org/10.3390/ijms222011183
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author Toporowska, Joanna
Sowa, Sylwia
Kilian, Andrzej
Koroluk, Aneta
Paczos-Grzęda, Edyta
author_facet Toporowska, Joanna
Sowa, Sylwia
Kilian, Andrzej
Koroluk, Aneta
Paczos-Grzęda, Edyta
author_sort Toporowska, Joanna
collection PubMed
description Crown rust, caused by Puccinia coronata f. sp. avenae, is one of the most destructive fungal diseases of oat worldwide. Growing disease-resistant oat cultivars is the preferred method of preventing the spread of rust and potential epidemics. The object of the study was Pc50-5, a race-specific seedling crown rust resistant gene, highly effective at all growth stages, selected from the differential line Pc50 (Avena sterilis L. CW 486-1 × Pendek). A comparison of crown rust reaction as well as an allelism test showed the distinctiveness of Pc50-5, whereas the proportions of phenotypes in segregating populations derived from a cross with two crown rust-susceptible Polish oat cultivars, Kasztan × Pc50-5 and Bingo × Pc50-5, confirmed monogenic inheritance of the gene, indicating its usefulness in oat breeding programs. Effective gene introgression depends on reliable gene identification in the early stages of plant development; thus, the aim of the study was to develop molecular markers that are tightly linked to Pc50-5. Segregating populations of Kasztan × Pc50-5 were genotyped using DArTseq technology based on next-generation Illumina short-read sequencing. Markers associated with Pc50-5 were located on chromosome 6A of the current version of the oat reference genome (Avena sativa OT3098 v2, PepsiCo) in the region between 434,234,214 and 440,149,046 bp and subsequently converted to PCR-based SCAR (sequence-characterized amplified region) markers. Furthermore, 5426978_SCAR and 24031809_SCAR co-segregated with the Pc50-5 resistance allele and were mapped to the partial linkage group at 0.6 and 4.0 cM, respectively. The co-dominant 58163643_SCAR marker was the best diagnostic and it was located closest to Pc50-5 at 0.1 cM. The newly discovered, very strong monogenic crown rust resistance may be useful for oat improvement. DArTseq sequences converted into specific PCR markers will be a valuable tool for marker-assisted selection in breeding programs.
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spelling pubmed-85407902021-10-24 Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5 Toporowska, Joanna Sowa, Sylwia Kilian, Andrzej Koroluk, Aneta Paczos-Grzęda, Edyta Int J Mol Sci Article Crown rust, caused by Puccinia coronata f. sp. avenae, is one of the most destructive fungal diseases of oat worldwide. Growing disease-resistant oat cultivars is the preferred method of preventing the spread of rust and potential epidemics. The object of the study was Pc50-5, a race-specific seedling crown rust resistant gene, highly effective at all growth stages, selected from the differential line Pc50 (Avena sterilis L. CW 486-1 × Pendek). A comparison of crown rust reaction as well as an allelism test showed the distinctiveness of Pc50-5, whereas the proportions of phenotypes in segregating populations derived from a cross with two crown rust-susceptible Polish oat cultivars, Kasztan × Pc50-5 and Bingo × Pc50-5, confirmed monogenic inheritance of the gene, indicating its usefulness in oat breeding programs. Effective gene introgression depends on reliable gene identification in the early stages of plant development; thus, the aim of the study was to develop molecular markers that are tightly linked to Pc50-5. Segregating populations of Kasztan × Pc50-5 were genotyped using DArTseq technology based on next-generation Illumina short-read sequencing. Markers associated with Pc50-5 were located on chromosome 6A of the current version of the oat reference genome (Avena sativa OT3098 v2, PepsiCo) in the region between 434,234,214 and 440,149,046 bp and subsequently converted to PCR-based SCAR (sequence-characterized amplified region) markers. Furthermore, 5426978_SCAR and 24031809_SCAR co-segregated with the Pc50-5 resistance allele and were mapped to the partial linkage group at 0.6 and 4.0 cM, respectively. The co-dominant 58163643_SCAR marker was the best diagnostic and it was located closest to Pc50-5 at 0.1 cM. The newly discovered, very strong monogenic crown rust resistance may be useful for oat improvement. DArTseq sequences converted into specific PCR markers will be a valuable tool for marker-assisted selection in breeding programs. MDPI 2021-10-17 /pmc/articles/PMC8540790/ /pubmed/34681841 http://dx.doi.org/10.3390/ijms222011183 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Toporowska, Joanna
Sowa, Sylwia
Kilian, Andrzej
Koroluk, Aneta
Paczos-Grzęda, Edyta
Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5
title Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5
title_full Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5
title_fullStr Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5
title_full_unstemmed Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5
title_short Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5
title_sort discovery and chromosomal location a highly effective oat crown rust resistance gene pc50-5
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540790/
https://www.ncbi.nlm.nih.gov/pubmed/34681841
http://dx.doi.org/10.3390/ijms222011183
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