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Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021

The first detection of a Highly Pathogenic Avian Influenza (HPAI) H5N8 virus in Bulgaria dates back to December 2016. Since then, many outbreaks caused by HPAI H5 viruses from clade 2.3.4.4B have been reported in both domestic and wild birds in different regions of the country. In this study, we cha...

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Autores principales: Zecchin, Bianca, Goujgoulova, Gabriela, Monne, Isabella, Salviato, Annalisa, Schivo, Alessia, Slavcheva, Iskra, Pastori, Ambra, Brown, Ian H., Lewis, Nicola S., Terregino, Calogero, Fusaro, Alice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541051/
https://www.ncbi.nlm.nih.gov/pubmed/34696516
http://dx.doi.org/10.3390/v13102086
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author Zecchin, Bianca
Goujgoulova, Gabriela
Monne, Isabella
Salviato, Annalisa
Schivo, Alessia
Slavcheva, Iskra
Pastori, Ambra
Brown, Ian H.
Lewis, Nicola S.
Terregino, Calogero
Fusaro, Alice
author_facet Zecchin, Bianca
Goujgoulova, Gabriela
Monne, Isabella
Salviato, Annalisa
Schivo, Alessia
Slavcheva, Iskra
Pastori, Ambra
Brown, Ian H.
Lewis, Nicola S.
Terregino, Calogero
Fusaro, Alice
author_sort Zecchin, Bianca
collection PubMed
description The first detection of a Highly Pathogenic Avian Influenza (HPAI) H5N8 virus in Bulgaria dates back to December 2016. Since then, many outbreaks caused by HPAI H5 viruses from clade 2.3.4.4B have been reported in both domestic and wild birds in different regions of the country. In this study, we characterized the complete genome of sixteen H5 viruses collected in Bulgaria between 2019 and 2021. Phylogenetic analyses revealed a persistent circulation of the H5N8 strain for four consecutive years (December 2016–June 2020) and the emergence in 2020 of a novel reassortant H5N2 subtype, likely in a duck farm. Estimation of the time to the most recent common ancestor indicates that this reassortment event may have occurred between May 2019 and January 2020. At the beginning of 2021, Bulgaria experienced a new virus introduction in the poultry sector, namely a HPAI H5N8 that had been circulating in Europe since October 2020. The periodical identification in domestic birds of H5 viruses related to the 2016 epidemic as well as a reassortant strain might indicate undetected circulation of the virus in resident wild birds or in the poultry sector. To avoid the concealed circulation and evolution of viruses, and the risk of emergence of strains with pandemic potential, the implementation of control measures is of utmost importance, particularly in duck farms where birds display no clinical signs.
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spelling pubmed-85410512021-10-24 Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021 Zecchin, Bianca Goujgoulova, Gabriela Monne, Isabella Salviato, Annalisa Schivo, Alessia Slavcheva, Iskra Pastori, Ambra Brown, Ian H. Lewis, Nicola S. Terregino, Calogero Fusaro, Alice Viruses Article The first detection of a Highly Pathogenic Avian Influenza (HPAI) H5N8 virus in Bulgaria dates back to December 2016. Since then, many outbreaks caused by HPAI H5 viruses from clade 2.3.4.4B have been reported in both domestic and wild birds in different regions of the country. In this study, we characterized the complete genome of sixteen H5 viruses collected in Bulgaria between 2019 and 2021. Phylogenetic analyses revealed a persistent circulation of the H5N8 strain for four consecutive years (December 2016–June 2020) and the emergence in 2020 of a novel reassortant H5N2 subtype, likely in a duck farm. Estimation of the time to the most recent common ancestor indicates that this reassortment event may have occurred between May 2019 and January 2020. At the beginning of 2021, Bulgaria experienced a new virus introduction in the poultry sector, namely a HPAI H5N8 that had been circulating in Europe since October 2020. The periodical identification in domestic birds of H5 viruses related to the 2016 epidemic as well as a reassortant strain might indicate undetected circulation of the virus in resident wild birds or in the poultry sector. To avoid the concealed circulation and evolution of viruses, and the risk of emergence of strains with pandemic potential, the implementation of control measures is of utmost importance, particularly in duck farms where birds display no clinical signs. MDPI 2021-10-16 /pmc/articles/PMC8541051/ /pubmed/34696516 http://dx.doi.org/10.3390/v13102086 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zecchin, Bianca
Goujgoulova, Gabriela
Monne, Isabella
Salviato, Annalisa
Schivo, Alessia
Slavcheva, Iskra
Pastori, Ambra
Brown, Ian H.
Lewis, Nicola S.
Terregino, Calogero
Fusaro, Alice
Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021
title Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021
title_full Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021
title_fullStr Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021
title_full_unstemmed Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021
title_short Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021
title_sort evolutionary dynamics of h5 highly pathogenic avian influenza viruses (clade 2.3.4.4b) circulating in bulgaria in 2019–2021
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541051/
https://www.ncbi.nlm.nih.gov/pubmed/34696516
http://dx.doi.org/10.3390/v13102086
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