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Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis
Small peptides and proteins play critical regulatory roles in plant development and environmental stress responses; however, only a few of these molecules have been identified and characterized to date because of their poor annotation and other experimental challenges. Here, we present that rice (Or...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541125/ https://www.ncbi.nlm.nih.gov/pubmed/34685986 http://dx.doi.org/10.3390/plants10102181 |
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author | Kim, Sung-Il Lee, Kyu Ho Kwak, Jun Soo Kwon, Dae Hwan Song, Jong Tae Seo, Hak Soo |
author_facet | Kim, Sung-Il Lee, Kyu Ho Kwak, Jun Soo Kwon, Dae Hwan Song, Jong Tae Seo, Hak Soo |
author_sort | Kim, Sung-Il |
collection | PubMed |
description | Small peptides and proteins play critical regulatory roles in plant development and environmental stress responses; however, only a few of these molecules have been identified and characterized to date because of their poor annotation and other experimental challenges. Here, we present that rice (Oryza sativa L.) OsS1Fa1, a small 76-amino acid protein, confers drought stress tolerance in Arabidopsis thaliana. OsS1Fa1 was highly expressed in leaf, culm, and root tissues of rice seedlings during vegetative growth and was significantly induced under drought stress. OsS1Fa1 overexpression in Arabidopsis induced the expression of selected drought-responsive genes and enhanced the survival rate of transgenic lines under drought. The proteasome inhibitor MG132 protected the OsS1Fa1 protein from degradation. Together, our data indicate that the small protein OsS1Fa1 is induced by drought and is post-translationally regulated, and the ectopic expression of OsS1Fa1 protects plants from drought stress. |
format | Online Article Text |
id | pubmed-8541125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85411252021-10-24 Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis Kim, Sung-Il Lee, Kyu Ho Kwak, Jun Soo Kwon, Dae Hwan Song, Jong Tae Seo, Hak Soo Plants (Basel) Article Small peptides and proteins play critical regulatory roles in plant development and environmental stress responses; however, only a few of these molecules have been identified and characterized to date because of their poor annotation and other experimental challenges. Here, we present that rice (Oryza sativa L.) OsS1Fa1, a small 76-amino acid protein, confers drought stress tolerance in Arabidopsis thaliana. OsS1Fa1 was highly expressed in leaf, culm, and root tissues of rice seedlings during vegetative growth and was significantly induced under drought stress. OsS1Fa1 overexpression in Arabidopsis induced the expression of selected drought-responsive genes and enhanced the survival rate of transgenic lines under drought. The proteasome inhibitor MG132 protected the OsS1Fa1 protein from degradation. Together, our data indicate that the small protein OsS1Fa1 is induced by drought and is post-translationally regulated, and the ectopic expression of OsS1Fa1 protects plants from drought stress. MDPI 2021-10-14 /pmc/articles/PMC8541125/ /pubmed/34685986 http://dx.doi.org/10.3390/plants10102181 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Sung-Il Lee, Kyu Ho Kwak, Jun Soo Kwon, Dae Hwan Song, Jong Tae Seo, Hak Soo Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis |
title | Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis |
title_full | Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis |
title_fullStr | Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis |
title_full_unstemmed | Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis |
title_short | Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis |
title_sort | overexpression of rice oss1fa1 gene confers drought tolerance in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541125/ https://www.ncbi.nlm.nih.gov/pubmed/34685986 http://dx.doi.org/10.3390/plants10102181 |
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