Cargando…

The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection

Bovine papillomavirus (BPV) types 1 and 2 are recognized as the main cause of equine sarcoids. However, some studies report that up to a quarter of these tumors do not contain detectible BPV1 or BPV2 DNA. The absence of detectible BPV1 or BPV2 in these sarcoids suggests the possible involvement of o...

Descripción completa

Detalles Bibliográficos
Autores principales: Munday, John S., Gedye, Kristene, Daudt, Cíntia, Chaves Da Silva, Flavio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541201/
https://www.ncbi.nlm.nih.gov/pubmed/34679038
http://dx.doi.org/10.3390/vetsci8100208
_version_ 1784589172370046976
author Munday, John S.
Gedye, Kristene
Daudt, Cíntia
Chaves Da Silva, Flavio
author_facet Munday, John S.
Gedye, Kristene
Daudt, Cíntia
Chaves Da Silva, Flavio
author_sort Munday, John S.
collection PubMed
description Bovine papillomavirus (BPV) types 1 and 2 are recognized as the main cause of equine sarcoids. However, some studies report that up to a quarter of these tumors do not contain detectible BPV1 or BPV2 DNA. The absence of detectible BPV1 or BPV2 in these sarcoids suggests the possible involvement of other papillomavirus types. Currently, five deltapapillomaviruses are recognized to cause mesenchymal neoplasia after cross-species infection. In addition to BPV1 and BPV2, BPV13 has been associated with equine sarcoids in Brazil, BPV14 has been associated with feline sarcoids, and Ovis aries papillomavirus 2 caused a sarcoid-like lesion in a pig. To investigate the cause of equine sarcoids, PCR primers were developed to specifically amplify each of the five different deltapapillomaviruses that have been associated with mesenchymal neoplasia. The specificity of these primers was confirmed using samples of formalin-fixed tissue known to contain each PV type. These primers allow rapid and sensitive detection of deltapapillomavirus DNA in equine sarcoids. As studies have revealed marked regional variability in the cause of equine sarcoids, these primers will be useful to determine the predominant PV type causing sarcoids in a region. Additionally, there is a single report describing mixed infections by BPV1 and BPV2 in equine sarcoids. The specific primer sets are expected to enable more sensitive detection of mixed infections in equine sarcoids. Determining the cause of equine sarcoids is important as vaccines are developed to prevent these common malignant neoplasms.
format Online
Article
Text
id pubmed-8541201
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85412012021-10-24 The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection Munday, John S. Gedye, Kristene Daudt, Cíntia Chaves Da Silva, Flavio Vet Sci Communication Bovine papillomavirus (BPV) types 1 and 2 are recognized as the main cause of equine sarcoids. However, some studies report that up to a quarter of these tumors do not contain detectible BPV1 or BPV2 DNA. The absence of detectible BPV1 or BPV2 in these sarcoids suggests the possible involvement of other papillomavirus types. Currently, five deltapapillomaviruses are recognized to cause mesenchymal neoplasia after cross-species infection. In addition to BPV1 and BPV2, BPV13 has been associated with equine sarcoids in Brazil, BPV14 has been associated with feline sarcoids, and Ovis aries papillomavirus 2 caused a sarcoid-like lesion in a pig. To investigate the cause of equine sarcoids, PCR primers were developed to specifically amplify each of the five different deltapapillomaviruses that have been associated with mesenchymal neoplasia. The specificity of these primers was confirmed using samples of formalin-fixed tissue known to contain each PV type. These primers allow rapid and sensitive detection of deltapapillomavirus DNA in equine sarcoids. As studies have revealed marked regional variability in the cause of equine sarcoids, these primers will be useful to determine the predominant PV type causing sarcoids in a region. Additionally, there is a single report describing mixed infections by BPV1 and BPV2 in equine sarcoids. The specific primer sets are expected to enable more sensitive detection of mixed infections in equine sarcoids. Determining the cause of equine sarcoids is important as vaccines are developed to prevent these common malignant neoplasms. MDPI 2021-09-26 /pmc/articles/PMC8541201/ /pubmed/34679038 http://dx.doi.org/10.3390/vetsci8100208 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Munday, John S.
Gedye, Kristene
Daudt, Cíntia
Chaves Da Silva, Flavio
The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection
title The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection
title_full The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection
title_fullStr The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection
title_full_unstemmed The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection
title_short The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection
title_sort development of novel primer sets to specifically amplify each of the five different deltapapillomaviruses that cause neoplasia after cross-species infection
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541201/
https://www.ncbi.nlm.nih.gov/pubmed/34679038
http://dx.doi.org/10.3390/vetsci8100208
work_keys_str_mv AT mundayjohns thedevelopmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection
AT gedyekristene thedevelopmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection
AT daudtcintia thedevelopmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection
AT chavesdasilvaflavio thedevelopmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection
AT mundayjohns developmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection
AT gedyekristene developmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection
AT daudtcintia developmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection
AT chavesdasilvaflavio developmentofnovelprimersetstospecificallyamplifyeachofthefivedifferentdeltapapillomavirusesthatcauseneoplasiaaftercrossspeciesinfection