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Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes

Arsenic is one of the most hazardous metalloids in nature, and due to its high water solubility, it is one of the most important causes of pollution. However, silicon reduces the uptake and transport of arsenic in rice. This study investigates the interaction of different arsenic and silicon levels...

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Autores principales: Boorboori, Mohammad Reza, Li, Zhou, Yan, Xue, Dan, Mu, Zhang, Zhixing, Lin, Wenxiong, Fang, Changxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541284/
https://www.ncbi.nlm.nih.gov/pubmed/34686019
http://dx.doi.org/10.3390/plants10102210
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author Boorboori, Mohammad Reza
Li, Zhou
Yan, Xue
Dan, Mu
Zhang, Zhixing
Lin, Wenxiong
Fang, Changxun
author_facet Boorboori, Mohammad Reza
Li, Zhou
Yan, Xue
Dan, Mu
Zhang, Zhixing
Lin, Wenxiong
Fang, Changxun
author_sort Boorboori, Mohammad Reza
collection PubMed
description Arsenic is one of the most hazardous metalloids in nature, and due to its high water solubility, it is one of the most important causes of pollution. However, silicon reduces the uptake and transport of arsenic in rice. This study investigates the interaction of different arsenic and silicon levels on dry weight, protein content, and concentrations of arsenic and silicon in two different rice shoots and roots of Dular wild-type (DU-WT) and Dular Lsi1-overexpressed (DU-OE) rice. It should be noted that all seedlings were subjected to four different treatments. For RNA-seq and qPCR, the DU-WT genotype was selected as the control and DU-OE as the treatment. With the addition of silicone treatment, dry weight and protein content in the shoots and roots of both rice lines were increased, while the concentration of arsenic in these two organs was decreased. When seedlings were exposed to arsenic treatments, protein content, silicon concentration, and dry weight were decreased in both roots and shoots, while arsenic concentration was increased in both rice genotypes. The RNA-seq in DU-OE showed 5823 differentially expressed genes (DEGs), of which 2604 were up-regulated and 3219 down-regulated. Treatment of rice by arsenic and silicon has changed the expression of genes encoding cytokinin-responsive GATA transcription factor 1, protein IN2-1 homolog B, calcium-binding EGF domain-containing protein, Os01g0369700 protein, probable glutathione S-transferase GSTU1, glutathione S-transferase protein, Os09g0367700 protein, isocitrate dehydrogenase (NADP), and Os08g0522400 protein in the root of DU-OE. The present study’s findings showed that in the presence of silicon, the transgenic genotype is much more resistant to arsenic than the wild genotype of Dular rice.
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spelling pubmed-85412842021-10-24 Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes Boorboori, Mohammad Reza Li, Zhou Yan, Xue Dan, Mu Zhang, Zhixing Lin, Wenxiong Fang, Changxun Plants (Basel) Article Arsenic is one of the most hazardous metalloids in nature, and due to its high water solubility, it is one of the most important causes of pollution. However, silicon reduces the uptake and transport of arsenic in rice. This study investigates the interaction of different arsenic and silicon levels on dry weight, protein content, and concentrations of arsenic and silicon in two different rice shoots and roots of Dular wild-type (DU-WT) and Dular Lsi1-overexpressed (DU-OE) rice. It should be noted that all seedlings were subjected to four different treatments. For RNA-seq and qPCR, the DU-WT genotype was selected as the control and DU-OE as the treatment. With the addition of silicone treatment, dry weight and protein content in the shoots and roots of both rice lines were increased, while the concentration of arsenic in these two organs was decreased. When seedlings were exposed to arsenic treatments, protein content, silicon concentration, and dry weight were decreased in both roots and shoots, while arsenic concentration was increased in both rice genotypes. The RNA-seq in DU-OE showed 5823 differentially expressed genes (DEGs), of which 2604 were up-regulated and 3219 down-regulated. Treatment of rice by arsenic and silicon has changed the expression of genes encoding cytokinin-responsive GATA transcription factor 1, protein IN2-1 homolog B, calcium-binding EGF domain-containing protein, Os01g0369700 protein, probable glutathione S-transferase GSTU1, glutathione S-transferase protein, Os09g0367700 protein, isocitrate dehydrogenase (NADP), and Os08g0522400 protein in the root of DU-OE. The present study’s findings showed that in the presence of silicon, the transgenic genotype is much more resistant to arsenic than the wild genotype of Dular rice. MDPI 2021-10-18 /pmc/articles/PMC8541284/ /pubmed/34686019 http://dx.doi.org/10.3390/plants10102210 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boorboori, Mohammad Reza
Li, Zhou
Yan, Xue
Dan, Mu
Zhang, Zhixing
Lin, Wenxiong
Fang, Changxun
Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes
title Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes
title_full Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes
title_fullStr Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes
title_full_unstemmed Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes
title_short Comparison of Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes
title_sort comparison of silicon-evoked responses on arsenic stress between different dular rice genotypes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541284/
https://www.ncbi.nlm.nih.gov/pubmed/34686019
http://dx.doi.org/10.3390/plants10102210
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