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Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones
Salvia miltiorrhiza (Labiatae) is an important medicinal plant in traditional Chinese medicine. Tanshinones are one of the main active components of S. miltiorrhiza. It has been found that the intraspecific variation of S. miltiorrhiza is relatively large and the content of tanshinones in its roots...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541307/ https://www.ncbi.nlm.nih.gov/pubmed/34721983 http://dx.doi.org/10.7717/peerj.12300 |
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author | Zhou, Jingwen Liu, Rui Shuai, Min Yan, Zhu-Yun Chen, Xin |
author_facet | Zhou, Jingwen Liu, Rui Shuai, Min Yan, Zhu-Yun Chen, Xin |
author_sort | Zhou, Jingwen |
collection | PubMed |
description | Salvia miltiorrhiza (Labiatae) is an important medicinal plant in traditional Chinese medicine. Tanshinones are one of the main active components of S. miltiorrhiza. It has been found that the intraspecific variation of S. miltiorrhiza is relatively large and the content of tanshinones in its roots of different varieties is also relatively different. To investigate the molecular mechanisms that responsible for the differences among these varieties, the tanshinones content was determined and comparative transcriptomics analysis was carried out during the tanshinones accumulation stage. A total of 52,216 unigenes were obtained from the transcriptome by RNA sequencing among which 23,369 genes were differentially expressed among different varieties, and 2,016 genes including 18 diterpenoid biosynthesis-related genes were differentially expressed during the tanshinones accumulation stage. Functional categorization of the differentially expressed genes (DEGs) among these varieties revealed that the pathway related to photosynthesis, oxidative phosphorylation, secondary metabolite biosynthesis, diterpenoid biosynthesis, terpenoid backbone biosynthesis, sesquiterpenoid and triterpenoid biosynthesis are the most differentially regulated processes in these varieties. The six tanshinone components in these varieties showed different dynamic changes in tanshinone accumulation stage. In addition, combined with the analysis of the dynamic changes, 277 DEGs (including one dehydrogenase, three CYP450 and 24 transcription factors belonging to 12 transcription factor families) related to the accumulation of tanshinones components were obtained. Furthermore, the KEGG pathway enrichment analysis of these 277 DEGs suggested that there might be an interconnection between the primary metabolic processes, signaling processes and the accumulation of tanshinones components. This study expands the vision of intraspecific variation and gene regulation mechanism of secondary metabolite biosynthesis pathways in medicinal plants from the “omics” perspective. |
format | Online Article Text |
id | pubmed-8541307 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85413072021-10-29 Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones Zhou, Jingwen Liu, Rui Shuai, Min Yan, Zhu-Yun Chen, Xin PeerJ Biodiversity Salvia miltiorrhiza (Labiatae) is an important medicinal plant in traditional Chinese medicine. Tanshinones are one of the main active components of S. miltiorrhiza. It has been found that the intraspecific variation of S. miltiorrhiza is relatively large and the content of tanshinones in its roots of different varieties is also relatively different. To investigate the molecular mechanisms that responsible for the differences among these varieties, the tanshinones content was determined and comparative transcriptomics analysis was carried out during the tanshinones accumulation stage. A total of 52,216 unigenes were obtained from the transcriptome by RNA sequencing among which 23,369 genes were differentially expressed among different varieties, and 2,016 genes including 18 diterpenoid biosynthesis-related genes were differentially expressed during the tanshinones accumulation stage. Functional categorization of the differentially expressed genes (DEGs) among these varieties revealed that the pathway related to photosynthesis, oxidative phosphorylation, secondary metabolite biosynthesis, diterpenoid biosynthesis, terpenoid backbone biosynthesis, sesquiterpenoid and triterpenoid biosynthesis are the most differentially regulated processes in these varieties. The six tanshinone components in these varieties showed different dynamic changes in tanshinone accumulation stage. In addition, combined with the analysis of the dynamic changes, 277 DEGs (including one dehydrogenase, three CYP450 and 24 transcription factors belonging to 12 transcription factor families) related to the accumulation of tanshinones components were obtained. Furthermore, the KEGG pathway enrichment analysis of these 277 DEGs suggested that there might be an interconnection between the primary metabolic processes, signaling processes and the accumulation of tanshinones components. This study expands the vision of intraspecific variation and gene regulation mechanism of secondary metabolite biosynthesis pathways in medicinal plants from the “omics” perspective. PeerJ Inc. 2021-10-20 /pmc/articles/PMC8541307/ /pubmed/34721983 http://dx.doi.org/10.7717/peerj.12300 Text en © 2021 Zhou et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biodiversity Zhou, Jingwen Liu, Rui Shuai, Min Yan, Zhu-Yun Chen, Xin Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones |
title | Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones |
title_full | Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones |
title_fullStr | Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones |
title_full_unstemmed | Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones |
title_short | Comparative transcriptome analyses of different Salvia miltiorrhiza varieties during the accumulation of tanshinones |
title_sort | comparative transcriptome analyses of different salvia miltiorrhiza varieties during the accumulation of tanshinones |
topic | Biodiversity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541307/ https://www.ncbi.nlm.nih.gov/pubmed/34721983 http://dx.doi.org/10.7717/peerj.12300 |
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