Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae

SIMPLE SUMMARY: Nosema ceranae affects honey bee (Apis mellifera L.) causing nosemosis disease that often induces serious problems in apiculture. Antibiotic fumagillin is the only licenced treatment against nosemosis, but its effectiveness is questioned and its usage associated with risk of bee mortality and appearance of residues in bee products. In search for alternative treatment for the control of nosemosis, water crude extract of Agaricus bisporus was tested on bees in laboratory (cage) experiments. Bee survival and food consumption were monitored together with Nosema infection level and expression of five genes (abaecin, hymenoptaecin, defensin, apidaecin, and vitellogenin) were evaluated in bees sampled on days 7 and 15. Apart from the gene for defensin, the expression of all tested genes was up-regulated in bees supplemented with A. bisporus extract. Both anti-Nosema and immune protective effects of A. bisporus extract were observed when supplementation started at the moment of N. ceranae infection or preventively (before or simultaneously with the Nosema infection). ABSTRACT: Agaricus bisporus water crude extract was tested on honey bees for the first time. The first part of the cage experiment was set for selecting one concentration of the A. bisporus extract. Concentration of 200 µg/g was further tested in the second part of the experiment where bee survival and food consumption were monitored together with Nosema infection level and expression of five genes (abaecin, hymenoptaecin, defensin, apidaecin, and vitellogenin) that were evaluated in bees sampled on days 7 and 15. Survival rate of Nosema-infected bees was significantly greater in groups fed with A. bisporus-enriched syrup compared to those fed with a pure sucrose syrup. Besides, the anti-Nosema effect of A. bisporus extract was greatest when applied from the third day which coincides with the time of infection with N. ceranae. Daily food consumption did not differ between the groups indicating good acceptability and palatability of the extract. A. bisporus extract showed a stimulative effect on four out of five monitored genes. Both anti-Nosema and nutrigenomic effects of A. bisporus extract were observed when supplementation started at the moment of N. ceranae infection or preventively (before or simultaneously with the infection).