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Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the bi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541337/ https://www.ncbi.nlm.nih.gov/pubmed/34684837 http://dx.doi.org/10.3390/molecules26206258 |
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author | Spreda, Miriam Hauptmann, Nicole Lehner, Veronika Biehl, Christoph Liefeith, Klaus Lips, Katrin Susanne |
author_facet | Spreda, Miriam Hauptmann, Nicole Lehner, Veronika Biehl, Christoph Liefeith, Klaus Lips, Katrin Susanne |
author_sort | Spreda, Miriam |
collection | PubMed |
description | In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity. |
format | Online Article Text |
id | pubmed-8541337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85413372021-10-24 Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity Spreda, Miriam Hauptmann, Nicole Lehner, Veronika Biehl, Christoph Liefeith, Klaus Lips, Katrin Susanne Molecules Article In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity. MDPI 2021-10-16 /pmc/articles/PMC8541337/ /pubmed/34684837 http://dx.doi.org/10.3390/molecules26206258 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Spreda, Miriam Hauptmann, Nicole Lehner, Veronika Biehl, Christoph Liefeith, Klaus Lips, Katrin Susanne Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
title | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
title_full | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
title_fullStr | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
title_full_unstemmed | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
title_short | Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity |
title_sort | porous 3d scaffolds enhance msc vitality and reduce osteoclast activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541337/ https://www.ncbi.nlm.nih.gov/pubmed/34684837 http://dx.doi.org/10.3390/molecules26206258 |
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