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Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity

In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the bi...

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Autores principales: Spreda, Miriam, Hauptmann, Nicole, Lehner, Veronika, Biehl, Christoph, Liefeith, Klaus, Lips, Katrin Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541337/
https://www.ncbi.nlm.nih.gov/pubmed/34684837
http://dx.doi.org/10.3390/molecules26206258
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author Spreda, Miriam
Hauptmann, Nicole
Lehner, Veronika
Biehl, Christoph
Liefeith, Klaus
Lips, Katrin Susanne
author_facet Spreda, Miriam
Hauptmann, Nicole
Lehner, Veronika
Biehl, Christoph
Liefeith, Klaus
Lips, Katrin Susanne
author_sort Spreda, Miriam
collection PubMed
description In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity.
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spelling pubmed-85413372021-10-24 Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity Spreda, Miriam Hauptmann, Nicole Lehner, Veronika Biehl, Christoph Liefeith, Klaus Lips, Katrin Susanne Molecules Article In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity. MDPI 2021-10-16 /pmc/articles/PMC8541337/ /pubmed/34684837 http://dx.doi.org/10.3390/molecules26206258 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spreda, Miriam
Hauptmann, Nicole
Lehner, Veronika
Biehl, Christoph
Liefeith, Klaus
Lips, Katrin Susanne
Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
title Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
title_full Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
title_fullStr Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
title_full_unstemmed Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
title_short Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity
title_sort porous 3d scaffolds enhance msc vitality and reduce osteoclast activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541337/
https://www.ncbi.nlm.nih.gov/pubmed/34684837
http://dx.doi.org/10.3390/molecules26206258
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