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The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer

Influenza A virus envelope contains lipid molecules of the host cell and three integral viral proteins: major hemagglutinin, neuraminidase, and minor M2 protein. Membrane-associated M1 matrix protein is thought to interact with the lipid bilayer and cytoplasmic domains of integral viral proteins to...

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Autores principales: Kordyukova, Larisa V., Konarev, Petr V., Fedorova, Nataliya V., Shtykova, Eleonora V., Ksenofontov, Alexander L., Loshkarev, Nikita A., Dadinova, Lubov A., Timofeeva, Tatyana A., Abramchuk, Sergei S., Moiseenko, Andrei V., Baratova, Lyudmila A., Svergun, Dmitri I., Batishchev, Oleg V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541430/
https://www.ncbi.nlm.nih.gov/pubmed/34677538
http://dx.doi.org/10.3390/membranes11100772
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author Kordyukova, Larisa V.
Konarev, Petr V.
Fedorova, Nataliya V.
Shtykova, Eleonora V.
Ksenofontov, Alexander L.
Loshkarev, Nikita A.
Dadinova, Lubov A.
Timofeeva, Tatyana A.
Abramchuk, Sergei S.
Moiseenko, Andrei V.
Baratova, Lyudmila A.
Svergun, Dmitri I.
Batishchev, Oleg V.
author_facet Kordyukova, Larisa V.
Konarev, Petr V.
Fedorova, Nataliya V.
Shtykova, Eleonora V.
Ksenofontov, Alexander L.
Loshkarev, Nikita A.
Dadinova, Lubov A.
Timofeeva, Tatyana A.
Abramchuk, Sergei S.
Moiseenko, Andrei V.
Baratova, Lyudmila A.
Svergun, Dmitri I.
Batishchev, Oleg V.
author_sort Kordyukova, Larisa V.
collection PubMed
description Influenza A virus envelope contains lipid molecules of the host cell and three integral viral proteins: major hemagglutinin, neuraminidase, and minor M2 protein. Membrane-associated M1 matrix protein is thought to interact with the lipid bilayer and cytoplasmic domains of integral viral proteins to form infectious virus progeny. We used small-angle X-ray scattering (SAXS) and complementary techniques to analyze the interactions of different components of the viral envelope with M1 matrix protein. Small unilamellar liposomes composed of various mixtures of synthetic or “native” lipids extracted from Influenza A/Puerto Rico/8/34 (H1N1) virions as well as proteoliposomes built from the viral lipids and anchored peptides of integral viral proteins (mainly, hemagglutinin) were incubated with isolated M1 and measured using SAXS. The results imply that M1 interaction with phosphatidylserine leads to condensation of the lipid in the protein-contacting monolayer, thus resulting in formation of lipid tubules. This effect vanishes in the presence of the liquid-ordered (raft-forming) constituents (sphingomyelin and cholesterol) regardless of their proportion in the lipid bilayer. We also detected a specific role of the hemagglutinin anchoring peptides in ordering of viral lipid membrane into the raft-like one. These peptides stimulate the oligomerization of M1 on the membrane to form a viral scaffold for subsequent budding of the virion from the plasma membrane of the infected cell.
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spelling pubmed-85414302021-10-24 The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer Kordyukova, Larisa V. Konarev, Petr V. Fedorova, Nataliya V. Shtykova, Eleonora V. Ksenofontov, Alexander L. Loshkarev, Nikita A. Dadinova, Lubov A. Timofeeva, Tatyana A. Abramchuk, Sergei S. Moiseenko, Andrei V. Baratova, Lyudmila A. Svergun, Dmitri I. Batishchev, Oleg V. Membranes (Basel) Article Influenza A virus envelope contains lipid molecules of the host cell and three integral viral proteins: major hemagglutinin, neuraminidase, and minor M2 protein. Membrane-associated M1 matrix protein is thought to interact with the lipid bilayer and cytoplasmic domains of integral viral proteins to form infectious virus progeny. We used small-angle X-ray scattering (SAXS) and complementary techniques to analyze the interactions of different components of the viral envelope with M1 matrix protein. Small unilamellar liposomes composed of various mixtures of synthetic or “native” lipids extracted from Influenza A/Puerto Rico/8/34 (H1N1) virions as well as proteoliposomes built from the viral lipids and anchored peptides of integral viral proteins (mainly, hemagglutinin) were incubated with isolated M1 and measured using SAXS. The results imply that M1 interaction with phosphatidylserine leads to condensation of the lipid in the protein-contacting monolayer, thus resulting in formation of lipid tubules. This effect vanishes in the presence of the liquid-ordered (raft-forming) constituents (sphingomyelin and cholesterol) regardless of their proportion in the lipid bilayer. We also detected a specific role of the hemagglutinin anchoring peptides in ordering of viral lipid membrane into the raft-like one. These peptides stimulate the oligomerization of M1 on the membrane to form a viral scaffold for subsequent budding of the virion from the plasma membrane of the infected cell. MDPI 2021-10-10 /pmc/articles/PMC8541430/ /pubmed/34677538 http://dx.doi.org/10.3390/membranes11100772 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kordyukova, Larisa V.
Konarev, Petr V.
Fedorova, Nataliya V.
Shtykova, Eleonora V.
Ksenofontov, Alexander L.
Loshkarev, Nikita A.
Dadinova, Lubov A.
Timofeeva, Tatyana A.
Abramchuk, Sergei S.
Moiseenko, Andrei V.
Baratova, Lyudmila A.
Svergun, Dmitri I.
Batishchev, Oleg V.
The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer
title The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer
title_full The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer
title_fullStr The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer
title_full_unstemmed The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer
title_short The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer
title_sort cytoplasmic tail of influenza a virus hemagglutinin and membrane lipid composition change the mode of m1 protein association with the lipid bilayer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541430/
https://www.ncbi.nlm.nih.gov/pubmed/34677538
http://dx.doi.org/10.3390/membranes11100772
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