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Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs
BACKGROUND: Dermatological infections are the most common cases in the daily pet clinic. Since its discovery in 1990, Staphylococcus schleiferi subspecies coagulans have been reported more frequently in canine otitis externa and pyoderma and even in cases of zoonoses. AIM: Detect the presence of S....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculty of Veterinary Medicine
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541725/ https://www.ncbi.nlm.nih.gov/pubmed/34722197 http://dx.doi.org/10.5455/OVJ.2021.v11.i3.5 |
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author | Palomino-Farfán, Joel André Vega, Luis Guillermo Alvarez Espinoza, Sonia Yenny Calle Magallanes, Sofia Gonzales Moreno, Juan José Siuce |
author_facet | Palomino-Farfán, Joel André Vega, Luis Guillermo Alvarez Espinoza, Sonia Yenny Calle Magallanes, Sofia Gonzales Moreno, Juan José Siuce |
author_sort | Palomino-Farfán, Joel André |
collection | PubMed |
description | BACKGROUND: Dermatological infections are the most common cases in the daily pet clinic. Since its discovery in 1990, Staphylococcus schleiferi subspecies coagulans have been reported more frequently in canine otitis externa and pyoderma and even in cases of zoonoses. AIM: Detect the presence of S. schleiferi subsp. coagulans of canine otitis externa and pyoderma, its antimicrobial resistance, and the presence of mecAgen. METHODS: Three-hundred-thirty-one swabs from dogs with otitis externa and pyoderma were cultured on bacteriological agar for bacterial isolation and subsequent biochemical and molecular identification. The identified S. schleiferi subsp. coagulans were evaluated for their antimicrobial susceptibility using the Kirby–Bauer technique, including an oxacillin disk, and subsequently, a PCR was run to identify which ones had the mecA gene. RESULTS: Thirty-four (22.97%) and twelve (6.56%) isolates were identified as S. schleiferi subspecies coagulans from otitis externa and pyoderma, respectively. Fluoroquinolones, the most widely used group of antibiotics in Peru, showed a susceptibility of 58.82% (20/34) in cases of otitis externa and 50% (6/12) in cases of canine pyoderma. Meanwhile, nitrofurantoin was the antibiotic with the best efficacy in both cases, with 97% (33/34) in otitis externa and 83% (10/12) in pyoderma. Furthermore, 40% (13/34) of S. schleiferi subsp. coagulans isolated from otitis externa were resistant to methicillin, and 85.29% (29/34) had the mecA gene. On the other hand, the only methicillin-resistant isolate from pyoderma was also the only one with a mecA gene. CONCLUSION: This study is the first report of S. schleiferi subsp. coagulans in Peru, finding a higher percentage than reported in other South American countries. |
format | Online Article Text |
id | pubmed-8541725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Faculty of Veterinary Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-85417252021-10-29 Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs Palomino-Farfán, Joel André Vega, Luis Guillermo Alvarez Espinoza, Sonia Yenny Calle Magallanes, Sofia Gonzales Moreno, Juan José Siuce Open Vet J Original Research BACKGROUND: Dermatological infections are the most common cases in the daily pet clinic. Since its discovery in 1990, Staphylococcus schleiferi subspecies coagulans have been reported more frequently in canine otitis externa and pyoderma and even in cases of zoonoses. AIM: Detect the presence of S. schleiferi subsp. coagulans of canine otitis externa and pyoderma, its antimicrobial resistance, and the presence of mecAgen. METHODS: Three-hundred-thirty-one swabs from dogs with otitis externa and pyoderma were cultured on bacteriological agar for bacterial isolation and subsequent biochemical and molecular identification. The identified S. schleiferi subsp. coagulans were evaluated for their antimicrobial susceptibility using the Kirby–Bauer technique, including an oxacillin disk, and subsequently, a PCR was run to identify which ones had the mecA gene. RESULTS: Thirty-four (22.97%) and twelve (6.56%) isolates were identified as S. schleiferi subspecies coagulans from otitis externa and pyoderma, respectively. Fluoroquinolones, the most widely used group of antibiotics in Peru, showed a susceptibility of 58.82% (20/34) in cases of otitis externa and 50% (6/12) in cases of canine pyoderma. Meanwhile, nitrofurantoin was the antibiotic with the best efficacy in both cases, with 97% (33/34) in otitis externa and 83% (10/12) in pyoderma. Furthermore, 40% (13/34) of S. schleiferi subsp. coagulans isolated from otitis externa were resistant to methicillin, and 85.29% (29/34) had the mecA gene. On the other hand, the only methicillin-resistant isolate from pyoderma was also the only one with a mecA gene. CONCLUSION: This study is the first report of S. schleiferi subsp. coagulans in Peru, finding a higher percentage than reported in other South American countries. Faculty of Veterinary Medicine 2021 2021-07-20 /pmc/articles/PMC8541725/ /pubmed/34722197 http://dx.doi.org/10.5455/OVJ.2021.v11.i3.5 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Palomino-Farfán, Joel André Vega, Luis Guillermo Alvarez Espinoza, Sonia Yenny Calle Magallanes, Sofia Gonzales Moreno, Juan José Siuce Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs |
title | Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs |
title_full | Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs |
title_fullStr | Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs |
title_full_unstemmed | Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs |
title_short | Methicillin-resistant Staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs |
title_sort | methicillin-resistant staphylococcus schleiferi subspecies coagulans associated with otitis externa and pyoderma in dogs |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541725/ https://www.ncbi.nlm.nih.gov/pubmed/34722197 http://dx.doi.org/10.5455/OVJ.2021.v11.i3.5 |
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