Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a

In this study, Lba Cas12a (Cpf1) as one of the CRISPR systems from Lachnospiraceae bacterium was coupled with a hybridization chain reaction (HCR) to develop an electrochemical biosensor for detecting the pathogenic bacterium, Salmonella typhimurium. Autonomous cross-opening of functional DNA hairpi...

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Detalles Bibliográficos
Autores principales: Liu, Xiu, Bu, Shengjun, Feng, Jingqi, Wei, Hongguo, Wang, Ze, Li, Xue, Zhou, Hongyu, He, Xiuxia, Wan, Jiayu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542504/
https://www.ncbi.nlm.nih.gov/pubmed/34693471
http://dx.doi.org/10.1007/s00216-021-03733-6

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