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Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet

An excessive high-fat/energy diet is a major cause of obesity and linked complications, such as non-alcoholic fatty liver disease (NAFLD). Betaine has been shown to effectively improve hepatic lipid metabolism. However, the mechanistic basis for this improvement is largely unknown. Herein, integrati...

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Autores principales: Huang, Tengda, Yu, Lin, Pan, Hongyuan, Ma, Zeqiang, Wu, Tian, Zhang, Lifang, Liu, Kang, Qi, Qi, Miao, Weiwei, Song, Ziyi, Zhang, Haojie, Zhou, Lei, Li, Yixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542779/
https://www.ncbi.nlm.nih.gov/pubmed/34708066
http://dx.doi.org/10.3389/fnut.2021.751436
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author Huang, Tengda
Yu, Lin
Pan, Hongyuan
Ma, Zeqiang
Wu, Tian
Zhang, Lifang
Liu, Kang
Qi, Qi
Miao, Weiwei
Song, Ziyi
Zhang, Haojie
Zhou, Lei
Li, Yixing
author_facet Huang, Tengda
Yu, Lin
Pan, Hongyuan
Ma, Zeqiang
Wu, Tian
Zhang, Lifang
Liu, Kang
Qi, Qi
Miao, Weiwei
Song, Ziyi
Zhang, Haojie
Zhou, Lei
Li, Yixing
author_sort Huang, Tengda
collection PubMed
description An excessive high-fat/energy diet is a major cause of obesity and linked complications, such as non-alcoholic fatty liver disease (NAFLD). Betaine has been shown to effectively improve hepatic lipid metabolism. However, the mechanistic basis for this improvement is largely unknown. Herein, integration of mRNA sequencing and ribosome footprints profiling (Ribo-seq) was used to investigate the means by which betaine alleviates liver lipid metabolic disorders induced by a high-fat diet. For the transcriptome, gene set enrichment analysis demonstrated betaine to reduce liver steatosis by up-regulation of fatty acid beta oxidation, lipid oxidation, and fatty acid catabolic processes. For the translatome, 574 differentially expressed genes were identified, 17 of which were associated with the NAFLD pathway. By combined analysis of transcriptome and translatome, we found that betaine had the greater effect on NAFLD at the translational level. Further, betaine decreased translational efficiency (TE) for IDI1, CYP51A1, TM7SF2, and APOA4, which are related to lipid biosynthesis. In summary, this study demonstrated betaine alleviating lipid metabolic dysfunction at the translational level. The transcriptome and translatome data integration approach used herein provides for a new understanding of the means by which to treat NAFLD.
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spelling pubmed-85427792021-10-26 Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet Huang, Tengda Yu, Lin Pan, Hongyuan Ma, Zeqiang Wu, Tian Zhang, Lifang Liu, Kang Qi, Qi Miao, Weiwei Song, Ziyi Zhang, Haojie Zhou, Lei Li, Yixing Front Nutr Nutrition An excessive high-fat/energy diet is a major cause of obesity and linked complications, such as non-alcoholic fatty liver disease (NAFLD). Betaine has been shown to effectively improve hepatic lipid metabolism. However, the mechanistic basis for this improvement is largely unknown. Herein, integration of mRNA sequencing and ribosome footprints profiling (Ribo-seq) was used to investigate the means by which betaine alleviates liver lipid metabolic disorders induced by a high-fat diet. For the transcriptome, gene set enrichment analysis demonstrated betaine to reduce liver steatosis by up-regulation of fatty acid beta oxidation, lipid oxidation, and fatty acid catabolic processes. For the translatome, 574 differentially expressed genes were identified, 17 of which were associated with the NAFLD pathway. By combined analysis of transcriptome and translatome, we found that betaine had the greater effect on NAFLD at the translational level. Further, betaine decreased translational efficiency (TE) for IDI1, CYP51A1, TM7SF2, and APOA4, which are related to lipid biosynthesis. In summary, this study demonstrated betaine alleviating lipid metabolic dysfunction at the translational level. The transcriptome and translatome data integration approach used herein provides for a new understanding of the means by which to treat NAFLD. Frontiers Media S.A. 2021-10-11 /pmc/articles/PMC8542779/ /pubmed/34708066 http://dx.doi.org/10.3389/fnut.2021.751436 Text en Copyright © 2021 Huang, Yu, Pan, Ma, Wu, Zhang, Liu, Qi, Miao, Song, Zhang, Zhou and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Huang, Tengda
Yu, Lin
Pan, Hongyuan
Ma, Zeqiang
Wu, Tian
Zhang, Lifang
Liu, Kang
Qi, Qi
Miao, Weiwei
Song, Ziyi
Zhang, Haojie
Zhou, Lei
Li, Yixing
Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet
title Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet
title_full Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet
title_fullStr Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet
title_full_unstemmed Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet
title_short Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet
title_sort integrated transcriptomic and translatomic inquiry of the role of betaine on lipid metabolic dysregulation induced by a high-fat diet
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542779/
https://www.ncbi.nlm.nih.gov/pubmed/34708066
http://dx.doi.org/10.3389/fnut.2021.751436
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