Cargando…
A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2
Spermatocyte meiosis is the cornerstone of mammalian production. Thousands of long noncoding RNAs (lncRNAs) have been reported to be functional in various cellular processes, but the function of lncRNAs in meiosis remains largely unknown. Here, we profiled lncRNAs in spermatocytes at stage I of meio...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542969/ https://www.ncbi.nlm.nih.gov/pubmed/34707642 http://dx.doi.org/10.3389/fgene.2021.752495 |
_version_ | 1784589540879499264 |
---|---|
author | Liu, Wensheng Zhao, Yinan Liu, Xiaohua Zhang, Xiaoya Ding, Jiancheng Li, Yang Tian, Yingpu Wang, Haibin Liu, Wen Lu, Zhongxian |
author_facet | Liu, Wensheng Zhao, Yinan Liu, Xiaohua Zhang, Xiaoya Ding, Jiancheng Li, Yang Tian, Yingpu Wang, Haibin Liu, Wen Lu, Zhongxian |
author_sort | Liu, Wensheng |
collection | PubMed |
description | Spermatocyte meiosis is the cornerstone of mammalian production. Thousands of long noncoding RNAs (lncRNAs) have been reported to be functional in various cellular processes, but the function of lncRNAs in meiosis remains largely unknown. Here, we profiled lncRNAs in spermatocytes at stage I of meiosis and identified a testis-specific lncRNA, Rbakdn, as a vital regulator of meiosis. Rbakdn is dynamically expressed during meiosis I, and Rbakdn knockdown inhibits meiosis in vitro. Furthermore, Rbakdn knockdown in testes in mice by intratesticular injection disturbs meiosis, reduces testicular volume, and increases apoptosis of spermatocytes, resulting in vacuolation of the seminiferous tubules. Rbakdn can bind to Ptbp2, an RNA-binding protein that is important in the regulation of the alternative splicing of many genes in spermatogenesis. Rbakdn knockdown leads to a decrease in Ptbp2 through the ubiquitination degradation pathway, indicating that Rbakdn maintains the stability of Ptbp2. In conclusion, our study identified an lncRNA, Rbakdn, with a crucial role in meiosis. |
format | Online Article Text |
id | pubmed-8542969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85429692021-10-26 A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 Liu, Wensheng Zhao, Yinan Liu, Xiaohua Zhang, Xiaoya Ding, Jiancheng Li, Yang Tian, Yingpu Wang, Haibin Liu, Wen Lu, Zhongxian Front Genet Genetics Spermatocyte meiosis is the cornerstone of mammalian production. Thousands of long noncoding RNAs (lncRNAs) have been reported to be functional in various cellular processes, but the function of lncRNAs in meiosis remains largely unknown. Here, we profiled lncRNAs in spermatocytes at stage I of meiosis and identified a testis-specific lncRNA, Rbakdn, as a vital regulator of meiosis. Rbakdn is dynamically expressed during meiosis I, and Rbakdn knockdown inhibits meiosis in vitro. Furthermore, Rbakdn knockdown in testes in mice by intratesticular injection disturbs meiosis, reduces testicular volume, and increases apoptosis of spermatocytes, resulting in vacuolation of the seminiferous tubules. Rbakdn can bind to Ptbp2, an RNA-binding protein that is important in the regulation of the alternative splicing of many genes in spermatogenesis. Rbakdn knockdown leads to a decrease in Ptbp2 through the ubiquitination degradation pathway, indicating that Rbakdn maintains the stability of Ptbp2. In conclusion, our study identified an lncRNA, Rbakdn, with a crucial role in meiosis. Frontiers Media S.A. 2021-10-11 /pmc/articles/PMC8542969/ /pubmed/34707642 http://dx.doi.org/10.3389/fgene.2021.752495 Text en Copyright © 2021 Liu, Zhao, Liu, Zhang, Ding, Li, Tian, Wang, Liu and Lu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Liu, Wensheng Zhao, Yinan Liu, Xiaohua Zhang, Xiaoya Ding, Jiancheng Li, Yang Tian, Yingpu Wang, Haibin Liu, Wen Lu, Zhongxian A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 |
title | A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 |
title_full | A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 |
title_fullStr | A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 |
title_full_unstemmed | A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 |
title_short | A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 |
title_sort | novel meiosis-related lncrna, rbakdn, contributes to spermatogenesis by stabilizing ptbp2 |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542969/ https://www.ncbi.nlm.nih.gov/pubmed/34707642 http://dx.doi.org/10.3389/fgene.2021.752495 |
work_keys_str_mv | AT liuwensheng anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT zhaoyinan anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT liuxiaohua anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT zhangxiaoya anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT dingjiancheng anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT liyang anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT tianyingpu anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT wanghaibin anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT liuwen anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT luzhongxian anovelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT liuwensheng novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT zhaoyinan novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT liuxiaohua novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT zhangxiaoya novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT dingjiancheng novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT liyang novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT tianyingpu novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT wanghaibin novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT liuwen novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 AT luzhongxian novelmeiosisrelatedlncrnarbakdncontributestospermatogenesisbystabilizingptbp2 |