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A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2

Spermatocyte meiosis is the cornerstone of mammalian production. Thousands of long noncoding RNAs (lncRNAs) have been reported to be functional in various cellular processes, but the function of lncRNAs in meiosis remains largely unknown. Here, we profiled lncRNAs in spermatocytes at stage I of meio...

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Autores principales: Liu, Wensheng, Zhao, Yinan, Liu, Xiaohua, Zhang, Xiaoya, Ding, Jiancheng, Li, Yang, Tian, Yingpu, Wang, Haibin, Liu, Wen, Lu, Zhongxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542969/
https://www.ncbi.nlm.nih.gov/pubmed/34707642
http://dx.doi.org/10.3389/fgene.2021.752495
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author Liu, Wensheng
Zhao, Yinan
Liu, Xiaohua
Zhang, Xiaoya
Ding, Jiancheng
Li, Yang
Tian, Yingpu
Wang, Haibin
Liu, Wen
Lu, Zhongxian
author_facet Liu, Wensheng
Zhao, Yinan
Liu, Xiaohua
Zhang, Xiaoya
Ding, Jiancheng
Li, Yang
Tian, Yingpu
Wang, Haibin
Liu, Wen
Lu, Zhongxian
author_sort Liu, Wensheng
collection PubMed
description Spermatocyte meiosis is the cornerstone of mammalian production. Thousands of long noncoding RNAs (lncRNAs) have been reported to be functional in various cellular processes, but the function of lncRNAs in meiosis remains largely unknown. Here, we profiled lncRNAs in spermatocytes at stage I of meiosis and identified a testis-specific lncRNA, Rbakdn, as a vital regulator of meiosis. Rbakdn is dynamically expressed during meiosis I, and Rbakdn knockdown inhibits meiosis in vitro. Furthermore, Rbakdn knockdown in testes in mice by intratesticular injection disturbs meiosis, reduces testicular volume, and increases apoptosis of spermatocytes, resulting in vacuolation of the seminiferous tubules. Rbakdn can bind to Ptbp2, an RNA-binding protein that is important in the regulation of the alternative splicing of many genes in spermatogenesis. Rbakdn knockdown leads to a decrease in Ptbp2 through the ubiquitination degradation pathway, indicating that Rbakdn maintains the stability of Ptbp2. In conclusion, our study identified an lncRNA, Rbakdn, with a crucial role in meiosis.
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spelling pubmed-85429692021-10-26 A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2 Liu, Wensheng Zhao, Yinan Liu, Xiaohua Zhang, Xiaoya Ding, Jiancheng Li, Yang Tian, Yingpu Wang, Haibin Liu, Wen Lu, Zhongxian Front Genet Genetics Spermatocyte meiosis is the cornerstone of mammalian production. Thousands of long noncoding RNAs (lncRNAs) have been reported to be functional in various cellular processes, but the function of lncRNAs in meiosis remains largely unknown. Here, we profiled lncRNAs in spermatocytes at stage I of meiosis and identified a testis-specific lncRNA, Rbakdn, as a vital regulator of meiosis. Rbakdn is dynamically expressed during meiosis I, and Rbakdn knockdown inhibits meiosis in vitro. Furthermore, Rbakdn knockdown in testes in mice by intratesticular injection disturbs meiosis, reduces testicular volume, and increases apoptosis of spermatocytes, resulting in vacuolation of the seminiferous tubules. Rbakdn can bind to Ptbp2, an RNA-binding protein that is important in the regulation of the alternative splicing of many genes in spermatogenesis. Rbakdn knockdown leads to a decrease in Ptbp2 through the ubiquitination degradation pathway, indicating that Rbakdn maintains the stability of Ptbp2. In conclusion, our study identified an lncRNA, Rbakdn, with a crucial role in meiosis. Frontiers Media S.A. 2021-10-11 /pmc/articles/PMC8542969/ /pubmed/34707642 http://dx.doi.org/10.3389/fgene.2021.752495 Text en Copyright © 2021 Liu, Zhao, Liu, Zhang, Ding, Li, Tian, Wang, Liu and Lu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Liu, Wensheng
Zhao, Yinan
Liu, Xiaohua
Zhang, Xiaoya
Ding, Jiancheng
Li, Yang
Tian, Yingpu
Wang, Haibin
Liu, Wen
Lu, Zhongxian
A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2
title A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2
title_full A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2
title_fullStr A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2
title_full_unstemmed A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2
title_short A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2
title_sort novel meiosis-related lncrna, rbakdn, contributes to spermatogenesis by stabilizing ptbp2
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542969/
https://www.ncbi.nlm.nih.gov/pubmed/34707642
http://dx.doi.org/10.3389/fgene.2021.752495
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