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Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota
Evidence has demonstrated that the gut microbiota, which consists of probiotics and pathogenic microorganisms, is involved in the initiation of ulcerative colitis (UC) via the dysregulation of intestinal microflora and normal immune interactions, which ultimately leads to intestinal mucosal dysfunct...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8543469/ https://www.ncbi.nlm.nih.gov/pubmed/34707714 http://dx.doi.org/10.3892/etm.2021.10868 |
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author | Huangfu, Lu Xin Cai, Xin Tong Yang, Jing Nan Wang, Hui Chao Li, Yu Xia Dai, Zhi Feng Yang, Rui Lin Lin, Xu Hong |
author_facet | Huangfu, Lu Xin Cai, Xin Tong Yang, Jing Nan Wang, Hui Chao Li, Yu Xia Dai, Zhi Feng Yang, Rui Lin Lin, Xu Hong |
author_sort | Huangfu, Lu Xin |
collection | PubMed |
description | Evidence has demonstrated that the gut microbiota, which consists of probiotics and pathogenic microorganisms, is involved in the initiation of ulcerative colitis (UC) via the dysregulation of intestinal microflora and normal immune interactions, which ultimately leads to intestinal mucosal dysfunction. Irisin is released from muscle cells and displays anti-inflammatory effects; however, the mechanisms underlying irisin-mediated anti-inflammatory effects in UC have not been previously reported. In the present study, mice were divided into the following four groups: i) Control; ii) irisin; iii) dextran sulfate sodium (DSS) salt; and iv) DSS + irisin. Subsequently, the effects of irisin were investigated by observing alterations in intestinal microbes. Irisin significantly reduced the degree of inflammation in UC by reversing alterations to the macroscopic score, histological score, number of CD64(+) cells and inflammatory cytokine alterations (P<0.05). Analysis of the microbial diversity in the stools of mice with active UC indicated that the five bacteria that displayed the greatest alterations in relative abundance were Alloprevotella, Bacteroides, Lachnospiraceae-UCG-001, Prebotellaceae-UCG-001 and Rikenellaceae-RCB-gut-group. Furthermore, Bactoroides were positively correlated with the histopathological score (P=0.001; R=0.977) and interleukin (IL)-23 levels (P=0.008; R=0.924). Alloprevotella (P=0.001; R=-0.943), Lachnospiraceae-UCG-001 (P=0.000; R=-0.973) and Rikenollaceae-RC8-gut-group (P=0.001; R=-0.971) were negatively correlated with the histopathological score. Furthermore, Lachnospiraceae-UCG-001 (P=0.01; R=-0.873) and Rikenollaceae-RC8-gut-group (P=0.049; R=-0.814) were negatively correlated with IL-23 levels. In summary, the results of the present study suggested that irisin improved inflammation in a UC mouse model potentially via altering the gut microbiota. |
format | Online Article Text |
id | pubmed-8543469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-85434692021-10-26 Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota Huangfu, Lu Xin Cai, Xin Tong Yang, Jing Nan Wang, Hui Chao Li, Yu Xia Dai, Zhi Feng Yang, Rui Lin Lin, Xu Hong Exp Ther Med Articles Evidence has demonstrated that the gut microbiota, which consists of probiotics and pathogenic microorganisms, is involved in the initiation of ulcerative colitis (UC) via the dysregulation of intestinal microflora and normal immune interactions, which ultimately leads to intestinal mucosal dysfunction. Irisin is released from muscle cells and displays anti-inflammatory effects; however, the mechanisms underlying irisin-mediated anti-inflammatory effects in UC have not been previously reported. In the present study, mice were divided into the following four groups: i) Control; ii) irisin; iii) dextran sulfate sodium (DSS) salt; and iv) DSS + irisin. Subsequently, the effects of irisin were investigated by observing alterations in intestinal microbes. Irisin significantly reduced the degree of inflammation in UC by reversing alterations to the macroscopic score, histological score, number of CD64(+) cells and inflammatory cytokine alterations (P<0.05). Analysis of the microbial diversity in the stools of mice with active UC indicated that the five bacteria that displayed the greatest alterations in relative abundance were Alloprevotella, Bacteroides, Lachnospiraceae-UCG-001, Prebotellaceae-UCG-001 and Rikenellaceae-RCB-gut-group. Furthermore, Bactoroides were positively correlated with the histopathological score (P=0.001; R=0.977) and interleukin (IL)-23 levels (P=0.008; R=0.924). Alloprevotella (P=0.001; R=-0.943), Lachnospiraceae-UCG-001 (P=0.000; R=-0.973) and Rikenollaceae-RC8-gut-group (P=0.001; R=-0.971) were negatively correlated with the histopathological score. Furthermore, Lachnospiraceae-UCG-001 (P=0.01; R=-0.873) and Rikenollaceae-RC8-gut-group (P=0.049; R=-0.814) were negatively correlated with IL-23 levels. In summary, the results of the present study suggested that irisin improved inflammation in a UC mouse model potentially via altering the gut microbiota. D.A. Spandidos 2021-12 2021-10-11 /pmc/articles/PMC8543469/ /pubmed/34707714 http://dx.doi.org/10.3892/etm.2021.10868 Text en Copyright: © Huangfu et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Huangfu, Lu Xin Cai, Xin Tong Yang, Jing Nan Wang, Hui Chao Li, Yu Xia Dai, Zhi Feng Yang, Rui Lin Lin, Xu Hong Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota |
title | Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota |
title_full | Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota |
title_fullStr | Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota |
title_full_unstemmed | Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota |
title_short | Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota |
title_sort | irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8543469/ https://www.ncbi.nlm.nih.gov/pubmed/34707714 http://dx.doi.org/10.3892/etm.2021.10868 |
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