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Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)

ABSTRACT: BACKGROUND: Quinoa (Chenopodium quinoa) is a high-value grain known for its excellent nutritional balance. It is an allotetraploid species (AABB, 2n = 4x = 36) formed by the hybridization between AA and BB genome diploid (2n = 2x = 18) species. This study reports genetic studies in Chenopo...

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Autores principales: Subedi, Madhav, Neff, Erin, Davis, Thomas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8543794/
https://www.ncbi.nlm.nih.gov/pubmed/34696717
http://dx.doi.org/10.1186/s12870-021-03270-5
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author Subedi, Madhav
Neff, Erin
Davis, Thomas M.
author_facet Subedi, Madhav
Neff, Erin
Davis, Thomas M.
author_sort Subedi, Madhav
collection PubMed
description ABSTRACT: BACKGROUND: Quinoa (Chenopodium quinoa) is a high-value grain known for its excellent nutritional balance. It is an allotetraploid species (AABB, 2n = 4x = 36) formed by the hybridization between AA and BB genome diploid (2n = 2x = 18) species. This study reports genetic studies in Chenopodium ficifolium as a potential B genome diploid model system to simplify the genetic studies of quinoa including gene identification and marker-assisted breeding. RESULTS: Portsmouth, New Hampshire and Quebec City, Quebec accessions of C. ficifolium were used to develop an F2 population segregating for agronomically relevant traits including flowering time, plant height, the number of branches, branch angle, and internode length. Marker-trait associations were identified for the FLOWERING LOCUS T-LIKE 1 (FTL1) marker gene, where the alternate alleles (A1/A2) were segregating among the F2 generation plants in association with flowering time, plant height, and the number of branches. There was a strong correlation of the flowering time trait with both plant height and the number of branches. Thus, a possible multifaceted functional role for FTL1 may be considered. The parental Portsmouth and Quebec City accessions were homozygous for the alternate FTL1 alleles, which were found to be substantially diverged. SNPs were identified in the FTL1 coding sequence that could have some functional significance in relation to the observed trait variation. CONCLUSION: These results draw further attention to the possible functional roles of the FTL1 locus in Chenopodium and justify continued exploration of C. ficifolium as a potential diploid model system for the genetic study of quinoa. We expect our findings to aid in quinoa breeding as well as to any studies related to the Chenopodium genus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03270-5.
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spelling pubmed-85437942021-10-25 Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa) Subedi, Madhav Neff, Erin Davis, Thomas M. BMC Plant Biol Research ABSTRACT: BACKGROUND: Quinoa (Chenopodium quinoa) is a high-value grain known for its excellent nutritional balance. It is an allotetraploid species (AABB, 2n = 4x = 36) formed by the hybridization between AA and BB genome diploid (2n = 2x = 18) species. This study reports genetic studies in Chenopodium ficifolium as a potential B genome diploid model system to simplify the genetic studies of quinoa including gene identification and marker-assisted breeding. RESULTS: Portsmouth, New Hampshire and Quebec City, Quebec accessions of C. ficifolium were used to develop an F2 population segregating for agronomically relevant traits including flowering time, plant height, the number of branches, branch angle, and internode length. Marker-trait associations were identified for the FLOWERING LOCUS T-LIKE 1 (FTL1) marker gene, where the alternate alleles (A1/A2) were segregating among the F2 generation plants in association with flowering time, plant height, and the number of branches. There was a strong correlation of the flowering time trait with both plant height and the number of branches. Thus, a possible multifaceted functional role for FTL1 may be considered. The parental Portsmouth and Quebec City accessions were homozygous for the alternate FTL1 alleles, which were found to be substantially diverged. SNPs were identified in the FTL1 coding sequence that could have some functional significance in relation to the observed trait variation. CONCLUSION: These results draw further attention to the possible functional roles of the FTL1 locus in Chenopodium and justify continued exploration of C. ficifolium as a potential diploid model system for the genetic study of quinoa. We expect our findings to aid in quinoa breeding as well as to any studies related to the Chenopodium genus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03270-5. BioMed Central 2021-10-25 /pmc/articles/PMC8543794/ /pubmed/34696717 http://dx.doi.org/10.1186/s12870-021-03270-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Subedi, Madhav
Neff, Erin
Davis, Thomas M.
Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)
title Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)
title_full Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)
title_fullStr Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)
title_full_unstemmed Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)
title_short Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)
title_sort developing chenopodium ficifolium as a potential b genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (chenopodium quinoa)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8543794/
https://www.ncbi.nlm.nih.gov/pubmed/34696717
http://dx.doi.org/10.1186/s12870-021-03270-5
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