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Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease

BACKGROUND: Dysbiosis of the gut microbiome is involved in the pathogenesis of various diseases, but the contribution of gut microbes to the progression of chronic obstructive pulmonary disease (COPD) is still poorly understood. METHODS: We carried out 16S rRNA gene sequencing and short-chain fatty...

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Autores principales: Li, Naijian, Dai, Zhouli, Wang, Zhang, Deng, Zhishan, Zhang, Jiahuan, Pu, Jinding, Cao, Weitao, Pan, Tianhui, Zhou, Yumin, Yang, Zhaowei, Li, Jing, Li, Bing, Ran, Pixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8543848/
https://www.ncbi.nlm.nih.gov/pubmed/34696775
http://dx.doi.org/10.1186/s12931-021-01872-z
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author Li, Naijian
Dai, Zhouli
Wang, Zhang
Deng, Zhishan
Zhang, Jiahuan
Pu, Jinding
Cao, Weitao
Pan, Tianhui
Zhou, Yumin
Yang, Zhaowei
Li, Jing
Li, Bing
Ran, Pixin
author_facet Li, Naijian
Dai, Zhouli
Wang, Zhang
Deng, Zhishan
Zhang, Jiahuan
Pu, Jinding
Cao, Weitao
Pan, Tianhui
Zhou, Yumin
Yang, Zhaowei
Li, Jing
Li, Bing
Ran, Pixin
author_sort Li, Naijian
collection PubMed
description BACKGROUND: Dysbiosis of the gut microbiome is involved in the pathogenesis of various diseases, but the contribution of gut microbes to the progression of chronic obstructive pulmonary disease (COPD) is still poorly understood. METHODS: We carried out 16S rRNA gene sequencing and short-chain fatty acid analyses in stool samples from a cohort of 73 healthy controls, 67 patients with COPD of GOLD stages I and II severity, and 32 patients with COPD of GOLD stages III and IV severity. Fecal microbiota from the three groups were then inoculated into recipient mice for a total of 14 times in 28 days to induce pulmonary changes. Furthermore, fecal microbiota from the three groups were inoculated into mice exposed to smoke from biomass fuel to induce COPD-like changes. RESULTS: We observed that the gut microbiome of COPD patients varied from that of healthy controls and was characterized by a distinct overall microbial diversity and composition, a Prevotella-dominated gut enterotype and lower levels of short-chain fatty acids. After 28 days of fecal transplantation from COPD patients, recipient mice exhibited elevated lung inflammation. Moreover, when mice were under both fecal transplantation and biomass fuel smoke exposure for a total of 20 weeks, accelerated declines in lung function, severe emphysematous changes, airway remodeling and mucus hypersecretion were observed. CONCLUSION: These data demonstrate that altered gut microbiota in COPD patients is associated with disease progression in mice model. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-021-01872-z.
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spelling pubmed-85438482021-10-25 Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease Li, Naijian Dai, Zhouli Wang, Zhang Deng, Zhishan Zhang, Jiahuan Pu, Jinding Cao, Weitao Pan, Tianhui Zhou, Yumin Yang, Zhaowei Li, Jing Li, Bing Ran, Pixin Respir Res Research BACKGROUND: Dysbiosis of the gut microbiome is involved in the pathogenesis of various diseases, but the contribution of gut microbes to the progression of chronic obstructive pulmonary disease (COPD) is still poorly understood. METHODS: We carried out 16S rRNA gene sequencing and short-chain fatty acid analyses in stool samples from a cohort of 73 healthy controls, 67 patients with COPD of GOLD stages I and II severity, and 32 patients with COPD of GOLD stages III and IV severity. Fecal microbiota from the three groups were then inoculated into recipient mice for a total of 14 times in 28 days to induce pulmonary changes. Furthermore, fecal microbiota from the three groups were inoculated into mice exposed to smoke from biomass fuel to induce COPD-like changes. RESULTS: We observed that the gut microbiome of COPD patients varied from that of healthy controls and was characterized by a distinct overall microbial diversity and composition, a Prevotella-dominated gut enterotype and lower levels of short-chain fatty acids. After 28 days of fecal transplantation from COPD patients, recipient mice exhibited elevated lung inflammation. Moreover, when mice were under both fecal transplantation and biomass fuel smoke exposure for a total of 20 weeks, accelerated declines in lung function, severe emphysematous changes, airway remodeling and mucus hypersecretion were observed. CONCLUSION: These data demonstrate that altered gut microbiota in COPD patients is associated with disease progression in mice model. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-021-01872-z. BioMed Central 2021-10-25 2021 /pmc/articles/PMC8543848/ /pubmed/34696775 http://dx.doi.org/10.1186/s12931-021-01872-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Naijian
Dai, Zhouli
Wang, Zhang
Deng, Zhishan
Zhang, Jiahuan
Pu, Jinding
Cao, Weitao
Pan, Tianhui
Zhou, Yumin
Yang, Zhaowei
Li, Jing
Li, Bing
Ran, Pixin
Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease
title Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease
title_full Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease
title_fullStr Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease
title_full_unstemmed Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease
title_short Gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease
title_sort gut microbiota dysbiosis contributes to the development of chronic obstructive pulmonary disease
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8543848/
https://www.ncbi.nlm.nih.gov/pubmed/34696775
http://dx.doi.org/10.1186/s12931-021-01872-z
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