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Improved SARS-CoV-2 PCR detection and genotyping with double-bubble primers
A new approach for improved RT-PCR is described. It is based on primers designed to form controlled stem–loop and homodimer configurations, hence the name ‘double-bubble’ primers. The primers contain three main regions for efficient RT-PCR: a 3′ short overhang to allow reverse transcription, a stem...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Future Science Ltd
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544493/ https://www.ncbi.nlm.nih.gov/pubmed/34519222 http://dx.doi.org/10.2144/btn-2021-0063 |