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Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing

Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies investigating microbiomes. However, comparative studies on differences in procedures are scarce. We sequenced human stool samples and mock communities with increasing complexity using a variety of commonly used pro...

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Detalles Bibliográficos
Autores principales: Abellan-Schneyder, Isabel, Matchado, Monica S., Reitmeier, Sandra, Sommer, Alina, Sewald, Zeno, Baumbach, Jan, List, Markus, Neuhaus, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544895/
https://www.ncbi.nlm.nih.gov/pubmed/33627512
http://dx.doi.org/10.1128/mSphere.01202-20