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Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing
Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies investigating microbiomes. However, comparative studies on differences in procedures are scarce. We sequenced human stool samples and mock communities with increasing complexity using a variety of commonly used pro...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544895/ https://www.ncbi.nlm.nih.gov/pubmed/33627512 http://dx.doi.org/10.1128/mSphere.01202-20 |