The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity

To overcome CRISPR-Cas defense systems, many phages and mobile genetic elements (MGEs) encode CRISPR-Cas inhibitors called anti-CRISPRs (Acrs). Nearly all characterized Acrs directly bind Cas proteins to inactivate CRISPR immunity. Here, using functional metagenomic selection, we describe AcrIIA22,...

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Detalles Bibliográficos
Autores principales: Forsberg, Kevin J., Schmidtke, Danica T., Werther, Rachel, Uribe, Ruben V., Hausman, Deanna, Sommer, Morten O. A., Stoddard, Barry L., Kaiser, Brett K., Malik, Harmit S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545432/
https://www.ncbi.nlm.nih.gov/pubmed/34644300
http://dx.doi.org/10.1371/journal.pbio.3001428

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545432/
https://www.ncbi.nlm.nih.gov/pubmed/34644300
http://dx.doi.org/10.1371/journal.pbio.3001428

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