Cargando…

The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity

To overcome CRISPR-Cas defense systems, many phages and mobile genetic elements (MGEs) encode CRISPR-Cas inhibitors called anti-CRISPRs (Acrs). Nearly all characterized Acrs directly bind Cas proteins to inactivate CRISPR immunity. Here, using functional metagenomic selection, we describe AcrIIA22,...

Descripción completa

Detalles Bibliográficos
Autores principales:	Forsberg, Kevin J., Schmidtke, Danica T., Werther, Rachel, Uribe, Ruben V., Hausman, Deanna, Sommer, Morten O. A., Stoddard, Barry L., Kaiser, Brett K., Malik, Harmit S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545432/ https://www.ncbi.nlm.nih.gov/pubmed/34644300 http://dx.doi.org/10.1371/journal.pbio.3001428

Ejemplares similares

Inhibition mechanisms of CRISPR-Cas9 by AcrIIA17 and AcrIIA18
por: Wang, Xiaoshen, et al.
Publicado: (2021)

Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae
por: Basgall, Erianna M., et al.
Publicado: (2018)

Corrigendum: Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae
por: Basgall, Erianna M., et al.
Publicado: (2018)

Crystal structure of an anti-CRISPR protein, AcrIIA1
por: Ka, Donghyun, et al.
Publicado: (2018)

AcrIIA5 Suppresses Base Editors and Reduces Their Off-Target Effects
por: Liang, Mingming, et al.
Publicado: (2020)

Structural basis of Staphylococcus aureus Cas9 inhibition by AcrIIA14
por: Liu, Hongnan, et al.
Publicado: (2021)

Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor
por: Kim, Iktae, et al.
Publicado: (2018)

Intrinsic disorder is essential for Cas9 inhibition of anti-CRISPR AcrIIA5
por: An, So Young, et al.
Publicado: (2020)

Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing
por: Garcia, Bianca, et al.
Publicado: (2019)

The initiation, propagation and dynamics of CRISPR-SpyCas9 R-loop complex
por: Zeng, Yan, et al.
Publicado: (2018)

Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
por: Mir, Aamir, et al.
Publicado: (2018)

Atomic-scale insights into allosteric inhibition and evolutional rescue mechanism of Streptococcus thermophilus Cas9 by the anti-CRISPR protein AcrIIA6
por: Li, Xinyi, et al.
Publicado: (2021)

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells
por: Tan, Renke, et al.
Publicado: (2020)

Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
por: Forsberg, Kevin J, et al.
Publicado: (2019)

SpiNNTools: The Execution Engine for the SpiNNaker Platform
por: Rowley, Andrew G. D., et al.
Publicado: (2019)

Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
por: Khairil Anuar, Irsyad N. A., et al.
Publicado: (2019)

Spying on the environment.
Publicado: (1995)

The virus spy
por: Hoag, Hannah
Publicado: (2009)

MIS lateral ACR for spinal deformity correction: technique and complication avoidance
por: Ohiorhenuan, Ifije, et al.
Publicado: (2020)

Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins
por: Hatlem, Daniel, et al.
Publicado: (2019)

SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology
por: Kellmann, Sarah-Jane, et al.
Publicado: (2023)

Expression of acrA and acrB Genes in Esherichia coli Mutants with or without marR or acrR Mutations
por: Pourahmad Jaktaji, Razieh, et al.
Publicado: (2013)

Development of SpyTag/SpyCatcher-Bacmid Expression Vector System (SpyBEVS) for Protein Bioconjugations Inside of Silkworms
por: Xu, Jian, et al.
Publicado: (2019)

SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase
por: Si, Meng, et al.
Publicado: (2016)

Structural basis for inhibition of the type I-F CRISPR–Cas surveillance complex by AcrIF4, AcrIF7 and AcrIF14
por: Gabel, Clinton, et al.
Publicado: (2020)

Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates
por: Cebrián, Jorge, et al.
Publicado: (2021)

The spy that went into the cold
Publicado: (1998)

Five spy novels
Publicado: (1962)

Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics
por: Keeble, Anthony H., et al.
Publicado: (2017)

Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction
por: Gallus, Sabrina, et al.
Publicado: (2020)

One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
por: Zhang, Shanwen, et al.
Publicado: (2021)

Acre : the rise and fall of a Palestinian city, 1730-1831 /
por: Philipp, Thomas, 1941-
Publicado: (2001)

In the absence of writhe, DNA relieves torsional stress with localized, sequence-dependent structural failure to preserve B-form
por: Randall, Graham L., et al.
Publicado: (2009)

Simultaneous Exposure–Response Modeling of ACR20, ACR50, and ACR70 Improvement Scores in Rheumatoid Arthritis Patients Treated With Certolizumab Pegol
por: Lacroix, B D, et al.
Publicado: (2014)

Interchangeability of periplasmic adaptor proteins AcrA and AcrE in forming functional efflux pumps with AcrD in Salmonella enterica serovar Typhimurium
por: Alav, Ilyas, et al.
Publicado: (2021)

Tuning SpyTag–SpyCatcher mutant pairs toward orthogonal reactivity encryption
por: Liu, Yajie, et al.
Publicado: (2017)

Spy vs. spy: selecting the best reporter for (19)F NMR competition experiments
por: de Castro, Guilherme Vieira, et al.
Publicado: (2019)

The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence
por: Aspell, Tiger, et al.
Publicado: (2023)

A spy in the family : an erotic comedy /
por: Waugh, Alec 1898-1981
Publicado: (1977)

The mismatch repair endonuclease MutLα tethers duplex regions of DNA together and relieves DNA torsional tension
por: Witte, Scott J, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_tq2frqgofjsmvuls79kfeblice