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Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR
The pandemic of the novel coronavirus disease 2019 (COVID-19) has caused severe harm to the health of people all around the world. Molecular detection of the pathogen, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), played a crucial role in the control of the disease. Reverse transcrip...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545465/ https://www.ncbi.nlm.nih.gov/pubmed/34697653 http://dx.doi.org/10.1007/s00216-021-03680-2 |
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author | Niu, Chunyan Wang, Xia Zhang, Yongzhuo Lu, Lin Wang, Di Gao, Yunhua Wang, Shangjun Luo, Jingyan Jiang, Ying Wang, Nuo Guo, Yong Zhu, Lingxiang Dong, Lianhua |
author_facet | Niu, Chunyan Wang, Xia Zhang, Yongzhuo Lu, Lin Wang, Di Gao, Yunhua Wang, Shangjun Luo, Jingyan Jiang, Ying Wang, Nuo Guo, Yong Zhu, Lingxiang Dong, Lianhua |
author_sort | Niu, Chunyan |
collection | PubMed |
description | The pandemic of the novel coronavirus disease 2019 (COVID-19) has caused severe harm to the health of people all around the world. Molecular detection of the pathogen, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), played a crucial role in the control of the disease. Reverse transcription digital PCR (RT-dPCR) has been developed and used in the detection of SARS-CoV-2 RNA as an absolute quantification method. Here, an interlaboratory assessment of quantification of SARS-CoV-2 RNA was organized by the National Institute of Metrology, China (NIMC), using in vitro transcribed RNA samples, among ten laboratories on six different dPCR platforms. Copy number concentrations of three genes of SARS-CoV-2 were measured by all participants. Consistent results were obtained with dispersion within 2.2-fold and CV% below 23% among different dPCR platforms and laboratories, and Z′ scores of all the reported results being satisfactory. Possible reasons for the dispersion included PCR assays, partition volume, and reverse transcription conditions. This study demonstrated the comparability and applicability of RT-dPCR method for quantification of SARS-CoV-2 RNA and showed the capability of the participating laboratories at SARS-CoV-2 test by RT-dPCR platform. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-021-03680-2. |
format | Online Article Text |
id | pubmed-8545465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-85454652021-10-26 Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR Niu, Chunyan Wang, Xia Zhang, Yongzhuo Lu, Lin Wang, Di Gao, Yunhua Wang, Shangjun Luo, Jingyan Jiang, Ying Wang, Nuo Guo, Yong Zhu, Lingxiang Dong, Lianhua Anal Bioanal Chem Paper in Forefront The pandemic of the novel coronavirus disease 2019 (COVID-19) has caused severe harm to the health of people all around the world. Molecular detection of the pathogen, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), played a crucial role in the control of the disease. Reverse transcription digital PCR (RT-dPCR) has been developed and used in the detection of SARS-CoV-2 RNA as an absolute quantification method. Here, an interlaboratory assessment of quantification of SARS-CoV-2 RNA was organized by the National Institute of Metrology, China (NIMC), using in vitro transcribed RNA samples, among ten laboratories on six different dPCR platforms. Copy number concentrations of three genes of SARS-CoV-2 were measured by all participants. Consistent results were obtained with dispersion within 2.2-fold and CV% below 23% among different dPCR platforms and laboratories, and Z′ scores of all the reported results being satisfactory. Possible reasons for the dispersion included PCR assays, partition volume, and reverse transcription conditions. This study demonstrated the comparability and applicability of RT-dPCR method for quantification of SARS-CoV-2 RNA and showed the capability of the participating laboratories at SARS-CoV-2 test by RT-dPCR platform. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-021-03680-2. Springer Berlin Heidelberg 2021-10-26 2021 /pmc/articles/PMC8545465/ /pubmed/34697653 http://dx.doi.org/10.1007/s00216-021-03680-2 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Paper in Forefront Niu, Chunyan Wang, Xia Zhang, Yongzhuo Lu, Lin Wang, Di Gao, Yunhua Wang, Shangjun Luo, Jingyan Jiang, Ying Wang, Nuo Guo, Yong Zhu, Lingxiang Dong, Lianhua Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR |
title | Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR |
title_full | Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR |
title_fullStr | Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR |
title_full_unstemmed | Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR |
title_short | Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR |
title_sort | interlaboratory assessment of quantification of sars-cov-2 rna by reverse transcription digital pcr |
topic | Paper in Forefront |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545465/ https://www.ncbi.nlm.nih.gov/pubmed/34697653 http://dx.doi.org/10.1007/s00216-021-03680-2 |
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