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A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy

Cholangiocarcinoma is the second most common malignant tumor in the hepatobiliary system. Compared with data on hepatocellular carcinoma, fewer public data and prognostic-related studies on cholangiocarcinoma are available, and effective prognostic prediction methods for cholangiocarcinoma are lacki...

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Autores principales: Zhang, Zi-jian, Huang, Yun-peng, Li, Xiao-xue, Liu, Zhong-tao, Liu, Kai, Deng, Xiao-feng, Xiong, Li, Zou, Heng, Wen, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545875/
https://www.ncbi.nlm.nih.gov/pubmed/34712611
http://dx.doi.org/10.3389/fonc.2021.747445
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author Zhang, Zi-jian
Huang, Yun-peng
Li, Xiao-xue
Liu, Zhong-tao
Liu, Kai
Deng, Xiao-feng
Xiong, Li
Zou, Heng
Wen, Yu
author_facet Zhang, Zi-jian
Huang, Yun-peng
Li, Xiao-xue
Liu, Zhong-tao
Liu, Kai
Deng, Xiao-feng
Xiong, Li
Zou, Heng
Wen, Yu
author_sort Zhang, Zi-jian
collection PubMed
description Cholangiocarcinoma is the second most common malignant tumor in the hepatobiliary system. Compared with data on hepatocellular carcinoma, fewer public data and prognostic-related studies on cholangiocarcinoma are available, and effective prognostic prediction methods for cholangiocarcinoma are lacking. In recent years, ferroptosis has become an important subject of tumor research. Some studies have indicated that ferroptosis plays an important role in hepatobiliary cancers. However, the prediction of the prognostic effect of ferroptosis in patients with cholangiocarcinoma has not been reported. In addition, many reports have described the ability of photodynamic therapy (PDT), a potential therapy for cholangiocarcinoma, to regulate ferroptosis by generating reactive oxygen species (ROS). By constructing ferroptosis scores, the prognoses of patients with cholangiocarcinoma can be effectively predicted, and potential gene targets can be discovered to further enhance the efficacy of PDT. In this study, gene expression profiles and clinical information (TCGA, E-MTAB-6389, and GSE107943) of patients with cholangiocarcinoma were collected and divided into training sets and validation sets. Then, a model of the ferroptosis gene signature was constructed using least absolute shrinkage and selection operator (LASSO)-penalized Cox regression analysis. Furthermore, through the analysis of RNA-seq data after PDT treatment of cholangiocarcinoma, PDT-sensitive genes were obtained and verified by immunohistochemistry staining and Western blot. The results of this study provide new insight for predicting the prognosis of cholangiocarcinoma and screening target genes that enhance the efficacy of PDT.
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spelling pubmed-85458752021-10-27 A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy Zhang, Zi-jian Huang, Yun-peng Li, Xiao-xue Liu, Zhong-tao Liu, Kai Deng, Xiao-feng Xiong, Li Zou, Heng Wen, Yu Front Oncol Oncology Cholangiocarcinoma is the second most common malignant tumor in the hepatobiliary system. Compared with data on hepatocellular carcinoma, fewer public data and prognostic-related studies on cholangiocarcinoma are available, and effective prognostic prediction methods for cholangiocarcinoma are lacking. In recent years, ferroptosis has become an important subject of tumor research. Some studies have indicated that ferroptosis plays an important role in hepatobiliary cancers. However, the prediction of the prognostic effect of ferroptosis in patients with cholangiocarcinoma has not been reported. In addition, many reports have described the ability of photodynamic therapy (PDT), a potential therapy for cholangiocarcinoma, to regulate ferroptosis by generating reactive oxygen species (ROS). By constructing ferroptosis scores, the prognoses of patients with cholangiocarcinoma can be effectively predicted, and potential gene targets can be discovered to further enhance the efficacy of PDT. In this study, gene expression profiles and clinical information (TCGA, E-MTAB-6389, and GSE107943) of patients with cholangiocarcinoma were collected and divided into training sets and validation sets. Then, a model of the ferroptosis gene signature was constructed using least absolute shrinkage and selection operator (LASSO)-penalized Cox regression analysis. Furthermore, through the analysis of RNA-seq data after PDT treatment of cholangiocarcinoma, PDT-sensitive genes were obtained and verified by immunohistochemistry staining and Western blot. The results of this study provide new insight for predicting the prognosis of cholangiocarcinoma and screening target genes that enhance the efficacy of PDT. Frontiers Media S.A. 2021-10-12 /pmc/articles/PMC8545875/ /pubmed/34712611 http://dx.doi.org/10.3389/fonc.2021.747445 Text en Copyright © 2021 Zhang, Huang, Li, Liu, Liu, Deng, Xiong, Zou and Wen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Zhang, Zi-jian
Huang, Yun-peng
Li, Xiao-xue
Liu, Zhong-tao
Liu, Kai
Deng, Xiao-feng
Xiong, Li
Zou, Heng
Wen, Yu
A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy
title A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy
title_full A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy
title_fullStr A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy
title_full_unstemmed A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy
title_short A Novel Ferroptosis-Related 4-Gene Prognostic Signature for Cholangiocarcinoma and Photodynamic Therapy
title_sort novel ferroptosis-related 4-gene prognostic signature for cholangiocarcinoma and photodynamic therapy
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545875/
https://www.ncbi.nlm.nih.gov/pubmed/34712611
http://dx.doi.org/10.3389/fonc.2021.747445
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