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Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization

Fluorescence in situ hybridization (FISH) is a powerful tool to visualize transcripts in fixed cells and tissues. Despite the recent advances in FISH detection methods, it remains challenging to achieve high-level FISH imaging with a simple workflow. Here, we introduce a method to prepare long singl...

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Detalles Bibliográficos
Autores principales: Cao, Dongjian, Wu, Sa, Xi, Caili, Li, Dong, Zhu, Kaiheng, Zhang, Zhihong, Gong, Hui, Luo, Qingming, Yang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545947/
https://www.ncbi.nlm.nih.gov/pubmed/34697406
http://dx.doi.org/10.1038/s42003-021-02762-2