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Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress

Soil phosphate (Pi) deficiency is a global issue and a major constraint on plant growth. Plants typically acclimatize to low Pi by enhancing their P utilization and/or P acquisition efficiencies; however, different species have variable preferred strategies. RNA sequencing analysis was performed on...

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Autores principales: Hu, Xiaowei, Zhang, Lijing, Niu, Decao, Nan, Shuzhen, Wu, Shujuan, Gao, Hongjuan, Fu, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545990/
https://www.ncbi.nlm.nih.gov/pubmed/34712251
http://dx.doi.org/10.3389/fpls.2021.723595
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author Hu, Xiaowei
Zhang, Lijing
Niu, Decao
Nan, Shuzhen
Wu, Shujuan
Gao, Hongjuan
Fu, Hua
author_facet Hu, Xiaowei
Zhang, Lijing
Niu, Decao
Nan, Shuzhen
Wu, Shujuan
Gao, Hongjuan
Fu, Hua
author_sort Hu, Xiaowei
collection PubMed
description Soil phosphate (Pi) deficiency is a global issue and a major constraint on plant growth. Plants typically acclimatize to low Pi by enhancing their P utilization and/or P acquisition efficiencies; however, different species have variable preferred strategies. RNA sequencing analysis was performed on the shoots and roots of Zygophyllum xanthoxylum, under 1 day and 10 days of Pi stress, to investigate their adaptation strategies to P deprivation. A total of 364,614 unigenes and 9,270 differentially expressed genes (DEGs) were obtained via transcriptome sequencing. An analysis of the DEGs revealed that under the 10D treatment, anthocyanin synthesis genes were upregulated under Pi stress, whereas gibberellin, ethylene, and cytokinins synthesis genes were upregulated, and abscisic acid synthesis genes were downregulated. Genes related to organic acid synthesis, encoding for purple acid phosphatases (APase) and nucleases (RNase) were upregulated under the 1D and 10D treatments, respectively. Furthermore, genes associated with Pi transport were induced by Pi stress. Zygophyllum xanthoxylum has special P adaptation strategies, the variation trends of genes involved in external P mobilization and acquisition, which were different from that of most other species; however, the expression levels of organophosphorus mobilization related genes, such as APases and RNases, were significantly increased. Meanwhile, PHT2s and TPTs, which distributed Pi to effective sites (e.g., chloroplast), played critical roles in the maintenance of photosynthesis. We speculated that these were economic and energy saving strategies, and there are critical adaptive mechanisms that Z. xanthoxylum employs to cope with deficits in Pi.
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spelling pubmed-85459902021-10-27 Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress Hu, Xiaowei Zhang, Lijing Niu, Decao Nan, Shuzhen Wu, Shujuan Gao, Hongjuan Fu, Hua Front Plant Sci Plant Science Soil phosphate (Pi) deficiency is a global issue and a major constraint on plant growth. Plants typically acclimatize to low Pi by enhancing their P utilization and/or P acquisition efficiencies; however, different species have variable preferred strategies. RNA sequencing analysis was performed on the shoots and roots of Zygophyllum xanthoxylum, under 1 day and 10 days of Pi stress, to investigate their adaptation strategies to P deprivation. A total of 364,614 unigenes and 9,270 differentially expressed genes (DEGs) were obtained via transcriptome sequencing. An analysis of the DEGs revealed that under the 10D treatment, anthocyanin synthesis genes were upregulated under Pi stress, whereas gibberellin, ethylene, and cytokinins synthesis genes were upregulated, and abscisic acid synthesis genes were downregulated. Genes related to organic acid synthesis, encoding for purple acid phosphatases (APase) and nucleases (RNase) were upregulated under the 1D and 10D treatments, respectively. Furthermore, genes associated with Pi transport were induced by Pi stress. Zygophyllum xanthoxylum has special P adaptation strategies, the variation trends of genes involved in external P mobilization and acquisition, which were different from that of most other species; however, the expression levels of organophosphorus mobilization related genes, such as APases and RNases, were significantly increased. Meanwhile, PHT2s and TPTs, which distributed Pi to effective sites (e.g., chloroplast), played critical roles in the maintenance of photosynthesis. We speculated that these were economic and energy saving strategies, and there are critical adaptive mechanisms that Z. xanthoxylum employs to cope with deficits in Pi. Frontiers Media S.A. 2021-10-12 /pmc/articles/PMC8545990/ /pubmed/34712251 http://dx.doi.org/10.3389/fpls.2021.723595 Text en Copyright © 2021 Hu, Zhang, Niu, Nan, Wu, Gao and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Hu, Xiaowei
Zhang, Lijing
Niu, Decao
Nan, Shuzhen
Wu, Shujuan
Gao, Hongjuan
Fu, Hua
Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress
title Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress
title_full Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress
title_fullStr Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress
title_full_unstemmed Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress
title_short Transcriptome Analysis of Zygophyllum xanthoxylum Adaptation Strategies to Phosphate Stress
title_sort transcriptome analysis of zygophyllum xanthoxylum adaptation strategies to phosphate stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545990/
https://www.ncbi.nlm.nih.gov/pubmed/34712251
http://dx.doi.org/10.3389/fpls.2021.723595
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