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Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling

Liver fibrosis is a progression stage of chronic liver disease, while current therapies cannot cure or attune cirrhosis effectively. Human amniotic mesenchymal stromal cell (hAMSC) presented immunoregulatory and tissue repairability of multiple illnesses. Regulatory T cells (Treg) had been proved to...

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Autores principales: Deng, Zhenhua, Zhou, Jinren, Mu, Xiaoxin, Gu, Jian, Li, Xiangyu, Shao, Qing, Li, Jinyang, Yang, Chao, Han, Guoyong, Zhao, Jie, Xia, Yongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545991/
https://www.ncbi.nlm.nih.gov/pubmed/34712665
http://dx.doi.org/10.3389/fcell.2021.737825
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author Deng, Zhenhua
Zhou, Jinren
Mu, Xiaoxin
Gu, Jian
Li, Xiangyu
Shao, Qing
Li, Jinyang
Yang, Chao
Han, Guoyong
Zhao, Jie
Xia, Yongxiang
author_facet Deng, Zhenhua
Zhou, Jinren
Mu, Xiaoxin
Gu, Jian
Li, Xiangyu
Shao, Qing
Li, Jinyang
Yang, Chao
Han, Guoyong
Zhao, Jie
Xia, Yongxiang
author_sort Deng, Zhenhua
collection PubMed
description Liver fibrosis is a progression stage of chronic liver disease, while current therapies cannot cure or attune cirrhosis effectively. Human amniotic mesenchymal stromal cell (hAMSC) presented immunoregulatory and tissue repairability of multiple illnesses. Regulatory T cells (Treg) had been proved to be functional in reducing immune cell activity. We showed that co-infusion of hAMSC and Treg prevented mild liver fibrosis comparing with hAMSC or Treg alone group. In vitro study indicated that the addition of Treg or the supernatant of Treg improved the hepatocyte growth factor (HGF) secreting and cell differentiation ability of hAMSC. Reduction of TGF-β significantly decreased the HGF secreting and differentiation of hAMSC. Multiple signal neutralizers were added to the culture to understand further the mechanism, which showed that 1-MT, the suppressor of Indoleamine 2,3-dioxygenase (IDO), was involved in the effect of TGF-β in regulating hAMSC. Depletion of TGF-β or IDO signaling successfully abolished the effect of Treg in improving hAMSC’s function both in vitro and vivo. Finally, our result indicated that Treg improved the function of hAMSC by regulating the TGF-β-IDO signaling and co-infusion of hAMSC and Treg provided a promising approach for treating liver cirrhosis.
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spelling pubmed-85459912021-10-27 Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling Deng, Zhenhua Zhou, Jinren Mu, Xiaoxin Gu, Jian Li, Xiangyu Shao, Qing Li, Jinyang Yang, Chao Han, Guoyong Zhao, Jie Xia, Yongxiang Front Cell Dev Biol Cell and Developmental Biology Liver fibrosis is a progression stage of chronic liver disease, while current therapies cannot cure or attune cirrhosis effectively. Human amniotic mesenchymal stromal cell (hAMSC) presented immunoregulatory and tissue repairability of multiple illnesses. Regulatory T cells (Treg) had been proved to be functional in reducing immune cell activity. We showed that co-infusion of hAMSC and Treg prevented mild liver fibrosis comparing with hAMSC or Treg alone group. In vitro study indicated that the addition of Treg or the supernatant of Treg improved the hepatocyte growth factor (HGF) secreting and cell differentiation ability of hAMSC. Reduction of TGF-β significantly decreased the HGF secreting and differentiation of hAMSC. Multiple signal neutralizers were added to the culture to understand further the mechanism, which showed that 1-MT, the suppressor of Indoleamine 2,3-dioxygenase (IDO), was involved in the effect of TGF-β in regulating hAMSC. Depletion of TGF-β or IDO signaling successfully abolished the effect of Treg in improving hAMSC’s function both in vitro and vivo. Finally, our result indicated that Treg improved the function of hAMSC by regulating the TGF-β-IDO signaling and co-infusion of hAMSC and Treg provided a promising approach for treating liver cirrhosis. Frontiers Media S.A. 2021-10-12 /pmc/articles/PMC8545991/ /pubmed/34712665 http://dx.doi.org/10.3389/fcell.2021.737825 Text en Copyright © 2021 Deng, Zhou, Mu, Gu, Li, Shao, Li, Yang, Han, Zhao and Xia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Deng, Zhenhua
Zhou, Jinren
Mu, Xiaoxin
Gu, Jian
Li, Xiangyu
Shao, Qing
Li, Jinyang
Yang, Chao
Han, Guoyong
Zhao, Jie
Xia, Yongxiang
Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling
title Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling
title_full Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling
title_fullStr Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling
title_full_unstemmed Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling
title_short Regulatory T Cells Improved the Anti-cirrhosis Activity of Human Amniotic Mesenchymal Stem Cell in the Liver by Regulating the TGF-β-Indoleamine 2,3-Dioxygenase Signaling
title_sort regulatory t cells improved the anti-cirrhosis activity of human amniotic mesenchymal stem cell in the liver by regulating the tgf-β-indoleamine 2,3-dioxygenase signaling
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545991/
https://www.ncbi.nlm.nih.gov/pubmed/34712665
http://dx.doi.org/10.3389/fcell.2021.737825
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