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Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes

BACKGROUND: Wild medicinal plants are suffering natural environmental stresses and habitat destruction. The genetic diversity evaluation of wild accessions and their in vitro raised genotypes using molecular markers, as well as the estimation of substances of pharmaceutical value in wild plants and...

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Autores principales: Badr, Abdelfattah, El-Shazly, Hanaa H., Sakr, Mahmoud, Farid, Mai M., Hamouda, Marwa, Elkhateeb, Eman, Ahmad, Hanan Syed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546000/
https://www.ncbi.nlm.nih.gov/pubmed/34694505
http://dx.doi.org/10.1186/s43141-021-00267-3
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author Badr, Abdelfattah
El-Shazly, Hanaa H.
Sakr, Mahmoud
Farid, Mai M.
Hamouda, Marwa
Elkhateeb, Eman
Ahmad, Hanan Syed
author_facet Badr, Abdelfattah
El-Shazly, Hanaa H.
Sakr, Mahmoud
Farid, Mai M.
Hamouda, Marwa
Elkhateeb, Eman
Ahmad, Hanan Syed
author_sort Badr, Abdelfattah
collection PubMed
description BACKGROUND: Wild medicinal plants are suffering natural environmental stresses and habitat destruction. The genetic diversity evaluation of wild accessions and their in vitro raised genotypes using molecular markers, as well as the estimation of substances of pharmaceutical value in wild plants and their regenerated genotypes are convenient approaches to test the genetic fidelity of regenerated plants as a source of substances of pharmaceutical value. In this study, the genetic diversity of 12 accessions of the medicinal plant Achillea fragrantissima, representing five sites in the mountains of South Sinai, Egypt, were estimated by the inter simple sequence repeats (ISSR) fingerprinting and their volatile oil components were identified using gas chromatography-mass spectrometry (GC-MS) analysis. The same accessions were regenerated in vitro and the genetic diversity and volatile oil components of propagated genotypes were determined and compared to their wild parents. RESULTS: Clustering and principal component analyses indicated that the wild accessions and their regenerated genotypes were genetically differentiated, but the regenerated plants are relatively more diverse compared to their wild parents. However, genetic variation between wild accessions is inherited to their in vitro propagated genotypes indicating genotypic differentiation of the examined accessions. The number of volatile oil compounds in the wild A. fragrantissima accessions was 31 compounds while in the in vitro propagated plants only 24 compounds were detected. Four major compounds are common to both wild and regenerated plants; these are artemisia ketone, alpha-thujone, dodecane, and piperitone. CONCLUSIONS: Genome profiling and essential oil components analysis showed variations in A. fragrantissima accessions from different populations. Genetic differences between wild and regenerated genotypes were analyzed and validated with the final conclusion that in vitro conditions elicited higher genetic variation that is associated with reduced amount and diversity in the essential oil components. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-021-00267-3.
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spelling pubmed-85460002021-11-10 Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes Badr, Abdelfattah El-Shazly, Hanaa H. Sakr, Mahmoud Farid, Mai M. Hamouda, Marwa Elkhateeb, Eman Ahmad, Hanan Syed J Genet Eng Biotechnol Research BACKGROUND: Wild medicinal plants are suffering natural environmental stresses and habitat destruction. The genetic diversity evaluation of wild accessions and their in vitro raised genotypes using molecular markers, as well as the estimation of substances of pharmaceutical value in wild plants and their regenerated genotypes are convenient approaches to test the genetic fidelity of regenerated plants as a source of substances of pharmaceutical value. In this study, the genetic diversity of 12 accessions of the medicinal plant Achillea fragrantissima, representing five sites in the mountains of South Sinai, Egypt, were estimated by the inter simple sequence repeats (ISSR) fingerprinting and their volatile oil components were identified using gas chromatography-mass spectrometry (GC-MS) analysis. The same accessions were regenerated in vitro and the genetic diversity and volatile oil components of propagated genotypes were determined and compared to their wild parents. RESULTS: Clustering and principal component analyses indicated that the wild accessions and their regenerated genotypes were genetically differentiated, but the regenerated plants are relatively more diverse compared to their wild parents. However, genetic variation between wild accessions is inherited to their in vitro propagated genotypes indicating genotypic differentiation of the examined accessions. The number of volatile oil compounds in the wild A. fragrantissima accessions was 31 compounds while in the in vitro propagated plants only 24 compounds were detected. Four major compounds are common to both wild and regenerated plants; these are artemisia ketone, alpha-thujone, dodecane, and piperitone. CONCLUSIONS: Genome profiling and essential oil components analysis showed variations in A. fragrantissima accessions from different populations. Genetic differences between wild and regenerated genotypes were analyzed and validated with the final conclusion that in vitro conditions elicited higher genetic variation that is associated with reduced amount and diversity in the essential oil components. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-021-00267-3. Springer Berlin Heidelberg 2021-10-25 /pmc/articles/PMC8546000/ /pubmed/34694505 http://dx.doi.org/10.1186/s43141-021-00267-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Badr, Abdelfattah
El-Shazly, Hanaa H.
Sakr, Mahmoud
Farid, Mai M.
Hamouda, Marwa
Elkhateeb, Eman
Ahmad, Hanan Syed
Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes
title Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes
title_full Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes
title_fullStr Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes
title_full_unstemmed Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes
title_short Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes
title_sort genetic diversity and volatile oil components variation in achillea fragrantissima wild accessions and their regenerated genotypes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546000/
https://www.ncbi.nlm.nih.gov/pubmed/34694505
http://dx.doi.org/10.1186/s43141-021-00267-3
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